• 대한수의사회지
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• 제30권3호
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• pp.149-161
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• 1994

광견병(rabies; OIE List B 058)과 관련, 국제수역사무국 (OIE; Office deInternational Epizooties)에서 제공한 최근의 발생정보를 중심으로 역학동향과 주요국가별 광견병 관리대책을 검토하였다. 최근에 수집된 광견병 해외발생 역학정보 자료는 92/93년도 보고자료이므로 실제적으로는 91/92년도의 발생실태로 보면된다. * 자료 : Animal Health Yearbook (OIE/FAO/WHO) International Zoo-sanitary Code (OIE) HANDISTATUS; Computer Data Base (IICA)

• 한국동물위생학회지
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• 제35권4호
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• pp.263-268
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• 2012

From the end of July 2012, several cases of abortion have been happened at the Korean indigenous cattle farm with 124 heads in Chungbuk province, Korea. Serological tests such as Rose-bengal test (RBT) and standard tube agglutination test (STAT) have been performed according to the standard official protocols of bovine brucellosis and 41 cattle turned out to be brucellosis-positive simultaneously. To find out the main factors of brucellosis outbreaks and spreads, additional serological, etiological and molecular investigation were applied. Totally, 11 B. abortus were isolated from 10 cattle's specimens including lymph-nodes and/or testis, and drinking water in cowhouse. In genotyping by multi-locus VNTR assay (MLVA) using 17 loci markers, the present B. abortus isolates were shown all the same pattern, D1 genotype, which has been reported in Gyeonggi and Gangwon province, Korea. These results suggest that the input of brucellosis might come from neighboring farms directly or indirectly, even if by unknown factor and expansion within farm would accelerate by materials related with aborting cows.

• 대한수의학회지
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• 제54권4호
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• pp.197-201
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• 2014

In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.

• 월간양계
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• 제52권8호
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• pp.112-115
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• 2020

농림축산검역본부(본부장 박봉균, 이하 검역본부)는 지난 6월 16일 세계동물보건기구(OIE)에서 조류인플루엔자 OIE 표준실험실 인증을 받았다. 아시아 최초 8개의 OIE 표준실험실을 보유하면서 이번 계기로 조류인플루엔자 진단·연구 및 방역분야에서 국제적 신인도 확보는 물론 아시아 지역에서 선도기관이 될 것으로 기대된다. 검역본부 조류인플루엔자연구진단과 이명헌 과장을 만나 그간 활동과 OIE 표준실험실 인증 획득의 의미와 향후 일정 등 앞으로 활동 계획에 대해 인터뷰했다.

• 한국동물위생학회지
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• 제35권4호
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• pp.269-273
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• 2012

A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

• 대한수의학회지
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• 제55권2호
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• pp.105-110
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• 2015

A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.

• 대한수의사회지
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• 제45권2호
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• pp.164-168
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• 2009

• 월간낙농육우
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• 제27권4호통권300호
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• pp.198-203
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• 2007

최근 OIE(국제수역사무국)가 미국, 캐나다에 광우병 위험을 통제할 수 있는 국가의 등급(2등급 : 30개월령이 지난 소의 광우병 위험물질 및 머리뼈.등뼈 등에서 단순 분리한 살코기는 교역불가)을 내렸다. OIE는 오는 5월(프랑스 파리) 이를 최종 확정할 예정으로, 이 같은 OIE 질병위원회의 분류결과를 업은 미국은 더욱 거세게 쇠고기 시장의 전면개방을 요구할 것으로 예상된다. OIE 총회 이후 우리나라와 별도의 협상을 하지 않겠다는 미국의 입장에 우리나라로서는 별도의 검역기준을 마련할 예정이라지만 근본적 대책으로서는 요원하기만 하다. 이 같은 상황을 계기로 월간낙농육우는 국내축산물 유통동향 외에도 미국 및 세계 육류시장의 동향과 전망을 소개할 예정이니, 많은 관심을 바란다.

• Journal of Microbiology and Biotechnology
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• 제20권12호
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• pp.1750-1755
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• 2010

The MLVA assay is known to have a high ability to identify and discriminate Brucella species, so that it can be used as an epidemiological tool to discriminate Brucella isolates originating from restricted geographic sources. In this study, the genetic profiles of 38 B. abortus isolates from humans were analyzed and compared with genotypes from animal isolates in South Korea. As a result, it was found that they did not show high genetic diversity and were compacted. They were clustered together with animal isolates, showing a significant correlation to regional distributions. With its ability to prove a significant genetic correlation among B. abortus isolates from animals and humans in South Korea, the MLVA assay could be utilized as part of a program to control and eradicate brucellosis, one of the major zoonoses. This study represents the first data of genetic correlation of B. abortus isolates from humans and animals in South Korea.

• 월간양계
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• 제39권1호통권447호
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• pp.107-109
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• 2007

본고는 일본에서 AI로 인해 2004년에 이어 2006년 또 다시 540만수를 대량 살처분함에 따라 감염이 되지 않은 대단위 농장의 피해를 최소화하고 보다 효율적인 예방대책을 모색하기 위하여 일본양계협회, 일본수의학회, 일본계란생산자협회가 2006년 8월 9일(水) 동경 오꾸라호텔에 서 공동 주최한‘조류인플루엔자에 대한 일본의 방역대책’에 대해 국제수의사무국(OIE) 명예고문이며 일본AI방역대책에 각종 제안과 조언을 하고 있는 오자와(小洋義傳) 박사가 발표한 내용을 방역당국과 농가들에 참고자료로 활용코자 발췌·번역·게재한 것이다. 이 내용을 발표한 오자와 박사는바이러스, 수의역학을 전공하고 국제식량농업기구(FAO), 국제수역사무국(OIE), OIE아시아, 대서양지역 대표를 거친 가축전염병대책에 국제적 권위를 인정받고 있다