• 한국수산과학회지
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• 제41권5호
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• pp.343-345
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• 2008

This study determined the optimum feeding frequency for the growth of juvenile cherry salmon (Oncorhynchus masou). Triplicate groups of fish (initial mean weight 19.1 g) were fed extruded pellets at four feeding frequencies for 50 days: once every 2 days, once a day, twice a day, and three times a day. The mean water temperature during the feeding trial was $17.9{\pm}1.59^{\circ}C$. The survival of all groups was 100%. The final fork length of fish fed twice a day was significantly greater than that of the other groups (P<0.05). The weight gain of fish fed two and three times daily was significantly greater than that of fish fed once a day or every 2 days (P<0.05). The daily feed intake increased significantly with feeding frequency (P<0.05), but there was no significant difference in the weight gain of fish fed two or three times a day. The feed efficiency of fish fed once every 2 days was highest (P<0.05), and the feed efficiency of fish fed two or three times a day was significantly higher than that of fish fed once a day (P<0.05). The results of this study indicated that the optimum feeding frequency for the growth of juvenile cherry salmon is twice a day, while the feeding efficiency of fish fed once every 2 days was best.

• 한국식품영양과학회지
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• 제38권2호
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• pp.142-145
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• 2009

산천어 70% 에탄올 추출물(MSE)이 암세포 성장억제와 Balb/c 마우스에서의 종양 성장 억제에 미치는 영향을 알아보았다. 인간 자궁암(HeLa), 간암(HepG2), 유방암(MCF-7), 위암(AGS), 폐암(A549) 세포 그리고 인간신장 정상세포 (293)에 MSE을 처리했을 때 세포생존율이 감소하였고 이들 감소율을 MTT assay로 알아보았다. MSE는 정상세포 293에서보다 인간 암세포주인 MCF-7, A549, HepG2, AGS 그리고 HeLa세포에서 현저하게 더 높은 세포독성이 나타났다. 시료 최고농도인 1 mg/mL를 인간 폐암세포(A549), 유방암세포(MCF-7), 간암세포(HepG2) 및 위암세포(AGS)에 처리한 결과 각각 9.2%, 12.7%, 14.6% 및 16.9%의 세포 생존율을 나타내었다. 더하여 복수암 세포(sarcoma-180)를 이용한 실험동물(Balb/c mice)에서의 고형암 성장 억제 효과를 알아보았으며, 각각 25 mg/kg body weight와 250 mg/kg body weight의 MSE를 투여하였을 때 종양 성장 억제율은 각각 44.7%와 55.7%였다. 따라서 우리는 MSE가 암 예방을 위해 인간에게 유익한 기능성 소재일 것이라는 것을 제안하였다.

• 한국어류학회지
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• 제21권4호
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• pp.311-321
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• 2009

Three categories (freshwater, amphidromous, and marine fishes) of Taiwanese fishes are analyzed on the basis of zoogeographic elements, viz. China element, Indo-China element, Indo-West Pacific element, Indo-Pacific element, North-Pacific element, Japan-Oregon element, and circumtropical element. Freshwater fishes, which include the China and Indo-China elements, are distributed on part of the boundary area between the Palaearctic and Oriental regions of Wallace (1876). Diadromous fishes include the North-Pacific, Indo-China and Indo-West Pacific elements. Taiwanese salmon, a landlocked (initially diadromous) species that became established in Taiwan between 0.5 my B.P. and the early Pleistocene, is recognized as a distinct taxon included within the Oncorhynchus masou complex, which comprises here three species and two subspecies, viz. Oncorhynchus masou masou (Sancheoneo, Songeo, Sakura-masu or Yamame), O. masou ishikawae (Satsuki-masu or Amago), O. sp. (Biwa-masu), and O. formosanus (Taiwanese salmon), based on molecular, morphological and biological studies. Marine fishes are discussed under the following headings, brackish-water fishes (fishes of brackish waters and seas adjacent to continental coastlines, North Pacific and Indo-West Pacific elements; fishes of brackish waters and seas primarily around islands, Indo-West Pacific element), reef fishes (fishes of inshore reefs along continental coastlines from 0 to ca.100 m depth, Indo-West Pacific element; fishes of inshore reefs primarily around islands from 0 to ca.100 m depth, Indo-West Pacific element; fishes of offshore reefs along continental shelf edges from ca.150 to 300 m depth, circumtropical and Indo-Pacific elements; fishes of offshore reefs primarily around islands from ca.150 to 300 m depth, Indo-Pacific element), demersal fishes (fishes on continental shelves shallower than ca.150 m depth, Indo-West Pacific and Japan-Oregon elements; fishes on edges and upper continental slopes from ca.150 m to 500 m depth, Indo-West Pacific, Indo-Pacific, and circumtropical elements; fishes on lower continental slopes to abyssal plains from ca.500 m to 6,000 m depth, circumtropical element and rarely Indo-Pacific element), pelagic fishes (epipelagic fishes from 0 to ca.150 m depth, Indo-West Pacific, Indo-Pacific or circumtropical elements; meso- and bathypelagic fishes from ca.150 to 3,000 m depth, circumtropical element). The distribution of Taiwanese marine fishes are influenced by the Kuroshio Current, low-salinity and low-temperature waters from mainland China, and sea-bottom topography.

