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In vivo Study of the Renal Protective Effects of Capsosiphon fulvescens against Streptozotocin-induced Oxidative Stress (스트렙토조토신 유발 당뇨 쥐의 산화스트레스에 대한 매생이 추출물의 신장 보호 효과)

  • Nam, Mi-Hyun;Koo, Yun-Chang;Hong, Chung-Oui;Yang, Sung-Yong;Kim, Se-Wook;Jung, Hye-Lim;Lee, Hwa;Kim, Ji-Yeon;Han, Ah-Ram;Son, Won-Rak;Pyo, Min-Cheol;Lee, Kwang-Won
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.641-647
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    • 2014
  • In this study, we evaluated the effect of Capsosiphon fulvescens extract (CFE) and its active compound, pheophorbide A (PhA), on diabetic kidney failure. Diabetes mellitus (DM) was induced by a single intraperitoneal injection of streptozotocin (STZ; 40 mg/kg body weight (BW)). After a week, the rats were orally administered CFE (4 and 20 mg/kg BW) or PhA (0.2 mg/kg BW) once a day for 9 weeks. After scarification, renal tissue samples were collected for biochemical and histochemical analyses. Our study showed that the treatment with CFE and PhA significantly decreased lipid peroxidation level and the activities of glutathione peroxidase and glutathione-S-transferase (p<0.05), but it increased glutathione level and the activities of glutathione reductase, superoxide dismutase, and catalase in the renal tissues (p<0.05). The CFE- and PhA-treated rats with DM showed improved histochemical appearance and decreased abnormal glycogen accumulation. Therefore, we suggest that PhA-containing CFE could exert renal protective effects against STZ-induced oxidative stress.

Development of Detection Method for Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus using 16S rRNA Gene (16S rRNA를 이용한 다금바리, 자바리, 능성어 판별법 개발)

  • Park, Yong-Chjun;Jung, Yong-Hyun;Kim, Mi-Ra;Shin, Joon-Ho;Kim, Kyu-Heon;Lee, Jae-Hwang;Cho, Tae-Yong;Lee, Hwa-Jung;Lee, Sang-Jae;Han, Sang-Bae
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.1-7
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    • 2013
  • Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus are involved in the Perciformes Order and Serranidae Family. When E. bruneus and E. septemfasciatus are fully grown, the striped pattern on the body gradually disappears. Therefore, morphological classification of adult fishes is quite difficult to identify the differences to N. spinosus. In this study, we investigate the method to differentiate those using PCR. To design the primers, 16S rRNA region of N. spinosus, E. bruneus, and E. septemfasciatus registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for the analysis, Bio Edit ver. 7.0.9.0 was used. As a result, it was design NS-003-F/NS-005-R (136 bp), EB-001-F/EB-002-R (181 bp), and ES-001-F/ES-001-R (123 bp) primers for the differentiation of each 3 different fishes. Therefore, the species-specific primer sets would be a useful tool for scientific and speedy differentiation against the illegal distribution for consumer protection.

Study on Differences in Dietary Habits, and Nutrients Status of Lacto-Ovo Vegetarian and Non-Vegetarian University Students (남자 대학생을 대상으로 한 Lacto-Ovo-Vegetarian과 Non-Vegetarian의 식습관 및 영양상태에 관한 연구)

  • Je, Haejong;Shin, Kyung-Ok
    • The Korean Journal of Food And Nutrition
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    • v.28 no.2
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    • pp.269-280
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    • 2015
  • The purpose of this study was to investigate the dietary habits, health status, and nutrients status of male college students in Seoul according to vegetarian habits. The heights of lacto-ovo vegetarian and non-vegetarian students were not significantly different. However, body weight was significantly higher in the non-vegetarians. The lacto-ovo-vegetarians were vegetarians for religious reasons, and indicated 86.0% satisfaction with their meals. However, the lacto-ovo vegetarian and non-vegetarians showed concerns about low nutrient intakes. Lacto-ovo vegetarians displayed a higher proportion of meal regularity and daily breakfast as compared to non-vegetarians. In addition, their favorite food when eating out was Korean food. Lacto-ovo vegetarians showed higher intakes of milk or other dairy products (yogurt, etc.), vegetables, and fruit, as well as more regular eating habits. In contrast, the non-vegetarians showed higher intakes of high-protein foods (eggs, beans, tofu, etc.) and fatty foods. Lacto-ovo vegetarians showed lower intakes of total energy, as well as vitamins $B_2$, $B_6$, and niacin. Non-vegetarians showed in sufficient dietary fiber intake. In conclusion, lacto-ovo-vegetarians need a meal plan to consider the intake of other nutrients, while non-vegetarians should try to prevent the occurrence of health problems due to excessive intake of nutrients.

