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http://dx.doi.org/10.14480/JM.2018.16.1.51

Development of a CAPS marker for the identification of the Lentinula edodes cultivar, 'Sanmaru 2ho'  

Moon, SuYun (Department of Biology, Chungbuk National University)
Lee, Hwa-Yong (Department of Biology, Chungbuk National University)
Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science)
Koo, Chang-Duck (Department of Forest Science, Chungbuk National University)
Ryu, HoJin (Department of Biology, Chungbuk National University)
Publication Information
Journal of Mushroom / v.16, no.1, 2018 , pp. 51-56 More about this Journal
Abstract
In Korea, the oak mushroom (Lentinula edodes) is highly preferred by consumers in the food industry and makes up about 97.7% of the total forest mushroom production. This indicates that the oak mushroom is an important non-timber forest product in Korea. Recently, the breeding and development of new cultivars of L. edodes have been actively initiated, and the development of molecular markers that are able to identify and discriminate the new cultivars is crucial for protecting the breeder's rights. This study was carried out to develop a cleaved amplified polymorphic sequence (CAPS) marker for the identification and discrimination of a new cultivar, Sanmaru 2ho from the 37 other oak mushroom cultivars. A single nucleotide polymorphism (SNP) was identified at the $1,803,483^{rd}$ position of scaffold2 in the genome of Sanmaru 2ho. The amplified DNA containing the SNP of Sanmaru 2ho was uniquely not cleaved by the restriction enzyme, Hha I, and thus Sanmaru 2ho was successfully distinguished from the other oak mushroom cultivars.
Keywords
CAPS marker; Lentinula edodes; Single nucleotide polymorphism; Cultivar identification;
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1 Bak WC, Park YA, Park JH. 2013. Present situation and future of oak mushroom industry: NIFos forest policy issue 11. p. 1. Korea Forest Research Institute.
2 Bisen PS, Baghel RK, Sanodiya BS, Thakur GS, Prasad GB. 2010. Lentinus edodes: A macrofungus with pharmacological activities. Curr Med Chem 17:2419-2430.   DOI
3 Fu LZ, Zhang HY, Wu XQ, Li HB, Wei HL, Wu QQ. 2010. Wang LA. Evaluation of genetic diversity in Lentinula edodes strains using RAPD, ISSR and SRAP markers. World J Microbl Biotech 26:709-716.   DOI
4 International Mycological Association. Mycobank Database Fungal Databases, Nomenclature and Species Banks. Available online: http://www.mycobank.org/ (9 January 2018).
5 KATI. Available online: http://www.kati.net (12 January 2017). (in Korean)
6 Kaundun S, Matsumoto S. 2003. Development of CAPS markers based on three key genes of the phenylpropanoid pathway in tea, Camellia sinensis (L.) O. Kuntze, and differentiation between assamica and sinensis varieties. TAG Theoretical and Applied Genetics 106:375-383.   DOI
7 Konieczny A, Ausubel FM. 1993. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 4:403-410.   DOI
8 Korea Forest Service. 2017. The Statistical Yearbook of Forestry 2017. p. 292-331. Korea Forest Service. (in Korean)
9 Korea Rural Economic Institute. 2016. Forestry observation for Lentinula edodes, November. p. 1-6. Korea Rural Economic Institute. (in Korean)
