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검색결과 870건 처리시간 0.039초

김치발효에서 Weissella 속의 중요성과 앞으로의 연구 과제 (Importance of Weissella Species during Kimchi Fermentation and Future Works)

  • 이강욱;박지영;천지연;한남수;김정환
    • 한국미생물·생명공학회지
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    • 제38권4호
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    • pp.341-348
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    • 2010
  • Weissella 종들은 김치발효중 가장 흔히 검출되는 유산균 들이지만 이들에 대한 연구는 매우 부족하다. 새로운 속으로 비교적 최근에 정립된 점이 연구가 미흡한 한 이유이고 생화학적 특성들에 기초한 동정법의 부정확성도 다른 이유가 된다. 현재 14종이 등록되어 있으나 새로운 종들이 계속 보고되고 있다. Weissella들의 특성과 김치발효중 역할을 상세히 이해하는 것이 중요하며 특히 맛과 기능성이 우수한 김치 제조를 위해 Weissella 균주들의 장점을 충분히 이용하려할 경우 중요하다.

Preliminary research for molecular markers of two invasive toxic weeds, Cenchrus L. (Poaceae) species, based on NGS technique

  • Hyun, JongYoung;Jung, Joonhyung;Do, Hoang Dang Khoa;Kim, Joo-Hwan
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.71-71
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    • 2019
  • The genus Cenchrus (Poaceae), containing ca. 23 species, is distributed throughout Australia, Africa, Indian sub-continent, and America. In Korea, Cenchrus longispinus (Hack.) Fernald, especially introduced to Daecheong Island in 1999, is one of the most hazardous invasive plant which causes serious environmental threats, biodiversity damages and physically negative impact on humans and animals. Based on the next-generation sequencing (NGS) technology, we characterized the chloroplast (cp) genome sequences of C. longispinus which contains a large single copy (LSC; 80,223 bp), a small single copy (SSC; 12,449 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). Additionally, we analyzed the cp genome sequences of Cenchrus echinatus L. which contains a large single copy (LSC; 80,220 bp), a small single copy (SSC; 12,439 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). These cp genomes consist of 75 unique genes, 4 rRNA coding genes, 33 tRNA coding genes and 21 duplicated in the IR regions, of which the gene content and organization are similar to the other Poaceae cp genomes. We selected 40 potential regions in cp genomes of two Cenchrus species and one Korean Pennisetum species to develop new single nucleotide polymorphism (SNP) markers for identifying C. longispinus based on amplification-refractory mutation system (ARMS) technique. The markers, inferred from SNP in matK and ndhF genes, show effectiveness to recognize C. longispinus from C. echinatus and Korean native species Pennisetum alopecuroides (L.) Spreng.

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Infection status and microhabitat of polyopisthocotylean Monogenea found on the gills of goldlined seabream, Rhabdosargus sarba, from Musairah Island, Oman

  • Al Sawafi, Maryam;Cho, Miyoung;Al Sulaimani, Adil;Yoon, Gilha
    • 한국어병학회지
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    • 제34권1호
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    • pp.55-61
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    • 2021
  • One of the major problems in fish production is that parasite-induced episodes of disease can result in significant impacts to the health of stock, mortalities and, economical losses. Seabreams are economically important fish in both the capture fishery and aquaculture sectors in Oman and represent potential species for aquaculture. The current study set out to investigate infection of polyopisthocotylean monogeneans on the gills of wild goldlined seabream, Rhabdosargus sarba, caught from Musairah region in Oman. The prevalence, mean intensity and abundance of polyopisthocotyleans on 145 fish was found to be 52.41%, 5.65 and 3.04, respectively. Three species of microcotylid parasite were isolated, one being Atrispinum acarne while the remaining two species require further study to identify them. There was no significant difference in the infection rate between host sex, however, parasite burdens were higher on larger sized fish (p<0.05) and were found to favour the first gill arch (p< 0.05). The study found that the sample of Rhabdosargus sarba landed in the waters around Musairah are heavily infected, with a predominantly immature population of microcotylids. Further work is required to better understand the potential risk each species poses to the health of its host before a decision is made on the suitability of new candidate fish species for commercial aquaculture.