Effects of Dietary Sources Containing ω-3 Fatty Acids on the Fatty Acid Composition of Meats in Korean Native Chickens (오메가 3계열 지방산을 함유하는 사료의 급여가 육용 토종닭 계육 내 지방산 조성에 미치는 영향 탐색)

  • Oh, Sung-Taek;Jhun, Heung-Kyu;Park, Jung-Min;Kim, Jin-Man;Kang, Chang-Won;An, Byoung-Ki
    • Food Science of Animal Resources
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    • v.32 no.4
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    • pp.476-482
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    • 2012
  • Estimations were made of oxidative susceptibility and fatty acid composition of edible meats of native chickens fed various dietary sources containing ${\omega}-3$ polyunsaturated fatty acids (PUFA). A total of 240 Korean native chickens were divided into 4 groups, placed into 3 replicates per group, and were fed a commercial diet (Control) or one of the three experimental diets containing 10% perilla meal (PM group), 10% perilla meal-5% full fat flaxseed (PM+FS group), or 10% perilla meal-5% full fat flaxseed-1% fish oil (PM+FS+FO group) for 20 days. Final body weight, weight gain, feed intake, and feed conversion rate among the groups were not significantly different. Dietary treatments did not affect the relative weights of liver, abdominal fat, and breast muscle. The leg weight was increased from the feeding of ${\omega}-3$ PUFA sources. The TBA reactive substance in the edible meat was not different with the dietary treatments. The total ${\omega}-3$ PUFA in chickens that were fed diets containing ${\omega}-3$ PUFA sources increased compared to that of the control. The level of longer chain ${\omega}-3$ PUFAs, such as C20:5 ${\omega}3$ and C22:6 ${\omega}3$ in the PM+FS+FO group, was much higher than that of the others. The addition of local ingredients, such as perilla meal with conventional sources, could be used to obtain value-enhanced meat by enhancing ${\omega}-3$ PUFA.

Effects of circulation exercise on health-related physical fitness, blood pressure and blood vessel elasticity of obese women in the 50s (순환운동이 50대 비만중년여성의 건강체력, 혈압 및 혈관탄성도에 미치는 영향)

  • Kim, Yeon-Joo;Kim, Ji-Hyeon;Ha, Soo-Min;Kim, Jung-Sook;Kim, Jong-Won;Yoon, Byeong-Kon;Kim, Do-Yeon
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.4
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    • pp.756-768
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    • 2017
  • The subjects for the study were twenty four obese women(50's) composed of the exercise group(n=12) and to a "no exercise" control group(n=12). The variables of health-related fitness, blood pressure, and blood vessel elasticity where measured in all the subjects before the start of the training program and after at the end of the 8 weeks circuit training. The test data were analyzed by t-test, paired t-test, and alpha level of p<.05 was set for all tests of significance. Health-related fitness was exercise group, the BMI had significantly decreased, and the 20m shuttle run, sit-ups and sit-and-reach had significantly increased. Blood pressure was exercise group, the systolic blood pressure, and diastolic blood pressure had significantly decreased. Also, after the circuit training program, the exercise group had significantly higher diastolic blood pressure than did the control group. blood vessel elasticity was exercise group, the blood vessel elasticity had significantly decreased. And exercise group had significantly higher diastolic blood vessel elasticity than did the control group. In conclusion, BMI, systolic blood pressure, and diastolic blood pressure had significantly decreased, but 20 m shuttle run, sit-ups and sit-and-reach had significantly increased in the combined exercise group after the 8 weeks circuit training. Therefore, regular and continuous circuit training were effective in improving the body composition, blood pressure, blood vessel elasticity and health-related fitness.

A Preliminary Study on Nutrition Education for Preschool Children in Day-Care Center - Dietary Habit and Nutrition Knowledge - (어린이집 아동의 영양교육을 위한 사전조사 연구 - 식습관과 영양지식을 중심으로 -)

