• Journal of Mushroom
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• v.12 no.1
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• pp.24-28
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• 2014

Lentinula edodes is known by oak mushroom. It has been favored as delicious and nutritious food and the low-calorie food with a high nutritional value. It is also functional food since it contains a material well-known for its medicinal benefits. Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequate environmental control is very essential to improve the yield and quality under protected cultivation. The main objectives of the study were to investigate cultural characteristics of mycelial growth and in vitro fruiting of Lentinula edodes. The optimum culture media for mycelial growth of L. edodes were PDA and MYA. Similarly, optimum temperature was $25^{\circ}C$. Malt extract(2%) and yeast extract(0.2%) were optimum carbon and nitrogen sources. Optimal culture period was 110~120 days in sawdust medium. Mycelial growth in medium(61 mm/7 days) Quercus mongolica extract the most good. Among different five log types, highest mycelial growth and fruiting productivity were observed in Quercus variabilis sawdust(20.9%).

• Journal of Mushroom
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• v.5 no.3_4
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• pp.91-97
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• 2007

Shiitake mushroom (Lentinula edodes) is usually cultivated on the oak log. Log cultivation of this mushroom is getting difficult to get oak logs and has a weak point of its long cultivation period. Recently sawdust cultivation is getting increase. It is important to make mycelia browning on the substrate surface. This browned surface in sawdust cultivation plays an important role like as artificial bark of the oak log, which protects the other pests and suppresses water evaporation in the substrate. The period for mycelia browning is so long that the sawdust cultivation of Shiitake mushroom can not spread well into the mushroom farms. The development of methods for the rapid mycelia browning is quite required. In this article we would like to discuss about the enzymatic activities related mycelia browning and search the methods of cultivation period reduction.

• The Plant Pathology Journal
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• v.18 no.2
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• pp.74-76
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• 2002

This study unexpectedly detected a deletion in the promoter region of ras gene in two Korean strains of oak mushrooms, Lentinula edodes (Berk.). Sequencing of the promoter regions revealed that one type consisting of two strains had a 113 bp deletion in the region. The pas promoter region of Korean strains differed by 16 bases from that of the Japanese strains. Between the two types of Korean strains, except for the deleted portion, only a single site appeared to be different.

• Journal of Mushroom
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• v.16 no.1
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• pp.51-56
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• 2018

In Korea, the oak mushroom (Lentinula edodes) is highly preferred by consumers in the food industry and makes up about 97.7% of the total forest mushroom production. This indicates that the oak mushroom is an important non-timber forest product in Korea. Recently, the breeding and development of new cultivars of L. edodes have been actively initiated, and the development of molecular markers that are able to identify and discriminate the new cultivars is crucial for protecting the breeder's rights. This study was carried out to develop a cleaved amplified polymorphic sequence (CAPS) marker for the identification and discrimination of a new cultivar, Sanmaru 2ho from the 37 other oak mushroom cultivars. A single nucleotide polymorphism (SNP) was identified at the $1,803,483^{rd}$ position of scaffold2 in the genome of Sanmaru 2ho. The amplified DNA containing the SNP of Sanmaru 2ho was uniquely not cleaved by the restriction enzyme, Hha I, and thus Sanmaru 2ho was successfully distinguished from the other oak mushroom cultivars.

• Journal of Mushroom
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• v.19 no.1
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• pp.66-70
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• 2021

We monitored the occurrence of fungal species on bed-logs inoculated with oak mushroom (Lentinula edodes) and the environmental conditions of temperature and humidity in the cultivation houses during monsoon. Six fungal species, viz., Cladosporium sp., C. cladosporioides, C. anthropophilum, Pleosporales sp., Trichoderma harzianum, and Acremonium sp., wereidentified from the cultivation houses located in Jangheung, Jeonnam province. This identification was confirmed by performing nucleotide sequence analysis of the internal transcribed spacer and 28S rDNA regions. Our study presents significant findings that can help in preventing fungal damage induced by inappropriate temperature and humidity in oak mushroom cultivation houses.

