• Korean Journal of Heritage: History & Science
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• v.38
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• pp.359-385
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• 2005

Red ocher, red lead or cinnabar has been as red colorant for ages. Cinnabar of the red pigments has been highly regarded as a valuable ingredient because it represent a symbol of exorcising and a haute image. It was used as a pigment of painting and mural painting, bowl, clothes, rock writing, gravestone, etc. It is powder which dissolves in perilla oil or glue before using. Because it is high-priced, the use of cinnabar may be limited to the privileged class. Therefore, red ocher or red lead was used instead of cinnabar. "Gongsagyunmunrok" demonstrated that government official's gravestonea has been painted red by two colorants in the period of the Goryeo dynasty. However, cinnabar may be used to paint gravestones for the first time in the period of the Three States because it has been transmitted since the times. This study discuss the results obtained from an analysis of the pigments used on the red pigments of the Stone Monuments. The results can be briefly summarized as below; First, the microcrystalline structures seen on the surface section of analyzed pigments, samples of which were taken from various parts of red pigments show that different sizes and shapes of pigment particle. Second, a result of the analysis on the composition and structure of the pigments shows that the main components in their composition are : Red pigments - Red lead($Pb_3O_4$), Cinnabar(HgS) and Hematite($Fe_2O_3$) White pigments - Calcite($CaCO_3$) Especially, we knew that red Stone Monuments were found to be natural mineral pigments, which were used as a singular or a mixture.

• Journal of Korean Medicine for Obesity Research
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• v.18 no.2
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• pp.106-114
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• 2018

Objectives: We investigated about the microbial properties and changes in the efficacy of the Codonopsis lanceolata (CL) by natural fermentation. Methods: CL was fermented for four weeks in a well-ventilated place with 2.5% salt. pH, total sugar, total polyphenol, and total flavonoid were measured to determine fermentation characteristics according to fermentation period and salt treatment. Polymerase chain reaction denaturing gradient gel electrophoresis and random amplification of polymorphic DNA-polymerase chain reaction were carried out for microbial analysis during fermentation. In addition, HepG2 cell was cultured to check the lipid accumulation through oil red O staining and the glucose uptake was analyzed by measuring the 2-NBDG at C2C12 cell. Results: The pH level and the total sugar decreased with the CL fermentation. Total polyphenol and flavonoid increased after CL fermentation. It was confirmed that Leuconostoc mesenteroides were maintained continuously during fermentation. In the salt treatment CL, there was a sharp increase in Rahnella aquatilis. Lactobacillus plantarum matrix was observed in fermented CL. In addition, Lactococcus lactis, Weissella koreensis, R. aquatilis, L. plantarum, Leu. mesenteroides have been added to the salt treatment. Glucose uptake were significantly increased after fermentation with salt for four weeks. Lipid accumulation in the HepG2 cells was observed that there was difference (P<0.01) between free fatty acid group (100%) and decreased 4 weeks after fermentation (90.38%) at $800{\mu}g/mL$. Conclusions: Total polyphenol and flavonoid were increased after CL fermentation. Especially, percentage of the glucose uptake and lipid accumulation inhibition increased in CL fermentation with salt. It is expected that fermentation of salt treated CL will be more effective in diabetes and fatty liver.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.4
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• pp.590-596
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• 2019

Uses of fossil fuels like coal and oil increases with industrial development, and problems like abnormal climate come up as greenhouse gas increases. Accordingly, studies are actively conducted on eco-friendly renewable energy as a replacement for the main resources, and especially, wood pellets with high thermal efficiency are in the limelight as an alternative fuel in thermal power stations and gas boilers. However, despite a constant increase in their usage, few studies are conducted on their risks like fire and spontaneous combustion. Thus, this study found the auto-ignition temperature and critical ignition temperature of wood pellets with a change in flow rate in a thermostatic bath, using a sample vessel with 20 cm in length, 20 cm in height and 14 cm in thickness to predict their ignition characteristics. Consequently, at the flow rate of 0 NL/min, as the core temperature of the sample increased to higher than the ambient temperature, they ignited at $153^{\circ}C$, when the critical ignition temperature was $152.5^{\circ}C$. At the flow rates of 0.5 NL/min and 1.0 NL/min, it was $149.5^{\circ}C$, and at the flow rate of 1.5 NL/min, it was $147.5^{\circ}C$. Consequently, at the same storage, the more the flow rate, the lower the critical ignition temperature became.