  • Kim, Hye-Kyung;Kim, Jin-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.7
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    • pp.866-873
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    • 2006
  • The purpose of this study was to evaluate the current dietary habits, health related behaviors, nutrition knowledge and body indices, and to investigate the changes in nutrition knowledge for designing and monitoring nutrition education program among preschool children. The subjects were 1,200 preschool children, aged $4{\sim}6$ years. A measurement of the height and weight was conducted. The general home environment, the dietary habit of children and nutrition knowledge were collected using a questionnaire that included information about family income, parent's education and occupations. Using the PIBW, 14.6% of the preschool children were under weight, 54.4% were normal, and 31.0% were overweight or obese. We regarded to food habit score, the highest score was in the regularity of breakfast, while the lowest score was in consumption of yellow-green vegetables such as spinach and carrots. Result in food behavior showed that 69.1% of preschool children had a unbalanced diet, and their favorite snack was fast food like pizza and hamburger then followed soft drink and fried foods (chicken of potato). After nutrition education, there was somewhat improvement in the nutrition knowledge score of preschool children from $7.7{\pm}1.7$ point to $8.9{\pm}1.5$ point. These results suggest that nutrition education help preschool children change their nutrition knowledge. Therefore, it would be needed that new appropriate nutrition education to improve dietary habits and health status.

Optimal Lactic Acid Fermentation Conditions and Quality Properties for Rubus coreanus Miquel (Bokbunja) and Chlorella Mixtures (복분자와 클로렐라 혼합물의 젖산발효 최적조건 및 품질 특성)

  • Kim, Jae-Young;Lee, Sang-Uk;Kim, Na-Hyung;Moon, Kwang-Hyun;Baek, Seung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.3
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    • pp.386-395
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    • 2016
  • To develop a functional fermentation food from Rubus coreanus Miquel (Bokbunja) and chlorella mixtures, optimal lactic acid fermentation conditions were established, and quality properties based on physicochemical evaluation such as chemical compositions, free sugars, organic acids, and antibacterial activities were investigated. Regarding optimal fermentation strain selection, formation of lactic acid was best in Lactobacillus plantarum among the experimental strains (10 kinds), and the optimal fermentation temperature was $37^{\circ}C$. In addition, overall acceptability in the sensory evaluation was highest in the 5% chlorella mixture sample. Therefore, quality properties of the prepared sample under the established optimal fermentation conditions were investigated. Moisture, total sugar (dry basis), crude fiber (dry basis), and pH of fermented Rubus coreanus Miquel juice (RCM) with 5% chlorella mixture (RCM-C5) were reduced by 4.90%, 14.15%, and 0.32%, respectively, as compared with non-fermented RCM. Meanwhile, crude protein, crude fat, and crude ash (dry basis) of RCM-C5 were elevated by 13.75%, 0.18%, and 0.73%, respectively, as compared with RCM. The yellowness (b value) of color values was greater in RCM-C5 compared to RCM. The free sugar and organic acid contents of RCM-C5 were elevated by 0.97% and 616.30 mg%, respectively, as compared with RCM. In addition, the gram positive bacterium Staphylococcus aureus was elevated by 5.83% while gram negative bacteria Escherichia coli and Salmonella Typhimurium were elevated by 2.94% and 4.67%, respectively, as compared with RCM. In conclusion, the quality properties of RCM and chlorella lactic acid fermentation mixtures were improved compared with the general RCM product. Consequently, it is possible to apply fermented RCM as a functional fermentation food.

Studies on the Ecology of Field Mice in Mt. Chuisuh (취서산(鷲棲山) 들쥐류(類)의 생태(生態)에 관한 연구(硏究))

  • Nam, Jung Chil
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.707-716
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    • 2001
  • This study was conducted four ecological survey of field mice of Mt. Chuisuh in Yangsan City, Kyungnam from June, 2000 to March, 2001 for the understanding of field mice damage to forest. The results are obtained as follows. 1. The species of field mice collected were Apodemus agrarius, Apodemus peninsulae and Eothenomys regulus, and Crocidura suaveolens of insectvore. 2. The total collecting rate were 8.1% collected 61 individuals of field mice from 758 trap. 3. The rate among the 61 field mice collected were 45.9% in Apodemus agrarius, 29.5% in Eothenomys regulus, 19.7% in Apodemus peninsulae, and 4.9% in Crocidura suaveolens. 4. The constitution of weight of field mice was 16-51.9g in Apodemus agrarius, 12-25.9g in Apodemus peninsulae, 16-45.9g in Eothenomys regulus, and 12-15.9g in Crocidura suaveolens. 5. The sex ratio of field mice was 48.0% : 52.0% in Apodemus agrarius, 50.0% : 50.0% in Apodemus peninsulae, 55.6% : 44.4% in Eothenomys regulus, and 66.7% : 33.3% in Crocidura suaveolens. 6. The average body lengh was 104.33mm in Eothenomys regulus, 96.04mm in Apodemus agrarius, 94.50mm in Apodemus peninsulae, and 76.33mm in Crocidura suaveolens. 7. The average tail lengh was 92.75mm in Apodemus peninsulae, 80.65mm in Apodemus agrarius, 41.0mm in Crocidura suaveolens, and 40.33mm in Eothenomys regulus. 8. The breeding period of field mice in Mt. Chuisuh presumes from about the early of March to end of October. Specially, The Eothenomys regulus was ascertained the breeding period in December, discovered first time in korea through this experimental study. 9. The average litter size of field mice was 4.5 in Apodemus agrarius, 4.7 in Apodemus peninsulae, and 3.4 in Eothenomys regulus.