DNA 생물정보를 이용한 한국산 자리돔과 어류의 분류 및 분자계통학적 위치 (Species Identification and Molecular Phylogenetic Position of Korean Damselfishes (Pomacentridae: Chrominae) Based on DNA Bioinformation)

  • 고정락;박영철
    • 한국어류학회지
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    • 제19권4호
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    • pp.274-285
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    • 2007
  • 자리돔과(Teleostei: Pomacentridae) 어류는 열대 및 아열대 해역에 널리 분포하고 있으며 특히 산호해역의 해양생물 군락을 이루는 주요구성 어류이다. 현재 우리나라에는 본과에 5종이 분포하는 것으로 알려져 있는데 본 논문에서는 genus Chromis와 Dascyllus 2속을 대상으로 분자계통학적 위치를 규명하는데 그 목적이 있다. 분자계통분석에서 일본산 D. aruanus는 계통도상에서 폴리네시아산 D. aruanus와 함께 유집되었고, 우리나라의 Chromis fumea는 호주의 C. nitida와 함께 유집되었으며, 두 종간의 유전적 거리를 표시하는 P 값은 0.047로 상대적으로 매우 낮았다. 우리나라의 C. notatus는 뉴칼레도니아산 C. flavomaculata와 함께 유집되었는데, 특히 제주의 D. melanurus와 인도-태평양의 D. melanurus 사이에는 유전자 염기서열의 차이가 전혀 관찰되지 않았다. 그러나 이 두 지역의 D. melanurs와 인도네시아의 D. melanurus간에는 한개의 염기서열 차이가 있었다. 일본산 Dascyllus aruanus의 염기서열은 폴리네시아산 Dascyllus aruanus와 2개의 염기서열 차이가 있었고, 한국산 Chromis fumea와 C. notatus 사이에는 다소 많은 수의 염기서열 차이가 있었다. 본 논문에서 도입한 미토콘드리아 cytochrome DNA의 염기서열 정보 및 분석은 이 유전자가 종 수준에서 뿐만 아니라 지역집단의 구분 및 확인을 위한 DNA 바코드로서 이용될 수 있음을 보여준다.

한국 연근해어업의 합리적 관리를 위한 LP모형의 도입방안에 관한 연구 (A Study on the Introduction of Linear Programming Model into the Management of Korean Coastal and Offshore Fisheries)

  • 박장일
    • 수산경영론집
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    • 제25권1호
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    • pp.37-59
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    • 1994
  • Many studies to cope with the present problems of Korean coastal and offshore fisheries has been performed, but these were done partly in necessities and general studies for Korean inshore and offshore fisheries are in early stage. Most of these studies adopted analytical way of approach for each fishery individually and they could not reflect the effect of correlated interaction among fisheries on the several common species/stocks, and thus optimal effort allocation was impossible. To consider general fisheries and optimal effort allocation among competing mixed species, a linear programming (LP) approach is applied in this study and introduced into 16 important inshore and offshore fisheries with 13 constraining species which were chosen by annual yield order. This study is not based on the biological interaction among species (i.e., prey - predator system) but the technological interaction between species and fishing efforts. For the application of LP model in these fisheries, the standardization of fishing efforts through different fishing gears could not be successful and a new way of effort standardization through CPUE for vessel tonnage was originated. Total standardized fishing effort on a particular species i, Ei, is computed as the linear summation of standardized fishing effort generated by each fishery j. That is, (equation omitted) where $f_{j}$ is the total vessel tonnage of fishery j and aij is the coefficients contributing to the standardized fishing effort per ton for species i taken in fishery j. The total fishing effort level on species i due to both directed fishing and by - catch can thus be accounted in the aij's. Optimal effort allocation among the j fisheries may be considered a minimizing problem (minimize $\Sigma$ $f_{j}$), subject to the constraints that standardized fishing effort levels on particular species are maintained at, above, and below certain predefined levels. Fishing effort goals for individual species can be based on various biological and/or economic criteria, i.e., fishing effort level generating maximum sustainable yield and/or maximum economic yield. But in this study the $F_{0.1}$ criteria which was accepted as an approximate level for $F_{mey}$ by Outland and Boerema's (1973) study. The findings of this study are, (1) LP model can be applied to the Korean inshore and offshore fisheries giobally. (2) Through a new way of combining multiple different fisheries' efforts for a particular species together generating standardized fishing effort, Schaefer curve could be applied to the complex system successfully. (3) The results of this study for total reduction scale were mostly the same as those of prior studies, but different much from the individual scales of reduction. This study showed the necessities for exploitation of more concrete parameters to put into consideration of profitability of fisheries and social factors, and this model can be modified according to the actual constraints. Also, considering the age structure of stocks, this model can be developed into better one for better fisheries management.ent.

