• Toxicological Research
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• 제17권
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• pp.197-199
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• 2001

Enhancement of antigen-specific IgE is a hallmark of allergic hyperresponsiveness, therefore it is necessary to adopt or develop a highly sensitive and specific assay for determination of allergen-specific IgE levels in vivo. In this presentation, we introduce an ELISA (enzyme linked immunosorbent assay) system developed to measure the levels of chicken egg ovalbumin (OVA)-specific IgE in serum. The ELISA method uses a commercially available purified rat anti-mouse IgE as a capture Ab and biotinylated OVA as a detection reagent. Avidin-peroxidase with its substrate is used for color development resulting in optical density measurement at 405 nm. The ELISA system produces a highly sensitive dose-response relation-ship between optical density levels and the dilution titer of the OVA-IgE standard serum but no cross-reaction with unrelated IgE or IgG. It is believed that the system is an Efficient tool to delineate an adjuvant effect of environmental pollutants on development of asthmatic and atopic responses.

• Journal of Microbiology and Biotechnology
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• 제23권11호
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• pp.1636-1640
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• 2013

We had found that orally administered Lactobacillus species were effective immune modulators in ovalbumin (OVA)-sensitized mice. To validate these findings, we investigated the effects of orally administered Lactobacillus brevis HY7401 in OVA-T cell receptor transgenic mice. This strain showed a tendency to induce Th1 cytokines and inhibit Th2 cytokines. All assayed isotypes of OVA-specific antibody were effectively reduced. Systemic anaphylaxis was also relatively reduced with the probiotic administration. These results reveal that L. brevis HY7401 might be useful to promote anti-allergic processes through oral administration.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2005년도 가을 학술발표논문집 Vol.32 No.2 (2)
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• pp.883-885
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• 2005

지지 벡터 기계(Support Vector Machine: SVM)를 이용한 다중부류 분류기법이 최근 활발히 연구되고 있다. SVM은 이진분류기이기 때문에 다중부류 분류를 위해서 다수의 분류기를 구성하고 이들을 효과적으로 결합하는 방법이 필요하다. 본 논문에서는 기존의 정적인 다중분류기 결합 방법과는 달리 포섭구조의 분류모델을 확률에 따라 동적으로 구성하는 방법을 제안한다. 확률적 분류기인 나이브 베이즈 분류기(NB)를 이용하여 입력된 샘플의 각 클래스에 대한 확률을 계산하고, OVA (One-Vs-All) 전략으로 구축된 다중의 SVM을 획득된 확률에 따라 포섭구조로 구성한다. 제안하는 방법은 OVA SVM에서 발생하는 중의적인 상황을 효과적으로 처리하여 고성능의 분류를 수행한다. 본 논문에서는 지문분류 문제에서 대표적인 NIST-4 지문 데이터베이스를 대상으로 제안하는 방법을 적용하여 $1.8\%$의 거부율에서 $90.8\%$의 분류율을 획득하였으며, 기존의 결합 방법인 다수결 투표(Majority vote), 승자독식(Winner-takes-all), 행동지식공간 (Behavior knowledge space), 결정템플릿(Decision template) 등보다 높은 성능을 확인하였다.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2005년도 한국컴퓨터종합학술대회 논문집 Vol.32 No.1 (B)
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• pp.622-624
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• 2005

대규모 지문인식 시스템에서 비교해야할 지문의 수를 줄이기 위해서 지문분류는 필수적인 과정이다. 최근 이진분류기인 지지 벡터 기계(Support Vector Machine: SVM)를 이용한 지문분류 기법이 많이 연구되고 있다. 본 논문에서는 다중부류 지문분류에 적합하도록 자기 구성 지도(Self-Organizing Map:SOM)를 이용하여 OVA(One-Vs-All) SVM들을 결합하는 지문분류 기법을 제안한다. SOM을 이용하여 OVA SVM들을 동적으로 결합하기 위한 결합 지도를 생성하여 지문분류 성능을 높인다. 지문분류에 있어 대표적인 NIST-4 지문 데이터베이스를 대상으로 Jain이 구축한 FingerCode 데이터베이스에 제안하는 방법을 적용하여 $1.8\%$의 거부율에서 $90.5\%$의 분류율을 획득하였으며, 기존의 결합 방법인 승자독식(Winner-takes-all)과 다수결 투표(Majority vote)보다 높은 성능을 확인하였다.

