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http://dx.doi.org/10.5407/jksv.2019.17.1.069

Opto-electrokinetic Technique for Microfluidic Manipulation of Microorganism  

Kwon, Jae-Sung (Department of Mechanical Engineering, Incheon National University)
Publication Information
Journal of the Korean Society of Visualization / v.17, no.1, 2019 , pp. 69-77 More about this Journal
Abstract
This paper introduces microfluidic manipulation of microorganism by opto-electrokinetic technique, named rapid electrokinetic patterning (REP). REP is a hybrid method that utilizes the simultaneous application of a uniform electric field and a focused laser to manipulate various kinds and types of colloidal particles. Using the technique in preliminary experiments, we have successfully aggregated, translated, and trapped not only spherical polystyrene, latex, and magnetic particles but also ellipsoidal glass particles. Extending the manipulation target to cells, we attempted to manipulate saccharomyces cerevisiae (S. cerevisiae), the most commonly used microorganism for food fermentation and biomass production. As a result, S. cerevisiae were assembled and dynamically trapped by REP at arbitrary location on an electrode surface. It firmly establishes the usefulness of REP technique for development of a high-performance on-chip bioassay system.
Keywords
Rapid electrokinetic patterning; On-chip cell manipulation; Particle polarization; Electrohydrodynamic flow; Electrothermal flow;
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1 Belder, D., 2005." Microfluidics with Droplets." Angew. Chem. Int. Ed.Vol. 44,pp. 3521-3522.   DOI
2 Bhagat, A.A.S., Bow, H., Hou, H.W., Tan, S.J., Han, J. and Lim, C.T., 2010." Microfluidics for cell separation." Medical and Biological Engineering and Computing.Vol. 48,pp. 999-1014.   DOI
3 Gravesen, P., Branebjerg, J. and Jensen, O.S., 1993." Microfluidics-a review." Journal of Micromechanics and Microengineering.Vol. 3,pp. 168-182.   DOI
4 Yi, C., Li, C.-W., Ji, S. and Yang, M., 2006." Microfluidics technology for manipulation and analysis of biological cells." Anal. Chim. Acta.Vol. 560,pp. 1-23.   DOI
5 Gossett, D.R., Tse, H.T.K., Lee, S.A., Ying, Y., Lindgren, A.G., Yang, O.O., Rao, J., Clark, A.T. and Carlo, D.D., 2012." Hydrodynamic stretching of single cells for large population mechanical phenotyping." Proceedings of the National Academy of Sciences.Vol. 109,pp. 7630-7635.   DOI
6 Justin, G., Nasir, M. and Ligler, F.S., 2011." Hydrodynamic and electrical considerations in the design of a four-electrode impedance-based microfluidic device." Analytical and Bioanalytical Chemistry.Vol. 400,pp. 1347-1358.   DOI
7 Tanyeri, M., Ranka, M. and Sittipolkul, N., 2011." A microfluidic-based hydrodynamic trap: design and implementation." Lab Chip.Vol. 11,pp. 1786-1794.   DOI
8 Yamada, M. and Seki, M., 2005." Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics." Lab on a Chip.Vol. 5,pp. 1233-1239.   DOI
9 Furlani, E.P., 2007." Magnetophoretic separation of blood cells at the microscale." J. Phys. D: Appl. Phys.Vol. 40,pp. 1313-1319.   DOI
10 Pshenichnikov, A.F. and Ivanov, A.S., 2012." Magnetophoresis of particles and aggregates in concentrated magnetic fluids." Physical Review E.Vol. 86,pp. 05140101-05140111.
11 Watarai, H., Suwa, M. and Iiguni, Y., 2004." Magnetophoresis and electromagnetophoresis of microparticles in liquids." Analytical and Bioanalytical Chemistry.Vol. 378,pp. 1693-1699.   DOI
12 Deyl, Z., 1982. Electrophoresis. A Survey of Techniques and Applications, Elsevier Science Ltd., New York.
13 Gas, B., 2009." Theory of electrophoresis: Fate of one equation." Electrophoresis.Vol. 30,pp. S7-S15.   DOI
14 Kohlheyer, D., Eijkel, J.C.T., Berg, A.v.d. and Schasfoort, R.B.M., 2008." Miniaturizing free-flow electrophoresis - a critical review." Electrophoresis. Vol. 29,pp. 977-993.   DOI
15 Doh, I. and Cho, Y.-H., 2005." A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process." Sensors and Actuators A.Vol. 121,pp. 59-65.   DOI
16 Gascoyne, P.R.C. and Vykoukal, J., 2002." Particle separation by dielectrophoresis." Electrophoresis.Vol. 23,pp. 1973-1983.   DOI
17 Hu, X., Bessette, P.H., Qian, J., Meinhart, C.D., Daugherty, P.S. and Soh, H.T., 2005." Marker-specific sorting of rare cells using dielectrophoresis." Proceedings of the National Academy of Sciences of the United States of America.Vol. 102,pp. 15757-15761.   DOI
18 Pethig, R., 2007. Cell Physiometry Tools based on Dielectrophoresis, BioMEMS and Biomedical Nanotechnology. pp. 103-126.