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여우원숭이속(Lepilemuridae)의 핵형 분석을 통해 나타난 Lepilemur 6종(L. mustelinus, L. edwardsi, L. dorsalis, L. leucopus, L. ruficaudatus, L. septentrionalis)의 종 분화 양상 (Speciation Mode Reconstruction for Lepilemur six species (L. mustelinus, L. edwardsi, L. dorsalis, L. leucopus, L. ruficaudatus, L. septentrionalis) Based on the Lepilemur Karyotype Analysis)

  • 정기윤
    • 한국산학기술학회논문지
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    • 제5권2호
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    • pp.141-149
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    • 2004
  • 이 연구의 목적은 Lepilemuridae(여우원숭이속) 4종의 핵형은 그들의 선조격인 두 종의 교잡된 핵형으로부터 형성되었음을 검증하는데 목적이 있다. 여우원숭이 속의 가상적인 선조종의 반수체 핵형은 18개의 상염색체와 x염색체로 구성된다. L. mustelinus(LMU)의 핵형은 염색체 4개가 연속적으로 융합된 염색체와 하나의 Robertsonian 전이 염색체 쌍을 가진다. LSS의 핵형은 단지 2쌍의 상호전위된 염색체를 가지고 있다. 우리들은 LMU와 LSS의 조상핵형(anc LMU and ant LSS)을 재구성할 수 있었고, 그로부터 다른 4종의 Lepilemur가 생성될 수 있었다. anc LMU와 anc LSS의 교잡종은 배우자 형성시 환형의 배치를 거치면서 전혀 다른 형태로 융합된 유전적으로 완전한 배우자를 형성할 수 있다. L. dorsalis의 핵형이 구성되기 위해서는 교잡종의 5조의 Trivalent염색체가 감수분열 중기에 환의 모양으로 배열되면서 인접한 단부동원체들이 융합되어 새로운 핵형인 L. dorsalis의 핵형이 만들어진다. L. leucopus의 핵형은 위와 같이 환모양을 구성하기 위해 배열된 Trivalent 염색체 조들 중에서 단지 한 조가 먼저와 다른 방향으로 환속에 위치하게 되므로써 이웃한 단부동원체들이 융합되어 L. leucopus의 핵형을 형성한다. L. ruficaudatus의 반수체 핵형이 만들어 지는 데에는 환으로 배열될 때 7개의 상동염색체 쌍이 배열되고 이웃하는 단부동원체들의 융합에 의해 새로운 조합으로 된 L. ruficaudatus의 반수체 핵형이 형성된다. L. edwardsi의 반수체 배우자가 형성될 때는 LRE가 만들어 질 때의 환형에서 단지 하나의 삼동염색체 쌍이 분리되므로써 LED의 반수체 핵형이 생성된다. 이러한 기전에 의해서 만들어진 새로운 완전한 배우체들은 동일한 형태의 배우자와 수정되므로써 새로운 상동염색체를 가진 종 L. dorsalis, L. leucopus, L. ruficaudatus, L. edwardsi가 형성되었다 이 결과들은 유전적으로 완전한 새로운 종이 교잡종의 군집으로부터 활성화된 염색체들의 융합, 접합기에서 환형으로의 배열 기전을 통해 형성될 수 있다는 이론을 뒷받침한다.

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해양 생물다양성 정보시스템 개발 -한국 해양생물 종 목록 수립을 중심으로- (Development of the Korea Marine Biodiversity Information System -Focus on the Establishment of the Korea Maine Species Inventory-)

  • 박수영;김성대;이윤호;배세진;박흥식;김충곤
    • Ocean and Polar Research
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    • 제29권3호
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    • pp.273-282
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    • 2007
  • For an efficient management and utilization of marine biodiversity information, we made an attempt to develop the Korea Marine Biodiversity Information System (KoMBIS), building a species name inventory of Korea marine organisms. The inventory includes 17 organism groups: phytoplankton, zooplankton, algae and halophyte, sponges, cnidarians, rotifers, nematodes, bryozoans, brachiopods, molluscs, echiurans, annelids, arthropods, echinoderms, urochordates and fish. The species names were collected from 37 different references and reviewed for validity by taxonomists, which resulted in 9,798 valid names in addition to 1,845 synonyms. The Korea marine species inventory is the first one of this kind, for previous Korean species name inventories were mostly composed of terrestrial and freshwater organisms. KoMBIS, the information system developed, contains not only the species name but also information on morphological and ecological characteristics such as distribution, DNA barcode, and references. This system is convenient for the inputting of new data and servicing users through the internet, so that management and utilization of the biodiversity information is more efficient. Linking the DNA barcode data with species information provides an objective measure for identification of a species, which accommodates the recommendation of Consortium for the Barcode of Life, and makes the Korea marine biodiversity information compatible with international databases. Considering the frequent exchange of marine organisms internationally via ballast water and such issues as climate change, this information system will be useful in many areas of marine biodiversity.