• 한국가축번식학회지
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• 제10권2호
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• pp.204-210
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• 1986

As a part of in vitro fertilization(IVF) for farm animals, IVF experiment was conducted using New Zealand white rabbits with their sperm capacitated in vivo. The effect of uterine conditions on sperm capacitation and effect of sperm concentration and fertilization media on IVF rate and implantation of in vitro fertilized ova were studied. The results obtained are summarized as follows; 1. Acrosomal reaction, noted after staining, of sperm recovered from ligated and intact uterus of capacitators was 83.0% and 65.7%, respectively. 2. IVF rate of ova inseminated with sperm from ligated uterus tended to be higher in DM or with higher concentration of sperm than in the modified F12 medium or with lower sperm concentration. Cleavage rate of fertilized ova for 48hr in DM was 31.5% for 106/ml and 30.0% for 104/ml of sperm and that in modified F12 medium was 26.0% for 106/ml and 22.3% for 104/ml of sperm. 3. Using the sperm from intact uterus, cleavage rate of fertilized ova showed same tendency as those shown with ligated uterus. The rate was 82.0% for 106/ml and 66.5% for 104/ml of sperm in DM and was 69.0% for 106/ml and 56.5% for 104/ml of sperm in the medium. 4. When normal ova up to 48hr after IVF were cultured for 4 days in either DM or modified F12 medium, ova developed to blastocyst stage showed higher rate in the groups of higher sperm concentration in the both media. The rate was 80.9% and 60.0% for 106/ml and 104/ml of sperm in DM and 91.7% and 71.4% for 106/ml and 104/ml of sperm in the modified F12 medium, respectively. 5. Rate of implantation after transfer of 4- or 8-cell embryos was 36.8%.

• 대한본초학회지
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• 제25권4호
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• pp.23-29
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• 2010

Objectives : To investigate the immunological activities, we evaluated the combination ratio of Aconiti Lateralis Radix Preparata and Glycyrrhizae Radix (AG) on murine macrophage cell line (RAW 264.7) and ovalbumin/aluminium (OVA/Alum)-immunized mice. Methods : The cellular proliferation and the production of nitric oxide were examined in a macrophage cell line, RAW 264.7 cells, in the presence of the combination ratio of Aconiti Lateralis Radix Preparata and Glycyrrhizae Radix. C57BL/6 mice were immunized intraperitonially with ovalbumin/aluminium ($100{\mu}g/200{\mu}g$) on day 1, 8, and 15. The combination ratio of Aconiti Lateralis Radix Preparata and Glycyrrhizae Radix (1 g/kg/day) was orally administrated for 3 weeks. On day 22, splenocyte and plasma were collected for mitogen-induced proliferation, lymphocyte subpopulation by flow cytometry and measurement of AST (Aspirate aminotransferase), ALT (Alanine aminotransferase), and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes). Results : Aconiti Lateralis Radix Preparata treatment had no influence on immune responses. The proliferation and NO production of macrophage and proliferation of splenocyte were increased as the higher ratio of Glycrrhizae Radix. The proliferation of splenocyte, lymphocyte subpopulation and production of antibody (total IgM, OVA-specific IgG and OVA-specific IgG1) were increased as the higher ratio of Glycrrhizae Radix on OVA-immunzed mice. Conclusions : These results suggest that the higher ratio of Glycyrrhizae Radix can increase immunological activities such as NO production in RAW264.7 cells, splenocyte proliferation and immunoglobulin production in OVA-immunized mice.

• 대한한방소아과학회지
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• 제26권1호
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• pp.1-15
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• 2012

$CheongGiGeoYangTang$ has been used for anti-allergenic purpose. However there was no experimental study about its effect. Therefore, this study was designed to investigate the anti-allergenic effect of $CheongGiGeoYangTang$. Methods Modifiability of RBL-2H3 mast cells' IL-4, IL-13 was analyzed by qRT-PCR and ELISA. Also, the suppressive effect of GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF-${\kappa}B$ p65 transcription factors was observed by western blotting. OVA-specific IgE, IL-4 and IL-13 production in ovalbumin allergy model was examined as well. Results It was showed that the RBL-2H3 mast cells treated with $CheongGiGeoYangTang$ extract(CGGYT) was significantly suppressed mRNA expression, production of IL-4 and IL-13, and prominently inhibited the expression of transcription factors including GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF-${\kappa}B$ p65 but not c-Jun. The administration of CGGYT was suppressed the amount of OVA-specific IgE, IL-4 and IL-13 in OVA/alum-sensitized mice. Conclusions We considered CGGYT would regulate the allergic inflammation as inhibition of IL-4 and IL-13 production in activated mast cells and Th2 cells.