19 Zhang, C., Khoshmanesh, K., Mitchell, A. and Kalantar-zadeh, K., 2010." Dielectrophoresis for manipulation of micro/nano particles in microfluidic systems." Analytical and Bioanalytical Chemistry. Vol. 396,pp. 401-420.   DOI
20 Arai, F., Ng, C., Maruyama, H., Ichikawa, A., El-Shimy, H. and Fukuda, T., 2005." On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel." Lab Chip.Vol. 5,pp. 1399-1403.   DOI
21 Block, S.M., Blair, D.F. and Berg, H.C., 1989." Compliance of bacterial flagella measured with optical tweezers." Nature.Vol. 338,pp. 514-518.   DOI
22 Moffitt, J.R., Chemla, Y.R., Smith, S.B. and Bustamante, C., 2008." Recent Advances in Optical Tweezers." Annu. Rev. Biochem.Vol. 77,pp. 205-228.   DOI
23 Williams, S.J., Kumar, A. and Wereley, S.T., 2008." Electrokinetic patterning of colloidal particles with optical landscapes." Lab Chip.Vol. 8,pp. 1879-1882.   DOI
24 Chiou, P.Y., Ohta, A.T. and Wu, M.C., 2005." Massively parallel manipulation of single cells and microparticles using optical images." Nature.Vol. 436,pp. 370-372.   DOI
25 Kwon, J.-S. and Wereley, S.T., 2013." Towards New Methodologies for Manipulation of Colloidal Particles in a Miniaturized Fluidic Device: Optoelectrokinetic Manipulation Technique." J. Fluids Eng.Vol. 135,pp. 0213061-0213010.
26 Kumar, A., Kwon, J.-S., Williams, S.J., Green, N.G., Yip, N.K. and Wereley, S.T., 2010." Optically modulated electrokinetic manipulation and concentration of colloidal particles near an electrode surface." Langmuir.Vol. 26,pp. 5262-5272.   DOI
27 Kumar, A., Williams, S.J., Chuang, H.-S., Green, N.G. and Wereley, S.T., 2011." Hybrid opto-electric manipulation in microfluidicsopportunities and challenges." Lab Chip.Vol. 11,pp. 2135-2148.   DOI
28 Mishra, A., Khor, J.-W., Clayton, K.N., Williams, S.J., Pan, X., Kinzer-Ursem, T. and Wereley, S., 2016." Optoelectric patterning: Effect of electrode material and thickness on laser-induced AC electrothermal flow." Electrophoresis.Vol. 37,pp. 658-665.   DOI
29 Kwon, J.-S., Thakur, R. and Wereley, S.T., 2012. Rapid Electrokinetic Patterning, in: Bhushan, B. (Ed.), Encyclopedia of nanotechnology. Springer, Dordrecht ; New York.
30 Williams, S.J., Kumar, A., Green, N.G. and Wereley, S.T., 2009." A simple, optically induced electrokinetic method to concentrate and pattern nanoparticles." Nanoscale.Vol. 1,pp. 133-137.   DOI
31 Kwon, J.-S., Ravindranath, S.P., Kumar, A., Irudayaraj, J. and Wereley, S.T., 2012." Opto-electrokinetic manipulation for high-performance on-chip bioassays." Lab Chip.Vol. 12,pp. 4955-4959.   DOI
32 Mishra, A., Maltais, T.R., Walter, T.M., Wei, A., Williams, S.J. and Wereley, S.T., 2016." Trapping and viability of swimming bacteria in an optoelectric trap." Lab Chip.Vol. 16,pp. 1039-1046.   DOI
33 Gil, G.-C., Chang, I.-S., Kim, B.H., Kim, M., Jang, J.-K., SooPark, H. and Kim, H.J., 2003." Operational parameters affecting the performannce of a mediator-less microbial fuel cell." Biosens. Bioelectron.Vol. 18,pp. 327-334.   DOI
34 Kim, M.-J., Nam, S.-W., Tamano, K., Machida, M., Kim, S.-K. and Kim, Y.-H., 2011." Optimization for Production of Exo-$\beta$-1,3-glucanase (Laminarinase) from Aspergillus oryzae in Saccharomyces cerevisiae." Korean Society for Biotechnology and Bioengineering. Vol. 26,pp. 427-432.
35 Ristenpart, W.D., Aksay, I.A. and Saville, D.A., 2004." Assembly of colloidal aggregates by electrohydrodynamic flow: Kinetic experiments and scaling analysis." Physical Review E.Vol. 69,pp. 214051-214058.
36 Morgan, H. and Green, N.G., 2002. AC electrokinetics: colloids and nanoparticles, Research Studies Press LTD., Baldock.
37 Rijken, D.C. and Collen, D., 1981." Purification and characterization of the plasminogen activator secreted by human melanoma cells in culture." The Journal of Biological Chemistry.Vol. 256,pp. 7035-7041.   DOI
38 Frizzell, R.A., Rechkemmer, G. and Shoemaker, R.L., 1986." Altered regulation of airway epithelial cell chloride channels in cystic fibrosis." Science.Vol. 233,pp. 558-560.   DOI
39 Morris, G.J., Winters, L., Coulson, G.E. and Clarke, K.J., 1983." Effect of Osmotic Stress on the Ultrastructure and Viability of the Yeast Saccharomyces cerevisiae." J. Gen. Microbiol. Vol. 129,pp. 2023-2034.