제주도 남부 해역에서 채집된 Ptereleotris evides (망둑어목: 청황문절과) 한국 첫기록 (First Record of Ptereleotris evides (Gobiiformes: Microdesmidae) from the Southern Jejudo Island, Korea)

  • 임민영;권혁준;김진구
    • 한국수산과학회지
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    • 제56권4호
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    • pp.569-574
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    • 2023
  • A single specimen (30.35 mm standard length) of Ptereleotris evides was collected from the southern coast of Jejudo Island, Korea, in September 2017 during a skin diving outing. Ptereleotris evides is characterized by having a slightly elongated body, six spines on the 1st dorsal fin, one spine and 25 soft rays on the 2nd dorsal fin, 22 pectoral fin rays, one spine and four soft rays on the pelvic fin, one spine and 25 soft rays on the anal fin, and no disc in pelvic fin. This species differs from the congeneric species, P. hanae and P. heteroptera in having no elongated rays on the caudal fin and a black spot at the ventral margin of the caudal peduncle, respectively. Molecular analysis results based on mitochondrial DNA cytochrome c oxidase subunit I (COI) sequences showed that our specimen almost corresponded to P. evides (99.82%) sequences from registered at the NCBI. As this is the first record of P. evides in Korean waters, we propose a new Korean name for it, "Ga-mak-cheong-hwang-mun-jeol".

Oleanane Triterpenoids from Gordonia ceylanica

  • Herath, H.M.T.B.;Athukoralage, P.S.
    • Natural Product Sciences
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    • 제4권4호
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    • pp.253-256
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    • 1998
  • Chemical investigation of hot hexane extract of the stem bark of Gordonia ceylanica afforded a new triterpenoid, $3{\beta}-acetoxy-28-hydroxyolean-12-ene$ (1) and three other oleanane triterpenoids, $3{\beta}-hydroxyolean-12-ene$ (2), $3{\beta}-acetoxyolean-12-ene$ (3), and $3{\beta}-acetoxyolean-12-en-11-one$ (4) which are new to the species. Structure of compound 1 was suggested by $^1H\;NMR,\;^{13}C\;NMR$ and MS spectral data and confirmed by converting to previously reported compound, erythrodiol diacetate (5). Structures of 2, 3 and 4 were established by comparison of the spectral data with the previously reported compounds. Further the acid hydrolysate of 4 was identical with $3{\beta}-hydroxyolean-12-en-11-one$ (6).

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Variation in morphological traits over a wave-exposure gradient in one but not in another species of the brown alga Carpophyllum (Fucales)

  • Hodge, Fiona;Buchanan, Joseph;Zuccarello, Giuseppe C.
    • ALGAE
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    • 제26권3호
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    • pp.243-251
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    • 2011
  • Environmental conditions can influence the morphology of local biota through phenotypic plasticity or local adaptation. Macroalgal morphologies are often associated with wave-exposure conditions. We investigated the relationship between morphology and wave exposure in two common endemic subtidal macroalgae, Carpophyllum angustifolium and C. maschalocarpum, from the East Cape of New Zealand. Morphological comparisons were made between individuals from two sites and four different wave-exposure zones, as defined by fetch and barnacle composition. Of the seven morphological traits measured in C. angustifolium, only total length varied, and individuals were longer in more wave-exposed environments between the two exposure zones where the species were found. In contrast, total length, stipe thickness and vesicle presence all varied significantly between exposure zones in C. maschalocarpum. C. maschalocarpum specimens were shorter with thinner stipes, and fewer individuals had vesicles in the more wave-exposed zones. Morphological traits of both species also varied between sites, suggesting that other influences are important for determining species morphology. Further study is needed to investigate the role of phenotypic plasticity and genetic variability for driving morphological variation in C. angustifolium and C. maschalocarpum.