• 한국식품과학회지
• /
• 제51권4호
• /
• pp.361-368
• /
• 2019

황칠추출분말은 여성호르몬인 에스트로겐과 구조적 유사성을 가진 루틴을 다량 함유하고 있다(황칠추출분말 1g당 11.19 mg의 루틴 함유). 루틴은 식물성 여성호르몬이라고 불리는 플라보노이드 성분의 일종으로, 에스트로겐 수용체를 통한 신호전달을 증가시킨다. 특히 $ER-{\alpha}$로부터 세포내로 전달되는 MAPK/ERK 신호 전달 경로를 활성화시킴으로써, 갱년기의 대표적인 만성증상들을 개선할 수 있는 가능성이 있다고 여겨진다. 본 연구는 루틴이 다량 함유된 황칠추출분말을 폐경의 유도를 위해 난소를 절제한 쥐에 투여하여 여성갱년기 증상의 개선에 미치는 영향을 평가하였다. 그 결과 골교체율 관련 지표로서 폐경기 여성에서 높다고 알려진 오스테오칼신이 감소하는 경향성을 나타냈고, CTX는 유의적인 감소를 나타냈을 뿐만 아니라, 에스트로겐 수용체의 활성화를 증가시키는 경향성을 나타냈다. 이를 통해, 황칠추출분말은 골다공증의 예방 및 개선 효과를 나타내는 갱년기 여성 건강에 도움을 줄 수 있는 기능성 원료로서의 가능성을 보여준다.

• 경영과정보연구
• /
• 제38권3호
• /
• pp.1-19
• /
• 2019

이 연구에서는 시장의 상황에 따라 이질적으로 나타나는 유가변동성지수(Oil Volatility Index : OVX)가 주식시장에 미치는 효과를 분위수회귀모형을 이용하여 분석하였다. 특히 전체적인 주식시장뿐만 아니라 산업별로 상이하게 나타나는 효과를 분석하기 위하여 2007년 5월부터 2019년 2월까지의 종합주가지수(KOSPI)와 함께 22개 산업별 주가지수 수익률을 사용하였다. 이와 함께 유가변동성지수의 변화율이 증가하는 경우와 감소하는 경우를 구분하여 강세와 약세 시장에서 산업별 주가지수에 미치는 영향을 분석하였다. 그 결과, 각 산업별 주식시장이 약세일 때 유가변동성지수가 미치는 음의 효과가 상대적으로 강하게 나타났으며, 이러한 효과는 강세시장으로 갈수록 사라지는 것을 확인할 수 있었다. 또한 해당 산업의 주식시장이 약세일 때 유가변동성의 증가는 12개 산업에서 통계적으로 유의한 강한 음의 효과를 주는 것으로 나타났으며, 이와는 달리 강세 시장에서는 섬유의복, 기계, 서비스업에서 통계적으로 유의한 양의 효과를 주는 것으로 나타났다. 특히 강세 시장에서 유가변동성 증가가 감소하는 경우 제조업을 포함한 12개 산업에서 주가 수익률에 통계적으로 유의한 음의 효과를 주는 것으로 나타났다. 결과를 통하여 부정적인 소식에 상대적으로 더욱 민감하게 반응하는 주식시장의 특징이 약세시장에서 더욱 명확하게 나타난다는 것을 확인하였다.

• 생명과학회지
• /
• 제33권3호
• /
• pp.252-259
• /
• 2023

노화에 따른 난소 기능 저하와 성호르몬 생성 감소는 호르몬 결핍을 유발하여 여성의 갱년기를 유발한다. 여성 갱년기 증상 개선을 목적으로 에스트로겐 유사 물질이 함유된 천연물을 연구하였다. 본 연구는 난소 적출술을 받은 쥐를 대상으로 Gastrodia elata Blume 추출물(TVB-1000)의 효능을 평가하였다. 난소 적출 수술을 한뒤 7일의 회복 기간 후 TVB-1000 처리군에게 16, 40, 100 mg/kg을 12주 동안 경구 투여하였다. OVX 양성 대조군에는 17β-estradiol을 10 ㎍/kg의 용량으로 쥐의 등 부위에 피하 주사하였다. 그 결과 TVB-1000 처리군에서 심혈관질환 지표인 TG와 LDL-C가 모든 농도에서 유의하게 감소했다. LDL-C/HDLC 비는 TVB-1000 투여군 중 Medium, High군에서 유의하게 감소하였다. 또한 TVB-1000 고농도 처리군에서 복부지방 무게가 통계적으로 유의하게 감소하였다. ER- α의 발현량은 저농도 및 중농도 투여군에서 통계적 유의성을 보이며 증가하였으나 ER-β는 유의한 변화를 보이지 않았다. 실험을 통해 TVB-1000은 에스트로겐 유사 효과로 인해 갱년기 증상 중 하나인 심혈관계 질환을 예방하는 기능성 식품 소재로써 유용할 수 있음을 보여준다.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
• /
• pp.69-69
• /
• 2003