• Fisheries and Aquatic Sciences
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• 제2권2호
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• pp.176-181
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• 1999

The variations of gene expression and pituitary contents of GTH subunits during the ovarian development of masu salmon, Oncorhynchus masou, were investigated. The pituitary GTHs contents was measured by radioimmunoassays (RIAs) using purified GTH subunits and their antibodies. Pituitary contents of GTH $I\beta$ gradually increased from April through August, and reached the maximum in October. On the other hand, pituitary contents of GTH $II\beta$ remained low until August, but they rapidly increased in October. Total RNAs were prepared from pooled pituitaries and the GTH subunits mRNA in pituitaries was quantified by Northern blot hybridization using masu salmon cDNA probes for each GTH subunit. GTH $I\beta$ mRNA level increased with the progression of ovarian maturity. However, GTH $II\beta$ mRNA was detected only at a more advanced stage, and were extremly high at ovulation. A high levels for GTH a mRNA was detected only at ovulation stage. The synchronous increase in pituitary contents and mRNA levels suggested that ovarian maturity in masu salmon was regulated by both GTH I and GTH II.

• 한국양식학회지
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• 제17권1호
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• pp.62-68
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• 2004

A cDNA encoding the masu salmon, Oncorhynchus masou, estrogen receptor $\alpha$ (msER$\alpha$) was cloned from the pituitary gland by polymerase chain reaction (PCR). This cDNA contains an open reading frame encoding 513 amino acid residues, and the calculated molecular weight of this protein is about 56,430 Dalton. The amino acid sequences of the DNA binding and ligand binding domains of msER$\alpha$ showed high homology to those of other fish species (84-100%). Reverse transcription PCR analysis showed that the mRNA level of msER$\alpha$ in the pituitary was slightly higher in estradiol-17$\beta$(E2) injected masu salmon than that of control fish. To test the biological activity of msER$\alpha$, the cDNA was ligated to a mammalian expression vector and transfected into a gonadotrope-derived cell line, L$\beta$T2, with a reporter plasmid including estrogen responsive element. Expression of the reporter protein, luciferase, was E2 and msER$\alpha$-dependent. The masu salmon ER$\alpha$ is structurally conserved among teleost species and functions as a transcriptional activator in the pituitary cells.

• 한국양식학회지
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• 제12권3호
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• pp.185-191
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• 1999

A genetic analysis for survival in fry and juvenile stages of masu salmon was described. Data from two year-classes of masu salmon were analyzed to estimate the heritability for survival during the fresh water-rearing period. The overall survival for each year-class during 8 months of freshwater rearing were 17.8 and 11.6%, respectively. Whirling disease virus (WDV) was the main cause of death in all year-classes. Survival data obtained for offspring of 42 sires and 60 dams of masu salmon (two year classes of data) was analyzed. Average survival rates in the observation period ranged 2-87% for 1994; 0-98% for 1995, repectively. In both year-classes, heritabilities for survival derived from the sire components of variance were low(0.13-0.18), except one. Heritabilities derived from the dam components of variance ranged 0.14-0.61, including non-additive genetic and /or common enviromental effects. Correlations between survival in two long-term periods were all positive and medium to high in magnitude(0.345-0.918). Correlations between survival in non-succeeding periods were, in general, low and insignificant. Correlation between long-term survival and growth rate was found in masu salmon. The corresponding correlation in masu salmon was not significantly different from zero. Correlations between sire survival and body weight, length and condition factor of slaughter were not significant, but varied.

