Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Genetic Comparison of Brucella abortus Isolates from Animals and Humans by Using the MLVA Assay

  • Her, Moon (OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Department, National Veterinary Research and Quarantine Service (NVRQS)) ;
  • Kang, Sung-Il (OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Department, National Veterinary Research and Quarantine Service (NVRQS)) ;
  • Kim, Jong-Wan (OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Department, National Veterinary Research and Quarantine Service (NVRQS)) ;
  • Kim, Ji-Yeon (OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Department, National Veterinary Research and Quarantine Service (NVRQS)) ;
  • Hwang, In-Yeong (OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Department, National Veterinary Research and Quarantine Service (NVRQS)) ;
  • Jung, Suk-Chan (OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Department, National Veterinary Research and Quarantine Service (NVRQS)) ;
  • Park, Sang-Hee (Division of Zoonoses, Center for Immunology and Pathology, National Institute of Health, Korea Centers for Disease Control and Prevention (KCDC)) ;
  • Park, Mi-Yeoun (Division of Zoonoses, Center for Immunology and Pathology, National Institute of Health, Korea Centers for Disease Control and Prevention (KCDC)) ;
  • Yoo, Han-Sang (Department of Infectious Diseases, College of Veterinary Medicine, Brain Korea 21 for Veterinary Science, KRF Zoonotic Disease Priority Research Institute, Seoul National University)
  • Received : 2010.05.27
  • Accepted : 2010.08.16
  • Published : 2010.12.28
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Abstract

The MLVA assay is known to have a high ability to identify and discriminate Brucella species, so that it can be used as an epidemiological tool to discriminate Brucella isolates originating from restricted geographic sources. In this study, the genetic profiles of 38 B. abortus isolates from humans were analyzed and compared with genotypes from animal isolates in South Korea. As a result, it was found that they did not show high genetic diversity and were compacted. They were clustered together with animal isolates, showing a significant correlation to regional distributions. With its ability to prove a significant genetic correlation among B. abortus isolates from animals and humans in South Korea, the MLVA assay could be utilized as part of a program to control and eradicate brucellosis, one of the major zoonoses. This study represents the first data of genetic correlation of B. abortus isolates from humans and animals in South Korea.

Keywords

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