• 한국식품위생안전성학회지
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• 제34권5호
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• pp.502-507
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• 2019

본 연구는 국내에 유통되는 두족류 제품에 대한 모니터링을 수행하였다. 문어와 오징어로 표기되어 판매되는 28개 제품을 대상으로 DNA 바코드를 분석하여 원재료의 종을 동정하였다. DNA 바코드 증폭을 위하여 미토콘드리아의 16S ribosomal RNA 및 cytochrome c oxidase subunit I 유전자 부위를 증폭하는 두 종류의 프라이머 세트를 선정하였으며, 이를 이용하여 증폭된 PCR 산물의 염기서열을 분석하였다. 확보한 염기서열은 'BLAST Search'를 이용하여 미국국립보건원 GenBank에 등록되어있는 생물 종의 염기서열과 비교하여 유사도와 매칭 점수를 고려하여 최종 종을 동정하였다. 동정결과, 원재료를 오징어로 표기한 12개 제품은 아메리카대왕오징어(Dosidicus gigas, n=3), 살오징어(Todarodes pacificus, n=9) 종으로 확인되었다. 반면 문어를 원재료로 표기한 16개 제품의 경우, 6개 제품에서 대만주머니낙지(Cistopus taiwanicus, n=1), 하이야주꾸미(Amphioctopus marginatus, n=1), Scaeurgus unicirrhus(n=1), 아메리카대왕오징어(Dosidicus gigas, n=3)로 동정되어 표기된 원재료와 불일치하였으며, 이 중 3개의 제품은 알레르기 유발 원재료인 오징어가 사용되었음을 확인하였다.

• Journal of Applied Biological Chemistry
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• 제42권3호
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• pp.119-124
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• 1999

About two thirds of the naturally occurring antibiotics have been discovered from actinomycetes. Therefore, the probability of discovering further new antibiotics from actinomycetes is declining as many known metabolites are isolated repeatedly. However, various efforts leave been made in order to enhance the probability of discovering novel compounds. In the present study, we have developed new screening strategies based on the antibiotic biosynthetic pathway, and the genetic information, utilizing polymerase chain reaction. We have selected macrolide type polyketides. In order to divide the ansamycin group antibotic of macrolide type polyketides, we have selected 3-amino-5-hydroxybenzoic acid (AHBA) moiety which contains a biosynthetically unique structural element in the group as a target molecules. Oligonucleotide primers were designed to amplify DNA fragments of macrolide type polyketide synthase and AHBA synthase genes from fourteen actinomycetes species. This method was successfully applied to all three of the known macrolide type polyketide produccing actinomycetes tested. In addition, it also identified the presence of potential macrolide type polyketide producing genes from seven actinomycetes that were known to produce none of macrolide type polyketides, and AHBA biosynthetic genes in one actinomycetes. This technique is potentially useful for the screening of new antibiotices and cloning of their biosynthetic genes.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2007년도 추계학술발표대회 논문집
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• pp.216-219
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• 2007

New paradigm of modern society today - environmentally sound and sustainable development - led to the attempt on the introduction of natural elements in various spaces by people's interest in the quality of life. As one of the distinguished space marketing in designing the outer space of resort, this plan attempts to limit artificial boundary within open scenery, thus creating nature within nature - interior theme garden. In addition, this plan is aimed to obtain location property of inner garden through limited boundary, to promote the identity of resort by endowing it with new values in space and to form emotion care garden and culture garden for users. The plan has proceeded under the following concepts. 1) Differentiation from outer space has been made by creating a theme garden with unique theme garden - landscape elements and an attempt have been made to increase the values of interior garden through the introduction of new species of plants and the state-of-the-art facilities. 2) Emotion care garden - The garden has been created to get emotion purified systematically within nature and let people take a rest and enjoy their spare time. 3) Culture garden - The garden has been planned where culture could be created through various experience activities as a space with education and entertainment by providing a variety of interesting elements.

• Journal of Photoscience
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• 제9권2호
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• pp.341-343
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• 2002

Bacteriochlorophyll(BChl)-c containing species of green sulfur photosynthetic bacterium Chlorobium (ChI.) vibrioforme, which has BChl-d mainly, was detected. We obtained colonies on agar plates by spreading the liquid culture of ChI. vibrioforme f. sp. thiosulfatophilum strain NCIB 8327 which contained the high ratio of BChl-c/BChl-d, and transferred each colony into a new liquid medium. These cultures after growing were found to be classified into two categories. One possessed BChl-d as a light-harvesting pigment and the other did BChl-c. No colonies examined here contained both BChls-d and c. Therefore, the presence of both BChls-d and c in our cultures of ChI. vibrioforme was ascribed to the coexistence of two different cells which had BChl-d and c as the chlorosomal pigment, respectively. The change of pigment composition observed in our liquid cultures can be thus explained by the difference of growth rates between two kinds of cells.

• 한국어류학회지
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• 제32권2호
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• pp.110-114
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• 2020

우리나라 거제도 연안 수심 4 m 진흙바닥에서 Clariger chionomaculatus 1개체(체장 40.5 mm)를 채집하였다. 본 종의 특징은 가늘고 긴 체형에 비늘이 없고, 눈 아래에 수염이 있으며, 제2등지느러미 기조수가 I, 12~15, 뒷지느러미 기조수는 I, 12~14, 가슴지느러미 상부에 1개의 유리연조, 등 쪽에 다수의 흰색 반점들이 있는 채색패턴을 갖는 점이다. 본 종은 지금까지 일본에서만 보고된 종으로 우리나라에서는 거제도 연안에서 처음 출현하였다. 본종의 신한국명으로 '흰점왜망둑'을 제안한다.