• Journal of Microbiology and Biotechnology
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• 제18권8호
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• pp.1393-1400
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• 2008

Recent study has demonstrated an increasing prevalence of food allergy in Korean children. Specific probiotic bacteria may promote potentially anti-allergenic processes through induction of Th1-type immunity and enhance the regulatory lymphocyte. This study investigated whether orally administrated probiotics could suppress allergic responses in an ovalbumin (OVA)-induced allergy mouse model. Thus, female C3H/HeJ mice were orally sensitized with OVA and cholera toxin for 4 weeks. Lactobacillus acidophilus AD031, Bifidobacterium lactis AD011, and L. acidophilus AD031 plus B. lactis AD011 were fed to mice from 2 weeks before the sensitization. The OVA-induced mice that were not treated with probiotics had significantly increased serum levels of OVA-specific IgE and IgG1, and OVA-specific IgA in feces. However, the mice treated with probiotics suppressed production of the OVA-specific IgE, IgG1, and IgA. The level of IL-4 was significantly lower, and the levels of INF-$\gamma$ and IL-10 were significantly higher in the mice treated with probiotics than that in the non-treated mice. The groups treated with probiotics had decreased levels of degranulated mast cells, eosinophil granules, and tail scabs. These results indicate that L. acidophilus AD031 and B. lactis AD011 might be useful for the prevention of allergy.

• 한국산학기술학회논문지
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• 제13권12호
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• pp.6208-6214
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• 2012

해양미세조류 유래 독성물질에 대한 이해와 활용에 있어서 가장 중요하지만 간과되고 있는 부분은 독성물질을 검출할 수 있는 빠르고, 쉽고 경제적인 검출기술을 개발하는 것이다. 이 논문에서 우리는 삭시톡신(STX)에 대한 항체를 생산하였다. 헤모시아닌(mariculture keyhole limpet hemocyanin, mcKLH)과 오브알부민(ovalbumin, OVA)을 운반단백질로 사용하였다. 면역반응을 위해서 mcKLH-STX 결합체를 BALB/c 쥐에 복강주사하였다. 채혈 후 항-STX 항혈청을 분리하였다. 항혈청의 역가분석을 위하여 유리 STX와 OVA-STX로 코팅된 microtiter plate를 이용하여 간접 ELISA 실시하였다. 발색반응을 위한 이차항체로는 goat anti-mouse IgG-phosphatase conjugate가 사용되었다. 항-STX 항혈청은 OVA-STX와 유리 STX에 특이적으로 반응하였다. 항-STX 항혈청의 민감도는 매우 높았으며, STX를 위한 검출한계는 약 64.9 ng/kg이었다.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.373-377
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• 2002

Allergens in processed foods may place persons with food allergies at significant risk when the labels do not Provide sufficient warnings or identification of high-risk ingredients. Because egg proteins are common food allergens, this study was carried out to identify hen's egg albumin (ovalbumin, OVA) in five commercially processed foods containing egg (custayd, cookie and pasta), and chicken meat (sausage and meatball) by immunological methods using commercially produced murine monoclonal immunoglobulin G (M-IgG), immunoblotting and enzyme linked immunosorbent assay (ELISA). Sample buffer with chelating and reducing agents was prepared and used for the preparation of the protein fractions from the foods. Most bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profile (5~15% gradient gel) presented at 75 kDa below. OVA (43 kDa) in the sample lanes could not be visually observed on the gel. However, OVA in solutions prepared from custard and cookie could be detected by M-IgG, but were not detected in sausage and pasta. OVA in all samples could be quantitatively determined by the equation obtained from the standard curve by ELISA. Cookie and custard containing egg white and egg, respectively, contained very high concentrations of OVA. OVA in the other products were present in relatively low concentrations, but sufficiently high to pose possible risk of allergy, ELISA is a very sensitive and precise method for the identification and quantification of allergens in food products including allergy-inducible materials.