• Journal of Life Science
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• v.29 no.2
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• pp.147-151
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• 2019

Soybean [Glycine max (L.) Merr.] is grown worldwide for its high protein and oil content. Anthocyanins from black soybean seed coats are known to have many pharmaceutical effects. Soybean cultivars with large seed sizes and black seed coats are needed by soybean farmers. However, antinutritional factors, like protein, stachyose, and Kunitz trypsin inhibitor (KTI) exist in raw mature soybeans. Genetic elimination or reduction of these components is needed in soybean breeding. The objective of this research was to develop new a soybean strain with black seed coats and green cotyledons that was KTI protein free and low in stachyose. Six parents were used. The presence or absence of KTI protein was detected using the Western blot technique. The content of stachyose in mature seeds was detected using HPLC. One new strain was selected from 11 $F_2$ plants with black seed coats and green cotyledons that lacked KTI protein. The new strain had black seed coats and green cotyledons and was KTI protein free and low in stachyose. The plant height of the new strain was 66 cm, and its 100-seed weight was 28.4 g. The stachyose content of the new strain was 2.59 g/kg. The new strain developed in this research will be used to develop new cultivars that are KTI protein free and low in stachyose.

• Korean Journal of Food Science and Technology
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• v.51 no.3
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• pp.272-277
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• 2019

The effects of medium-chain enriched diacylglycerol (MCE-DAG) oil on hepatic cholesterol homeostasis were investigated. HepG2 hepatocytes were treated with either 0.5, 1.0, or $1.5{\mu}g/mL$ of MCE-DAG for 48 h. There was no evidence of cytotoxicity by MCE-DAG up to $1.5{\mu}g/mL$. The level of proteins for cholesterol uptake including CLATHRIN and LDL receptor increased by MCE-DAG in a dose-dependent manner (p<0.05). Furthermore, proprotein convertase subtilisin/kexin type 9, an inhibitor of LDLR, was dose-dependently diminished (p<0.05), indicating cholesterol clearance raised. MCE-DAG significantly increased 3-hydroxy-3-methylglutaryl-coenzyme A reductase and acetyl-CoA acetyltransferase2 (p<0.05), required for cholesterol synthesis, and their transcriptional regulator sterol regulatory element-binding protein2 (p<0.05). These findings suggest that given conditions of prolonged sterol fasting in the current study activated both hepatic cholesterol synthesis and clearance by MCE-DAG. However, total intracellular level of cholesterol was not altered by MCE-DAG. Taken together, MCE-DAG has the potential to prevent hypercholesterolemia by increasing hepatic cholesterol uptake without affecting intracellular cholesterol level.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.7
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• pp.599-606
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• 2019

Most facilities that manufacture products made from the hazardous materials operate at high temperatures and pressures. Therefore, there is a risk of fire explosion. In particular, an explosion accident is a major risk factor for facilities with hazardous materials, such as oil, chemical, and gas. Propylene is often used in sites producing basic raw materials and synthetic materials by addition polymerization at petrochemical plants. To prevent an explosion in the business using propylene, the explosion range with the oxygen concentration was calculated according to the changes in temperature and pressure using an inert gas, carbon dioxide. In these measurements, the temperature was $25^{\circ}C$, $100^{\circ}C$, and $200^{\circ}C$ and the amount of carbon dioxide in the container was $1.0kgf/cm^2.G$, $1.5kgf/cm^2.G$, $2.0kgf/cm^2.G$, and $2.5kgf/cm^2.G$. The explosion limit was related to temperature, pressure, and oxygen concentration. The minimum oxygen concentration for an explosion decreased with increasing temperature and pressure. The range of explosion narrowed with decreasing oxygen concentration. In addition, no explosion occurred at concentrations below the minimum oxygen concentration, even with steam and an ignition source of propylene.

