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http://dx.doi.org/10.7778/jpkm.2012.26.1.001

Suppressive Effect of CheongGiGeoYangTang on Allergic Inflammation of RBL-2H3 Mast Cells and OVA/alum-sensitized Mice  

Lee, Hye-Lim (Department of Pediatrics, College of Oriental Medicine, Daejeon University)
Han, Jae-Kyung (Department of Pediatrics, College of Oriental Medicine, Daejeon University)
Kim, Yun-Hee (Department of Pediatrics, College of Oriental Medicine, Daejeon University)
Publication Information
The Journal of Pediatrics of Korean Medicine / v.26, no.1, 2012 , pp. 1-15 More about this Journal
Abstract
$CheongGiGeoYangTang$ has been used for anti-allergenic purpose. However there was no experimental study about its effect. Therefore, this study was designed to investigate the anti-allergenic effect of $CheongGiGeoYangTang$. Methods Modifiability of RBL-2H3 mast cells' IL-4, IL-13 was analyzed by qRT-PCR and ELISA. Also, the suppressive effect of GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF-${\kappa}B$ p65 transcription factors was observed by western blotting. OVA-specific IgE, IL-4 and IL-13 production in ovalbumin allergy model was examined as well. Results It was showed that the RBL-2H3 mast cells treated with $CheongGiGeoYangTang$ extract(CGGYT) was significantly suppressed mRNA expression, production of IL-4 and IL-13, and prominently inhibited the expression of transcription factors including GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF-${\kappa}B$ p65 but not c-Jun. The administration of CGGYT was suppressed the amount of OVA-specific IgE, IL-4 and IL-13 in OVA/alum-sensitized mice. Conclusions We considered CGGYT would regulate the allergic inflammation as inhibition of IL-4 and IL-13 production in activated mast cells and Th2 cells.
Keywords
CheongGiGeoYangTang(淸肌祛痒湯); RBL-2H3 mast cell; OVA/alum-sensitized mice; IL-4; IL-13; Anti-allergic;
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