• 환경생물
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• 제22권1호
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• pp.111-119
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• 2004

2002년 12월부터 2003년 9월까지 파로호의 어류상 및 어류군집을 조사한 결과는 다음과 같다. 조사된 어류는 총 12과 45종이었다. 한국고유종은 H. mylodon, A. yanatsutae, C. herzi 등 15종 (33.33％)이었다. 우점종은 Z. platypus (72.41％), 아우점종은 H. labeo (9.75％)였고 우세종은 O. uncirostris amurensis (5.98％), Z. temmincki (2.42％), R. brunneus (2.01％), H. olidus (0.88％) 등이었다. 채집된 어종들의 생체량은 Z. platypus 82,863.15g, H. labeo 32,942.63 g, O. uncirostris amurensis 27,482.39 g, C. carpio 6,502.87 g, A. japonica 3,022.15 g,S. scherzeri 2,711.05g의 순으로 나타났다. 파로호에서 확인된 도입종은 A.japonica, H. eigenmanni, H. olidus, O. masou masou, C. urotaenius, R. giurinus, C. carpio (Israeli type), C. cuvieri, M. salmoides, L. macrochirus의 10종이었다. 이 중 C. carpio (Israeli type), C. cuvieri, M. salmoides, L. macrochirus는 국외종이며 A. japonica, H. eigenmanni, H. olidus, O. masou masou, C. urotaenius, R. giurinus는 국내 다른 하천으로부터 도입된 종이다.

• 한국수산과학회지
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• 제36권4호
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• pp.346-351
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• 2003

Single-cell gel electrophoresis (comet assay) was used to detect DNA single strand break in blood cells from several marine fish species. Three fish species were collected from Georgia coastal area. Mummichog, Fundulus heteroclitus showed higher DNA damage than sea bass, Lateolabrax japonicus and trout, Oncorhynchus masou masou under the same experimental conditions. Mummichogs had more alkaline-labile sites on their DNA than other fish species. The comet assay with mummichog blood cells at pH 12.5 showed a dose-response curve with the increasing concentrations of hydrogen peroxide. While the isolated leucocytes showed no increase of DNA damage after in vitro exposure to 2-methyl-1,4-naphthoquinone (MNQ), erythrocytes showed dose-dependent DNA damage. These results indicate that the comet assay can be applied successfully as a bioassay using erythrocyte for environmental monitoring.

• 한국수산과학회지
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• 제34권4호
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• pp.419-421
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• 2001

Heritability estimations of masu salmon smelt at underyearing of age were described. Masu salmon was taken from aquaculture station of Mori in Japan. After fertilization, the offsprings in each family were reared in separate egg trays and fingerling tanks. The percentage of fish in each tank which smoltified at 11-month old was recorded, and environmental and genetic factors influencing the percentage smoltification were also studied. Percentage of smoltification was not significantly affected by the number of fish held in a tank, or fish density (correlation coefficient 0.10). However, there was a high correlation ($0.821\~0.565$) between smelt percentage and growth related traits of fish in a family. Heritability estimated from total data was $0.47\pm0.13$, when effects of tanks were ignored.

• Parasites, Hosts and Diseases
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• 제39권4호
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• pp.319-321
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• 2001

Diphyllobothrium latum infection in human is not common in Korea and only thirty seven cases have been reported since 1921. We report two cases of fish tapeworm infection after ingestion of raw cherry salmon (Oncorgynchus masou) caught in the domestic river. Among four family members who ate together raw salmon flesh six months ago, just two, mother and daughter were infected. It is our expectation that the salmon associated tapeworm infections would be enlisted as one of the major parasitic problems with the growing consumption of salmon in Korea.