• Journal of Food Hygiene and Safety
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• v.34 no.2
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• pp.135-139
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• 2019

The objective of this research was to compare loop-mediated isothermal amplification (LAMP) with real-time polymerase chain reaction (PCR) for the rapid detection of pathogens in foods. In this study, the limits of detection (LODs) for Salmonella Typhimurium, Listeria monocytogenes, and Cronobacter sakazakii were evaluated in various foods. Among 11 samples tested for S. Typhimurium, LAMP and real-time PCR had the same LODs in beef and duck meat whereas real-time PCR was more sensitive than the LAMP in 8 samples. However, S. Typhimurium in chocolate samples was not detected by real-time PCR. The sensitivity of real-time PCR was high in all samples inoculated with L. monocytogenes and C. sakazakii whereas LAMP was more sensitive than real-time PCR in oil-rich foods. Therefore, LAMP can be shown as an easrer, more convenient method, as well as effective analytical method for testing difficult samples when employed in PCR.

• Journal of Food Hygiene and Safety
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• v.34 no.2
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• pp.212-218
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• 2019

The process of establishing domestic standards on hazardous substances in food safety regulations requires harmonization with standards from the Codex Alimentarius Commission (CAC). For this purpose, food classification by the CAC (Codex Classification of Foods and Animal Feeds) also needs to be clearly understood. Therefore, this paper aimed to introduce the Codex Classification on cereal grains, nuts/seeds and herbs/spices because revisions of the Codex were completed in 2017 for cereal grains and in 2018 for nuts/seeds and herbs/spices. The revised Codex Classification on those foods is briefly summarized as follows. Cereal grains in the domestic food classification by the Ministry of Food and Drug Safety, Korea (MFDS) corresponds to the Codex Group 020 cereal grains with six subgroups. The MFDS's nuts and seeds classification corresponds to three groups in the Codex, namely, Group 022 (tree nuts with no subgroups), Group 023 (oilseeds and oilfruits with 5 subgroups), and Group 024 (seeds for beverages and sweets with no subgroups). The food commodities of herbs and spices are included in two Codex groups, Group 027 (with 3 subgroups) and Group 028 (with 9 subgroups). The number of Codex commodity codes assigned to food commodities was 27 for Group 020, 32 for Group 022, 46 for Group 023, 4 for Group 024, 127 for Group 027 and 138 for Group 028. In between the Codex Classification and the MFDS's classification, some differences are shown. For example, the MFDS did not create a subgroup under groups of cereal grains and herbs. The MFDS classified peanuts into the nut group, though a separate group for oilseeds is present, while the Codex classified peanuts into the oilseed and oilfruit group. In addition, there is also a separate group of "plants, others" present in the MFDS's classification. Therefore, care is needed in using the Codex Classification.

• Korean Journal of Breeding Science
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• v.49 no.3
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• pp.224-229
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• 2017

A new peanut variety 'Daan'(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2014. This was developed from the cross between 'Iksan 31' with Virginia typed short stem and 'Dakwang' with Shinpung-typed larger grain. 'Daan' which is a Shinpung plant type had 13 branches per plant and its length of main stem was 44cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 34 pods per plant, 127 g of 100-seed-weight and 75% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety showed resistant to early leaf spot and had more resistant to late leaf spot, stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years 'Daan' was more productive than reference variety by 16% with 5.00 MT/ha for grain production.

• Journal of the Korean Society for Environmental Technology
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• v.19 no.6
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• pp.576-585
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• 2018

Systematic management during the whole life cycle from construction to operation and maintenance is very important for the seven underground pipelines (waterworks, sewerage, electricity, telecommunications, gas, heating, oil including waterworks and sewerage). Especially, it is the construction process that affects the whole life cycle of underground buried pipeline. In order to construct a new city or to maintain different underground pipes, it is always necessary to dig the ground and carry out construction and related work. There is a possibility that secondary and tertiary breaks frequently occur in the pipeline construction process after the piping constructed first in this process. To solve this problem, a system is needed which can monitor damage in real time. However, the supply of electric power for continuous operation of the system is limited according to the environment of underground buried pipelines, so it is necessary to develop a stable electric power supply system using natural energy rather than existing electric power. In this study, we developed a system that can operate the pipeline monitoring system for long time (24 hours and 15 days) using natural energy using wind and solar light.