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http://dx.doi.org/10.13103/JFHS.2019.34.2.135

Comparison of Loop-Mediated Isothermal Amplification and Real-Time PCR for the Rapid Detection of Salmonella Typhimurium, Listeria monocytogenes and Cronobacter sakazakii Artificially Inoculated in Foods

Kim, Jin-Hee (Department of Food and Nutrition, Kookmin University)
Oh, Se-Wook (Department of Food and Nutrition, Kookmin University)

Publication Information

Journal of Food Hygiene and Safety / v.34, no.2, 2019 , pp. 135-139 More about this Journal

Abstract

The objective of this research was to compare loop-mediated isothermal amplification (LAMP) with real-time polymerase chain reaction (PCR) for the rapid detection of pathogens in foods. In this study, the limits of detection (LODs) for Salmonella Typhimurium, Listeria monocytogenes, and Cronobacter sakazakii were evaluated in various foods. Among 11 samples tested for S. Typhimurium, LAMP and real-time PCR had the same LODs in beef and duck meat whereas real-time PCR was more sensitive than the LAMP in 8 samples. However, S. Typhimurium in chocolate samples was not detected by real-time PCR. The sensitivity of real-time PCR was high in all samples inoculated with L. monocytogenes and C. sakazakii whereas LAMP was more sensitive than real-time PCR in oil-rich foods. Therefore, LAMP can be shown as an easrer, more convenient method, as well as effective analytical method for testing difficult samples when employed in PCR.

Keywords

3M molecular detection system; Validation; Real-time PCR;

Citations & Related Records

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