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Effect of the Flavonoid Luteolin for Dextran Sodium Sulfate-induced Colitis in NF-${\kappa}B^{EGFP}$ Transgenic Mice (Dextran Sodium Sulfate 유발 장염 모델에서 루테올린의 치료효과)

  • Jang, Byung-Ik
    • Journal of Yeungnam Medical Science
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    • v.23 no.1
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    • pp.26-35
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    • 2006
  • Background: Luteolin, a flavone found in various Chinese herbal medicines is known to possess anti-inflammatory properties through its ability to inhibit various proinflammatory signaling pathways including NF-${\kappa}B$ and p38 MAPK. In this study, we investigated the potential therapeutic effect of luteolin on dextran sodium sulfate (DSS)-induced colitis. Materials and Methods: We used a transgenic mouse model expressing the enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-${\kappa}B$ $cis$-elements. C57BL/6 NF-${\kappa}B^{EGFP}$ mice received 2.5% DSS in their drinking water for six days in combination with daily luteolin administration (1mg/kg body weight, 0.1ml vol, intragastric) or vehicle. NF-${\kappa}B$ activity was assessed macroscopically with a Charge-Coupled Device (CCD) camera and microscopically by confocal analysis. Results: A significant increase in the Disease Activity Index (DAI), histological score (p<0.05), IL-12 p40 secretion in colonic stripe culture (p<0.05) and EGFP expression was observed in luteolin and/or DSS-treated mice compared to water-treated mice. Interestingly, a trend toward a worse colitis (DAI, IL-12p40) was observed in luteolin-treated mice compared to non-treated DSS-exposed mice. In addition, EGFP expression (NF-${\kappa}B$ activity) strongly increased in the luteolin-treated mice compared to control mice. Confocal microscopy showed that EGFP positive cells were primarily lamina propria immune cells. Conclusions: These results suggest that luteolin is not a therapeutic alternative for intestinal inflammatory disorders derived for primary defects in barrier function. Thus, therapeutic intervention targeting these signaling pathways should be viewed with caution.

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Nammyeong Jo-Sik's Practical Social Reformism (남명 조식의 현실적 사회개혁론 - 경(敬)·의(義)를 중심으로 -)

  • Lee, mi rim
    • The Journal of Korean Philosophical History
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    • no.39
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    • pp.29-52
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    • 2013
  • Philosophy of respect righteousness advocated by Nammyung points to what first needs to be done to drive social changes. It is consistent with the Confucianism, which views the world from within oneself. After all, developing a proper view of the society starts from looking at oneself in the right perspective. The view that personal cultivation can develop into cultivation and eventually reform of the entire society is also present in Confucius' belief that one can only be at peace after disciplining oneself and observe good manners only after perseverance. Also, one can lead the world in peace only after disciplining one's body and mind. In other words, it suggests that individuals and the community where they belong to are not independent from one another. Rather, individuals gather to create a society and righteous individuals are the basis for righteous society. These principles of social system in Confucianism can be understood as a mutual or layered division and integration. The society is not conflicted with individuals or the nation but rather perceived as moral communities to co-exist. Hence, power of execution observed in the philosophy of respect and righteousness by Nammyung is identical to power of execution applied to the society. The power of execution shows up as 'the execution of righteousness' in Nammyung's philosophy of respect and righteousness. It is also demonstrated in the acts by Nammyung's disciples when the nation was at crisis. This is what differentiates Nammyung's new-Confucianism in Joseon Dynasty from Toegye or Yulgok as he tried to deliver 'the execution of righteousness.' It does not mean that neo-Confucian scholars did not emphasize the importance of execution, but Nammyung, in particular, warned how deceitful ideological or inner-directed thinking can be since they fail to come up with concrete solutions to social issues. In summary, Nammyung's moral discipline and training based on respect and righteousness cannot be evaluated in a state of vacuum separate from social and historical aspects but under the context of enlightening virtue and social execution. This can draw the conclusion that Nammyung's execution of respect and righteousness is in the starting point of the realistic 'theory of social reform' for creating a better world.