Neuroprotective strategies have been appeared to be effective in a variety of stroke models. One of the major focuses has been related to the activities of estrogen. $17\beta$-estradiol valerate(EV) has been reported to exert neuroprotective effects when administered before an ischemic insult. The purpose of this study was to determine whether EV can protect against brain injury via estrogen receptor. Chronic and acute pretreatment can reduce the ischemic damage of focal cerebral ischemia in OVX rat, indicating that EV may be a new therapeutic class of drugs to prevent neuronal damage associated with cerebral ischemia. RNAs were extracted from the hippocampus of ovariectomized female rat with or without EV. Differential gene expression profiles were revealed(Bone morphogenetic protein type 1A receptor, Protein disulphide isomerase, cytochrome bc-1 complex core P, thiol-specific antioxidant protein). RT-PCR and in situ hybridization were used to validate the relative expression pattern obtained by the cDNA array. This Study was supported by the Korea Science and Engineering Foundation(KOSEF) through the Biohealth Products Research Center(BPRC), Inje University, Korea

• Journal of Nutrition and Health
• /
• 제29권6호
• /
• pp.590-593
• /
• 1996

This study was done to evaluate the effect of dietary calcium level (a diet which met 100% or twice the calcium level in AIN-76 diet) on preventing bone loss in ovariectomized rats. Forty Sprauge-Dawley female rats(body weight 200$\pm$5g)were divided into two groups. One group were ovariecotomized (Ovx) while the others received sham operation(Sham). Thereafter, each rat group was further divided into normal calcium diet(0.52%) and high calcium diet(1.04%) subgroups. All rats were fed on experimental diet and deionized water ad libitum for 8 weeks. The total body, spine and femur bone mineral densities and bone mineral contents were measured by Dual Energy X-ray Absorptiometry, Eight weeks following operation, ovariectomized rats fed a high calcium diet had a significantly higher total bone mineral content, total bone calcium content, spine bone mineral density, spine bone mineral content and femur bone mineral content than ovariectomized rats fed control calcium diet. The correlation between dietary calcium intake level and spine bone mineral density were positive, but there was no correlation between dietary calcium intake and femur bone mineral density. The findings from the present study demonstrated that bone loss due to ovarian hormonal deficiency can be partially prevented by a high calcium diet. Futhermore, these findings support the strategy of the use of a high calcium diet in the prevention of estrogen depleted bone loss(postmenopausal osteoporosis)

• 대한한의학회지
• /
• 제21권4호
• /
• pp.9-15
• /
• 2000

Objective : The aim of this study was to investigate the neuroprotection effect of estrogen on brain atrophy following cerebral infarction. Method : All animals in this study were classified into 4 groups; ovariectomy group (OVXgroup), cerebral infarction group (INF group), combination ovariectomy and cerebral infarction group (OVX + INF group), and naturally intact group for control data (NOR group). Cerebral infarction was made by Chen's method with some modification. Ovariectomy was performed by Wayforth's method. Experimental data for each group was collected at 15 days, month, 3 months, and 6 months after starting observation. Serum $17{\beta}-estradiol(E2)$ was determined by radioimmunoassay. Brain volume was measured and calculated with image analysis. Each brain was sliced at intervals of 2mm in chamber after 30 min of freezing in refregerater. Cerebral volume was obtained by sum of volume of each slice level, which was mean $area{\;}{\times}{\;}2mm$. Results : Cerebral ischemia was found to decrease the serum concentration of $17{\beta}-{\;}estradiol(E2)$ and to inhibit the physiologically conpensatary function of the ovariectomized rats. Also we found that deprivation of estrogen have resulted in more severe cerebral atrophy followed by cerebral infarction. Conclusion : It is suggested that estrogen has a neuroprotection effect on cerebral atrophy following cerebral infarction.

