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http://dx.doi.org/10.13103/JFHS.2019.34.5.502

Monitoring of Commercial Cephalopod Products Sold on the South Korea Market using DNA Barcode Information  

Yu, Yeon-Cheol (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation)
Hong, Yewon (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation)
Kim, Jung Ju (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation)
Kim, Hyung Soo (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation)
Kang, Tae Sun (Department of Food and Nutrition, College of Health Science, Sangji University)
Publication Information
Journal of Food Hygiene and Safety / v.34, no.5, 2019 , pp. 502-507 More about this Journal
Abstract
Cephalopods are one of the most important fishery resources in the world because of their desirable taste and nutritional value. In south Korea, one of the countries in which a large amount of seafood is consumed, cephalopods (e.g., octopus, squid, and cuttlefish) have an annual consumption rate of over 400,000 metric tons. In this study, octopus and squid products (n=28) sold on the market were monitored by analyzing sequences of DNA barcode markers (cytochrome c oxidase subunit I and 16S ribosomal RNA genes). For species identification, the NCBI BLAST database was screened with the sequences and analyzed as a query. In this BLAST search, twelve squid products showed 99-100% sequence identity to Dosidicus gigas (n=3) and Todarodes pacificus (n=9). In the case of the other 16 products that were declared using octopus as raw materials on the labels, six products were identified as Cistopus taiwanicus (n=1), Amphioctopus marginatus (n=1), Scaeurgus unicirrhus (n=1), and Dosidicus gigas (n=3). Monitoring results indicated that a significant percentage (37.5%) of mislabeling was present in octopus products sold on the South Korean market.
Keywords
Cephalopod; DNA barcode; Food fruad; Species identification;
Citations & Related Records
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1 Berger, E., Aquaculture of Octopus species: present status, problems and perspectives. The Plymouth Student Scientist., 4(1), 384-399 (2011).
2 Chung, I.Y., Seo, Y.B., Yang, J.Y., Kwon, K.S., Kim, G.D., Development and validation of quick and accurate Cephalopods grouping system in fishery products by real-time quantitative PCR based on mitochondrial DNA. J. Food Hyg. Saf., 33(4), 280-288 (2018).   DOI
3 Criscuolo, A., Brisse, S., AlienTrimmer: a tool to quickly and accurately trim off multiple short contaminant sequences from high-throughput sequencing reads. Genomics, 102(5-6), 500-506 (2013).   DOI
4 Korea Fishery Trade Association: Statistics on imports and exports of seafood in the third quarter. 9-11 (2017).
5 Kim, H.J., Results of DNA testing of marine products are 1/3 origin false. Nocut NEWS. Retrieved from https://www.nocutnews.co.kr/news/5145288. Assessed May 3 (2019).
6 Handy, S.M., Deeds, J.R., Ivanova, N.V., Hebert, P.D., Hanner, R., Ormos, A., Yancy, H. F., Single laboratory validated method for DNA-barcoding for the species identification of fish. J. AOAC Int., 94(1), 201-210 (2011).   DOI
7 Dai, L., Zheng, X., Kong, L., Li, Q. I., DNA barcoding analysis of Coleoidea (Mollusca: Cephalopoda) from Chinese waters. Mol. Ecol. Resour., 12(3), 437-447 (2012).   DOI
8 Kim, B.D., Squid value marched high, soaring imports of Humboldt squid from South America. Financial NEWS. Retrieved from http://www.fnnews.com/news/201906111058332228. Assessed June 11 (2019).
9 Palumbi, S.R., Nucleic acids II: the polymerase chain reaction. In: Hillis, D.M., Moritz, C., Mable, B.K. (Ed), Molecular Systematics. Sinauer & Associates, Massachusetts, pp. 205-247, (1996).
10 Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D.N., DNA barcoding Australia's fish species. Philos. Trans. R. Soc. Lond. B. Biol. Sci., 360(1462), 1847-1857 (2005).   DOI
11 Hebert, P.D., Cywinska, A., Ball, S.L., Dewaard, J.R., Biological identifications through DNA barcodes. Proc. Biol. Sci. 270(1512), 313-321 (2003).   DOI
12 Ministry of Food and Drug Safety (MFDS), 2016. Foods Labelling Standards, No. 2016-45, pp. 28.
13 Miyazawa, H., Fukamachi, H., Inagaki, Y., Reese, G., Daul, C.B., Lehrer, S.B., Sakaguchi, M., Identification of the first major allergen of a squid (Todarodes pacificus). J. Allergy Clin. Immunol., 98(5), 948-953 (1996).   DOI
14 Han, M.H., Stop selling Chinese processed products that deceive squid into octopus. Yonhap NEWS. Retrieved from https://www.yna.co.kr/view/AKR20180413128600017?input=1195m. Assessed April 13 (2018).
15 Braid, H.E., McBride, P.D., Bolstad, K.S., Molecular phylogenetic analysis of the squid family Mastigoteuthidae (Mollusca, Cephalopoda) based on three mitochondrial genes. Hydrobiologia. 725(1), 145-164 (2014).   DOI
16 Braley, M., Goldsworthy, S.D., Page, B., Steer, M., Austin, J. J., Assessing morphological and DNA-based diet analysis techniques in a generalist predator, the arrow squid Nototodarus gouldi. Mol. Ecol. Resour., 10(3), 466-474 (2010).   DOI
17 Kim, H.S., Seo, Y.B., Choi, S.S., Kim, J.H., Shin, J.Y., Yang, J.Y., Kim, G.D., Development and validation of multiplex polymerase chain reaction to determine squid species based on 16s rRNA gene. J. Food Hyg. Saf., 30(1), 43-50 (2015).   DOI
18 Ivanova, N.V., Zemlak, T.S., Hanner, R., Hebert, P.D.N., Universal primer cocktails for fish DNA barcoding. Mol. Ecol. Notes., 7(4), 544-548 (2007).   DOI
19 Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R., DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3(5), 294-299 (1994).