Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Monitoring of Commercial Cephalopod Products Sold on the South Korea Market using DNA Barcode Information

DNA 바코드를 이용한 국내 유통 두족류 제품의 원재료 모니터링 연구

  • Yu, Yeon-Cheol (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation) ;
  • Hong, Yewon (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Jung Ju (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Hyung Soo (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, Tae Sun (Department of Food and Nutrition, College of Health Science, Sangji University)
  • 유연철 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 홍예원 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 김정주 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 김형수 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 강태선 (상지대학교 보건과학대학 식품영양학과)
  • Received : 2019.07.25
  • Accepted : 2019.09.17
  • Published : 2019.10.30
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Abstract

Cephalopods are one of the most important fishery resources in the world because of their desirable taste and nutritional value. In south Korea, one of the countries in which a large amount of seafood is consumed, cephalopods (e.g., octopus, squid, and cuttlefish) have an annual consumption rate of over 400,000 metric tons. In this study, octopus and squid products (n=28) sold on the market were monitored by analyzing sequences of DNA barcode markers (cytochrome c oxidase subunit I and 16S ribosomal RNA genes). For species identification, the NCBI BLAST database was screened with the sequences and analyzed as a query. In this BLAST search, twelve squid products showed 99-100% sequence identity to Dosidicus gigas (n=3) and Todarodes pacificus (n=9). In the case of the other 16 products that were declared using octopus as raw materials on the labels, six products were identified as Cistopus taiwanicus (n=1), Amphioctopus marginatus (n=1), Scaeurgus unicirrhus (n=1), and Dosidicus gigas (n=3). Monitoring results indicated that a significant percentage (37.5%) of mislabeling was present in octopus products sold on the South Korean market.

본 연구는 국내에 유통되는 두족류 제품에 대한 모니터링을 수행하였다. 문어와 오징어로 표기되어 판매되는 28개 제품을 대상으로 DNA 바코드를 분석하여 원재료의 종을 동정하였다. DNA 바코드 증폭을 위하여 미토콘드리아의 16S ribosomal RNA 및 cytochrome c oxidase subunit I 유전자 부위를 증폭하는 두 종류의 프라이머 세트를 선정하였으며, 이를 이용하여 증폭된 PCR 산물의 염기서열을 분석하였다. 확보한 염기서열은 'BLAST Search'를 이용하여 미국국립보건원 GenBank에 등록되어있는 생물 종의 염기서열과 비교하여 유사도와 매칭 점수를 고려하여 최종 종을 동정하였다. 동정결과, 원재료를 오징어로 표기한 12개 제품은 아메리카대왕오징어(Dosidicus gigas, n=3), 살오징어(Todarodes pacificus, n=9) 종으로 확인되었다. 반면 문어를 원재료로 표기한 16개 제품의 경우, 6개 제품에서 대만주머니낙지(Cistopus taiwanicus, n=1), 하이야주꾸미(Amphioctopus marginatus, n=1), Scaeurgus unicirrhus(n=1), 아메리카대왕오징어(Dosidicus gigas, n=3)로 동정되어 표기된 원재료와 불일치하였으며, 이 중 3개의 제품은 알레르기 유발 원재료인 오징어가 사용되었음을 확인하였다.

Keywords

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