• Archives of Pharmacal Research
• /
• 제27권8호
• /
• pp.867-872
• /
• 2004

Oxidative stress due to reactive oxygen species (ROS) can cause oxidative damage to cells. Cells have a number of defense mechanisms to protect themselves from the toxicity of ROS. Mitochondria are especially important in the oxidative stress as ROS have been found to be constantly generated as an endogen threat. Mitochondrial defense depends mainly on super-oxide dismutase (SOD) and glutathione peroxidase (GPx), whereas microsomal defense depends on catalase (CAT), which is an enzyme abundant in microsomes. SOD removes superoxide anions by converting them to $H_2O$$_2$, which can be rapidly converted to water by CAT and GPx. Also, GPx converts hydroperoxide (ROOH) into oxidized-glutathione (GSSG). Ovariectomized (OVX) rats are used as an oxidative stress model. An ovariectomy increased the levels of MDA, one of the end-products in the lipid peroxidative process, and decreased levels of the antioxidative enzymes； SOD, CAT and GPx. However, Chondroitin sulfate (CS) decreased the levels of MDA, but increased the levels of SOD, CAT and GPx in a dose-depen-dent manner. Moreover, inflammation and cirrhosis of liver tissue in CS- treated rats were sig-nificantly decreased. These results suggest that CS might be a potential candidate as an anti oxidative reagent.

• 대한추나의학회지
• /
• 제1권1호
• /
• pp.125-137
• /
• 2000

the aim of this study was to investigate whether osteoporosis can be advanced after cerebral infarction Of not and role of female sex hormone. all animals were classified 4 groups; group of ovariectomy (OVXgroup) group of cerebral infarction( INF group), group of combination ovariectomy and cerebral infarction(OVX + INF group), naturally intact group for control data (NOR group ). cerebral infarction was made by Chen,s method with some modification and ovariectomy was performed by Wayforth,s method. experimental data was collected at 15 days, 1 month, 3 months and 6months after starting observation. serum 17 ${{\beta}-estradiol\;(E_2)}$ was determined by radioimmunoassay, for comparision of osteoporosis formation, bone density, serum osteocalcin, Serum total calcium and phosphorus, Serum AST Concentration, Serum ALT Concentration, Creatinine Concentration were also calculated. we have found that cerebral ischemia decreases not only the serum concentration of 17 ${{\beta}-estradiol\;(E_2)}$ and inhibits but also the physiologically compensatory function of the ovariectomized rats and that the decreased estrogen concentration followed by cerebral infarction have not produced osteoporosis, regretfully.

• Journal of Nutrition and Health
• /
• 제22권2호
• /
• pp.102-107
• /
• 1989

중년기 여성들에서 체중증가로 인한 여러 가지 건강문제가 심각해지는데 폐경으로 인한 sex steroid hormone의 변화에 기인되는 것으로 생각된다. Sex steroid hormone의 부족은 체중, 특히 체지방의 축적을 가져오고 한편 뼈의 손실을 가져온다고 알려져 있다. 본 연구에서는 폐경기 후의 여성들과 유사한 조건을 만들기 위해 성숙한 암쥐를 이용하여 난소절제를 한후 장기적인 체조성의 변화를 조사하였다. 난소절제한 쥐들은 체중과 체지방의 무게가 유의적으로 증가한데 반하여 근육과 뼈의 무게는 대조군과 비교할 때 커다란 변화가 없었다. 이 연구결과는 폐경기 여성들에게 일어나는 체조성 변화에 미치는 sex steroid hormone의 영향을 조사하기 위한 실용적인 animal model로 난소절제한 쥐의 사용가능성을 암시해 주고 있다.

• Biomolecules & Therapeutics
• /
• 제8권2호
• /
• pp.147-152
• /
• 2000

The rodent uterotrophic assay is currently recommended as one of the primary in vivo assays far endocrine disrupting chemicals by the Organization for Economic Cooperation and Development (OECD) and Endocrine Disruptor Screening and Testing Advisory Committee (US EPA EDSTAC). Generally, this assay relies on the rapid increase in uterus and vagina weights when exposed to estrogenic compounds. Phthalate esters have been used extensively as a plasticizer in the manufacture of plastic products such as PVC films and medical devices. Recently, phthalate esters have been shown to induce endocrine system mediated responses. However, a flew studies have been conducted for the screening of their estrogenic activity. In this study the estrogenic activity of seven phthalate esters, butyl benzyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), di-n-butylphthalate (DBP), diethylphthalate (DEP), di-n-pentylphthalate (DPF), di-n-propylphthalate (DPrP) and dicyclohexylphthalate (DCHP), was examined in uterotrophic assay. Phthalate esters dissolved in corn oil were administered to ovariectomized (OVX) female Sprague-Dawley rats by sub-cutaneous injection for three consecutive days. fiats were sacrificed 24h after final treatment, and then uterus and vagina weights were deter mined. All phthalate esters tested in this assay did not change talc uterus and vagina weights at dosage levels up to 200 mg/kg/day treatment. These results demonstrated that phthalate esters did not exhibit estrogenic activity in vivo uterotrophic assay.