• 한국환경과학회지
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• 제26권6호
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• pp.711-717
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• 2017

Dielectric Barrier Discharge (DBD) plasma is a new technique for use in environmental pollutant degradation, which is characterized by the production of hydroxyl radicals as the primary degradation species. Due to the short lifetime of the chemically active species generated during the plasma reaction, the dissolution of the plasma gas has a significant effect on the reaction performance. The plasma reaction performance can be enhanced by combining the basic plasma reactor with a homogenizer system in which the bubbles are destroyed and turned into micro-bubbles. For this purpose, the improvement of the dissolution of plasma gas was evaluated by measuring the RNO (N-dimethyl-4-nitrosoaniline, an indicator of the generation of OH radicals). Experiments were conducted to evaluate the effects of the diameter, rotation speed, and height of the homogenizer, pore size, and number of the diffuser and the applied voltage on the plasma reaction. The results showed that the RNO removal efficiency of the plasma reactor combined with a homogenizer is two times higher than that of the conventional one. The optimum rotor size and rotation speed of the homogenizer were 15.1 mm, and 19,700 rpm, respectively. Except for the lowest pore size distribution of $10-16{\mu}m$, the pore size of the diffuser showed little effect on RNO removal.

• Parasites, Hosts and Diseases
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• 제43권4호
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• pp.161-164
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• 2005

A survey was performed in order to determine the infection status of the metacercariae of heterophyid fluke in two goby species, Boleophthalmus pectinirostris and Scartelaos sp., collected from Gangjin-gun, and Shinan­gun, Sooncheon-shi, Jeollanam-do, Republic of Korea. A total of three metacercariae of Heterophyopsis continua was found in only one B. pectinirostris $(10.0\%)$ from Gangjin-gun. Heterophyes nocens metacercariae were detected in 24 B. pectinirostris $(96.0\%)$ and 14 Scartelaos sp. $(63.6\%)$ from Shinan-gun. Heterophyopsis continua metacercariae were found in 11 B. pectinirostris $(44.0\%)$ and 21 Scartelaos sp. $(95.5\%)$ from Shinan-gun. Stictodora fuscata metacercariae were detected in 18 B. pectinirostris $(72.0\%)$ from Shinan-gun. No metacercariae were detected in 20 B. pectinirostris from Sooncheon-shi. From the above results, this study is the first to prove that B. pectinirostris and Scartelaos sp. serve as the second intermediate hosts of some heterophyid flukes in Korea.

• Journal of the Korean Wood Science and Technology
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• 제48권6호
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• pp.888-895
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• 2020

Ulmus genus has excellent various physiological activities, including anti-ulcer, antioxidant, antibacterial, anti-cancer, immunity, and homeostasis maintenance effects, and it is known to have many additional drug effects And one of reasons for these excellentbiological activities is a flavan-3-ol chemical group in Ulmus genus. In this study a new flavan-3-ol compound was identified in Ulmus davidiana var. japonica. A flavan-3-ol,(2R,3S)-7-[(2S,3R,4R)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5-diol, named as catechin 7-O-beta-D apiofuranoside, was isolated from the stems and barks of Ulmus davidiana var. japonica for. suberosa, which is a species belonging to the genus Ulmus, growing throughout the Korea peninsula. The structure was elucidated by various spectroscopic methods including high-resolution TOF mass spectrometry, ¹H-NMR and ¹³C-NMR spectrometry and comparison with chemical structures of defined compounds.

• Mycobiology
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• 제42권3호
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• pp.241-248
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• 2014

NADPH oxidases (Noxes), transmembrane proteins found in most eukaryotic species, generate reactive oxygen species and are thereby involved in essential biological processes. However, the fact that genes encoding ferric reductases and ferric-chelate reductases share high sequence similarities and domains with Nox genes represents a challenge for bioinformatic approaches used to identify Nox-encoding genes. Further, most studies on fungal Nox genes have focused mainly on functionality, rather than sequence properties, and consequently clear differentiation among the various Nox isoforms has not been achieved. We conducted an extensive sequence analysis to identify putative Nox genes among 34 eukaryotes, including 28 fungal genomes and one Oomycota genome. Analyses were performed with respect to phylogeny, transmembrane helices, di-histidine distance and glycosylation. Our analyses indicate that the sequence properties of fungal Nox genes are different from those of human and plant Nox genes, thus providing novel insight that will enable more accurate identification and characterization of fungal Nox genes.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.40-56
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• 1995

During the past two decades, China has seen her great progress in plant biotechnology. Since the Chinese market of herb medicine is huge, while the plant resources are shrinking, particular emphasis has been placed in plant tissue and cell cultures of medicinal plants, this includes fast propagation, protoplast isolation and regeneration, cell suspension cultures and large scale fermentation. To optimize culture conditions for producing secondary compounds in vitro, various media, additives and elicitors have been tested. Successful examples of large scale culture for the secondary metabolite biosynthesis are quite limited : Lithospermum ery throrhizon and Arnebia euchroma for shikonin derivatives, Panax ginseng, P. notoginseng, P. quinquefolium for saponins, and a few other medicinal plants. Recent development of genetic transformation systems of plant cells offered a new approach to in vitro production of secondary compounds. Hairy root induction and cultures, by using Ri-plasmid, have been reported from a number of medicinal plant species, such as Artemisia annua that produces little artemisinin in normal cultured cells, and from Glycyrrhiza uralensis. In the coming five years, Chinese scientists will continue their work on large scale cell cultures of a few of selected plant species, including Taxus spp. and A. annua, for the production of secondary metabolites with medicinal interests, one or two groups of scientists will be engaged in molecular cloning of the key enzymes in plant secondary metabolism.