• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.2457-2460
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• 2003

There is a great demand to manipulate biological cell autonomously since biologist should spend much time to obtain skillful manipulation techniques. For this purpose, we propose a cell chip to control, carry, fix and locate the cell. In this paper, we focus on the cell rotator to rotate individual biological cell based on a micro fluidics technology. The cell rotator consists of injection hole and rotation well to rotate a biological cell properly. Under the variation of flow rate in injection hole, the angular velocity of a biological cell is evaluated to find the feasibility of the proposed rotation method. As a practical experiment, Zebrafish egg is employed. Based on this research, we find the possibility of non-contact rotation way that can highly reduce the damage of the biological cell during manipulation. To realize an autonomous biological cell manipulation, a cell chip with manipulation well and micro channel in this research will be utilized effectively in near future.

• 한국가시화정보학회지
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• 제17권1호
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• pp.69-77
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• 2019

This paper introduces microfluidic manipulation of microorganism by opto-electrokinetic technique, named rapid electrokinetic patterning (REP). REP is a hybrid method that utilizes the simultaneous application of a uniform electric field and a focused laser to manipulate various kinds and types of colloidal particles. Using the technique in preliminary experiments, we have successfully aggregated, translated, and trapped not only spherical polystyrene, latex, and magnetic particles but also ellipsoidal glass particles. Extending the manipulation target to cells, we attempted to manipulate saccharomyces cerevisiae (S. cerevisiae), the most commonly used microorganism for food fermentation and biomass production. As a result, S. cerevisiae were assembled and dynamically trapped by REP at arbitrary location on an electrode surface. It firmly establishes the usefulness of REP technique for development of a high-performance on-chip bioassay system.

• International Journal of Control, Automation, and Systems
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• 제2권2호
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• pp.135-143
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• 2004

Recently, high throughput screening for microorganisms with desired characteristics from a large heterogeneous population has become possible. Single cell separation has taken on increasing significance in recent years, and several different methods have been proposed so far. In this paper, we introduce several cell manipulation methods aiming at single cell separation and investigation. At first, methods for the separation of microorganisms are classified. Then, we introduce two different approaches, that is, (1) indirect manipulation using laser trapped microtools and (2) thermal gelation.

• 대한기계학회논문집A
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• 제36권5호
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• pp.475-479
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• 2012

본 논문은 고차가지구조 폴리머인 AEO3000 를 이용한 UV 광경화 성형 공정을 제안하고자 한다. 이는 기존의 바이오칩 제작에 사용되는 PDMS 보다 경도가 높아 금속 전극 형성이 용이하고 제작 공정이 빠르다는 장점을 갖는다. AEO3000 을 이용하여 본 연구에서는 4 개의 소자를 전기적 유체적으로 연결할 수 있는 전기유체 통합벤치를 제작하고 미소유체 혼합소자와 세포분리소자를 연결, 본 소재와 공정이 바이오칩에 적용될 수 있음을 검증하였다. 전기 유체적 특성 분석 결과 전기적 접촉 저항은 $0.75{\pm}0.44{\Omega}$으로 충분히 작은 값을 보였으며, 유체 접속의 압력 저하는 8.3kPa로 기존의 튜브 연결 방법 대비 39.3% 개선된 값을 보였다. 통합벤치에 접속된 소자에 활성 및 비활성 효모를 주입하여 순차적인 혼합 및 재분리를 성공적으로 구현함으로써 본 소자에 적용된 AEO3000 및 UV 광경화 공정이 생체시료의 처리에 적용될 수 있음을 실험적으로 검증하였다. 이는 바이오 의료 분야에 적용 가능한 생체 친화적 소재의 고속 생산에 응용될 수 있다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제1권4호
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• pp.239-247
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• 2001

The Fluorescence-Activated Cell Sorter (FACS) is a well-established instrument used for identifying, enumerating, classifying and sorting cells by their physical and optical characteristics. For a miniaturized FACS device, a disposable plastic microchip has been developed which has a hydrodynamic focusing chamber using soft lithography. As the characteristics of the spatially confined sample stream have an effect on sample throughput, detection efficiency, and the accuracy of cell sorting, systematic fluid dynamic studies are required. Flow visualization is conducted with a laser scanning confocal microscopy (LSCM), and three-dimensional flow structure of the focused sample stream is reconstructed from 2D slices acquired at $1\mutextrm{m}$ intervals in depth. It was observed that the flow structure in the focusing chamber is skewed by unsymmetrical velocity profile arising from trapezoidal cross section of the microchannel. For a quantitative analysis of a microscopic flow structure, Confocal Micro-PIV system has been developed to evaluate the accelerated flow field in the focusing chamber. This study proposes a method which defines the depth of the measurement volume using a detection pinhole. The trajectories of red blood cells (RBCs) and their interactions with surrounding flow field in the squeezed sample stream are evaluated to find optimal shape of the focusing chamber and fluid manipulation scheme for stable cell transporting, efficient detection, and sorting