• Journal of Mushroom
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• v.16 no.4
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• pp.272-278
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• 2018

Lignin degrading enzymes from Lentinula edodes have broad substrate specificities, and therefore can degrade a variety of recalcitrant compounds. In this study, the lignolytic biodegradation was investigated in five different L. edodes fungi (Chunbaegko, Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko). The fungi were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in malt extract broth medium. Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko rapidly decolorized RBBR within 7 days. The activities of manganese peroxidase (MnP) and laccase were determined in the absence and presence of lignin. Poongnyunko displayed the highest ligninolytic activity on day 7 of incubation (2,809 U/mg and 2,230 U/mg for MnP and laccase, respectively).

• The Korean Journal of Mycology
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• v.30 no.2
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• pp.170-172
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• 2002

An unrecorded Hypoxylon sp. was observed on the bed-log of oak-mushroom (Lentinula edodes) in Korea. The stroma of the fungus was formed on the bed-log (Quercus spp.) damaged by Korean squirrel (Sciurus vulgaris coreae). Perithecia were $200{\sim}400{\mu}m$, and asci containing 8 ascospores were $7{\sim}8{\times}150{\sim}230{\mu}m$ in size. Paraphysis was cylindrical shape, Ascospores were $5{\sim}9{\times}12{\sim}17.5{\mu}m$ in size, pale brown to dark brown, elliptical, and smooth. They have germination cleft and $1{\sim}2$ oil droplets.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2008.04a
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• pp.222-228
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• 2008

The chemical composition and thermal, crystal characterization of oak mushroom waste were investigated in comparison with those normal oak wood for utilization of cellulose from oak mushroom waste. The oak mushroom waste contained a higher percentage of ash, and hot water extractives than oak wood. This results indicated that the materials inside the body are easily decomposed during the oak mushroom cultivation. The lower percentage of holocellulose and a-cellulose of oak mushroom waste caused by fungal decomposition too. Whereas, the thermal decomposition behavior and crystallinity of oak mushroom waste was similar to that of normal oak wood, which indicated that the cellulose characterization of oak mushroom waste is resistant to fungal decomposition. In additionally, a degree of polymerization of oak mushroom waste must be investigate for examination of cellulose crystalline characterization, especially.

• Journal of Mushroom
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• v.18 no.3
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• pp.179-188
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• 2020

The oak mushroom (Lentinula edodes) is a popular edible mushroom that has long been cultivated and eaten throughout Northeast Asia (including Korea). Its taste and flavor are unique. Oak mushrooms are cultivated on logs and in sawdust. The cultivation period on logs is 4-5 years, while the cultivation period in sawdust is 6 months, which is rapidly driving a shift from log to sawdust based cultivation. In particular, the Sanjo 701ho cultivar of L. edodes is a mid-range to high-temperature variety that is widely known for its suitability for summer cultivation on sawdust in Korea. This study summarizes new developments and achievements in the oak mushroom industry in Korea, and details the cultivation history of Sanjo 701ho, its culturing characteristics, various demonstration tests, and the expansion of domestic varieties.

• Journal of Mushroom
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• v.16 no.2
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• pp.70-73
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• 2018

To determine the optimum amount of spent oyster mushroom substrate (SOMS) for use in cultivation of Lentinula edodes, the chemical properties of the substrate and culture conditions of Lentinula edodes were investigated. Replacing 20-50% of a sawdust substrate with SOMS yielded a C/N ratio of 62-76. In case of substrates containing SOMS, the total nitrogen and phenolic contents of were higher, whereas fructose and organic acid contents were lower than those of the control substrate. Cultivation tests showed that the 3-cycle yield of 20% SOMS treatment was 286.7 g, similar to that of the control, while 50% SOMS treatment significantly decreased the yield. In conclusion, development of oak mushroom substrate using SOMS would recycle waste products and decrease material costs.