Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Development of a CAPS marker for the identification of the Lentinula edodes cultivar, 'Sanmaru 2ho'

표고버섯 품종 '산마루2호'를 구분할 수 있는 CAPS marker 개발

  • Moon, SuYun (Department of Biology, Chungbuk National University) ;
  • Lee, Hwa-Yong (Department of Biology, Chungbuk National University) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Koo, Chang-Duck (Department of Forest Science, Chungbuk National University) ;
  • Ryu, HoJin (Department of Biology, Chungbuk National University)
  • Received : 2018.02.27
  • Accepted : 2018.03.23
  • Published : 2018.03.31
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Abstract

In Korea, the oak mushroom (Lentinula edodes) is highly preferred by consumers in the food industry and makes up about 97.7% of the total forest mushroom production. This indicates that the oak mushroom is an important non-timber forest product in Korea. Recently, the breeding and development of new cultivars of L. edodes have been actively initiated, and the development of molecular markers that are able to identify and discriminate the new cultivars is crucial for protecting the breeder's rights. This study was carried out to develop a cleaved amplified polymorphic sequence (CAPS) marker for the identification and discrimination of a new cultivar, Sanmaru 2ho from the 37 other oak mushroom cultivars. A single nucleotide polymorphism (SNP) was identified at the $1,803,483^{rd}$ position of scaffold2 in the genome of Sanmaru 2ho. The amplified DNA containing the SNP of Sanmaru 2ho was uniquely not cleaved by the restriction enzyme, Hha I, and thus Sanmaru 2ho was successfully distinguished from the other oak mushroom cultivars.

우리나라에서 표고버섯은 소비자의 선호도가 매우 높고, 전체 임산버섯 생산량의 약 97.7%를 차지하고 있는 매우 중요한 단기소득임산물중 하나이다. 이러한 표고버섯은 최근 신품종의 개발이 활발히 이루어지고 있어, 육종가의 권리 보호를 위하여 품종을 구분할 수 있는 분자마커 개발이 요구되고 있다. 본 연구에서는 신품종 '산마루2호'를 37개의 표고버섯 품종으로부터 구분할 수 있는 CAPS 마커를 개발하였다. '산마루2호'의 유전체에서 Scaffold 2번 1803483에 위치한 단일염기 다형성(SNP)이 확인되었고, 이 SNP를 포함하여 증폭한 DNA는 제한효소 Hhal에 의하여 특이적으로 절단되지 않아 다른 표고버섯 품종들과 구분되었다.

Keywords

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