10 Kulkarni RK. 1991. DNA Polymorphisms in Lentinula edodes, the Shiitake Mushroom. App Environ Microbiol 57:1735-1739.
11 Kunihisa M, Fukino N, Matsumoto S. 2003. Development of cleavage amplified polymorphic sequence (CAPS) markers for identification of strawberry cultivars. Euphytica 134:209-215.   DOI
12 Lee HY, Moon S, Shim D, Hong CP, Lee Y, Koo CD, Chung JW, Ryu H. 2017. Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars. Genes 8:109.   DOI
13 Li HB, Wu XQ, Peng HZ, Fu LZ, Wei HL, Wu QQ, Jin QY, Li N. 2008. New available SCAR markers: potentially useful in distinguishing a commercial strain of the superior type from other strains of Lentinula edodes in China. Appl Microbiol Biotechnol 81:303-309.   DOI
14 Liu J, Wang ZR, Li C, Bian YB, Xiao Y. 2015. Evaluating genetic diversity and constructing core collections of Chinese Lentinula edodes cultivars using ISSR and SRAP markers. J Basic Microbiol 55:749-760.   DOI
15 Liu JY, Ying ZH, Liu F, Liu XR, Xie BG. 2012. Evaluation of the use of SCAR markers for screening genetic diversity of Lentinula edodes strains. Curr Microbiol 64:317-325.   DOI
16 Korea Forest Service. 2008. Test guidelines for Lentinula edodes. p. 1-29. Korea Forest Service. (in Korean)
17 Moon S, Lee HY, Kim M, Ka, KH, Ko HG, Chung JW, Koo CD, Ryu H. 2017b. Development of CAPS Marker for Identification of Lentinula edodes Cultivars Sanmaru 1 and Chunjang 3. Kor J Mycol 14:114-120. (in Korean)
18 National Institute of Forest Science. 2016. Golden Seed Mushroom Cultivation Technology. p. 68. National Institute of Forest Science. (in Korean)
19 Moon S, Lee HY, Shim D, Kim M, Ka, KH, Ryoo R, Ko HG, Koo CD, Chung JW, Ryu H. 2017a. Development and Molecular Characterization of Novel Polymorphic Genomic DNA SSR Markers in Lentinula edodes. Mycobiology 45:105-109.   DOI
20 Moriya Y, Yamamoto K, Okada K, Iwanami H, Bessho H, Nakanishi T, Takasaki T. 2007. Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles. Plant cell reports 26:345-354.   DOI
21 Ng ML, Yap AT. 2002. Inhibition of human colon carcinoma development by lentinan from shiitake mushrooms (Lentinus edodes). J Altern Complement Med 8:581-589.   DOI
22 Qin LH, Tan Q, Chen MJ, Pan YJ. 2006. Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes. FEMS Microbiol Lett 257:112-116.   DOI
23 Reale S, Doveri S, Diaz A, Angiolillo A, Lucentini L, Pilla F, Martin A, Donini P, Lee D. 2006. SNP-based markers for discriminating olive (Olea europaea L.) cultivars. Genome 49:1193-1205.   DOI
24 Rop O, Mlcek J, Jurikova T. 2009. Beta-glucans in higher fungi and their health effects. Nutr Rev 67:624-631.   DOI
25 Royse DJ. 2014. A global perspective on the high five: Agaricus, Pleurotus, Lentinula, Auricularia & Flammulina. p. 1-6. Proceedings of the 8th International Conference on Mushroom Biology and Mushroom Products.
26 Shim D, Park SG, Kim K, Bae W, Lee GW, Ha BS, Ro HS, Kim M, Ryoo R, Rhee SK, Nou IS, Koo CD, Hong CP, Ryu HJ. 2016. Whole genome de novo sequencing and genome annotation of the world popular cultivated edible mushroom, Lentinula edodes. J Biotechnol 223:24-25.   DOI
27 Zhang R, Huang C, Zheng S, Zhang J, Ng TB, Jiang R, Zuo X, Wang H. 2007. Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers. Appl Microbiol Biotechnol 74:140-145.   DOI
28 Terashima K, Matsumoto T. 2004. Strain typing of shiitake (Lentinula edodes) cultivars by AFLP analysis, focusing on a heat-dried fruiting body. Mycoscience 45:79-82.   DOI
29 Wu X, Li H, Zhao W, Fu L, Peng H, He L, Cheng J. 2010. SCAR makers and multiplex PCR-based rapid molecular typing of Lentinula edodes strains. Curr Microbiol 61:381-389.   DOI
30 Xiao Y, Liu W, Dai Y, Fu C, Bian Y. 2010. Using SSR markers to evaluate the genetic diversity of Lentinula edodes' natural germplasm in China. World J Microbl Biotech 26:527-536.   DOI
31 Zhang Y, Molina FI. 1995. Strain typing of Lentinula edodes by random amplified polymorphic DNA assay. FEMS Microbiol Lett 131:17-20.   DOI