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http://dx.doi.org/10.5762/KAIS.2012.13.12.6208

Production of antibodies for saxitoxin analysis and sensitivity analysis of anti-saxitoxin antiserum  

Chang, Man (Korea Institute Ocean Science & Technology)
Lee, Gunsup (Korea Institute Ocean Science & Technology)
Moh, Sang Hyun (Department of Biological Science, Mokpo National University)
Shin, Kyoungsoon (Korea Institute Ocean Science & Technology)
Auh, Chung-Kyoon (Anti-aging Research Institute of BIO-FD&C Co., Ltd.)
Lee, Taek-Kyun (Korea Institute Ocean Science & Technology)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.13, no.12, 2012 , pp. 6208-6214 More about this Journal
Abstract
The most essential but missing components to understand and use toxic substances from marine microalgae are developing the fast, easy and economical determining technology for detecting it. In this paper we produced the antibodies against saxitoxin (STX). Mariculture keyhole limpet hemocyanin (mcKLH) and ovalbumin (OVA) were used as carrier proteins. mcKLH-STX conjugates were injected into the peritonial cavity of BALB/c mouse for immunization. After bleeding from mouse, anti-STX antiserum was isolated. Indirect enzyme-linked immunosorbent assays (ELISA) was performed to determine antiserum titer using the microtiter plate coated with free STX and OVA-STX. A goat anti-mouse IgG-phosphatase conjugate was used as secondary antibody to enable chromogenic reaction. Reactions of anti-STX antiserum were very specific on the OVA-STX and free STX. Sensitivity of anti-STX antiserum on STX was very high and STX detection limit was to be 64.9 ng/kg for indirect ELISA.
Keywords
Saxitoxin; Immunization; Antiserum; ELISA;
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