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http://dx.doi.org/10.7853/kjvs.2012.35.4.269

Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis  

Sung, So-Ra (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kim, Ji-Yeon (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Her, Moon (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Lee, Kichan (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Gu, Jeong-Hui (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kang, Sung-Il (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Lee, Hyang-Keun (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kim, Suk-Mi (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Jung, Suk-Chan (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Publication Information
Korean Journal of Veterinary Service / v.35, no.4, 2012 , pp. 269-273 More about this Journal
Abstract
A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.
Keywords
Brucellosis; Serological test; STAT; FPA; ELISA;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 농림수산식품부. 2009. 결핵병 및 브루셀라병 방역실시요령. 제 2009-147호.
2 농림수산식품부. 2011. 살처분 가축 등에 대한 보상금 등 지급요령. 제 2011-24호.
3 Alton GG, Jones LM, Pietz DE. 1975. Laboratory techniques in brucellosis. pp. 7-80. 2nd ed. WHO Monograph series.
4 Bae JH, Kim SE, Jo SR, Jeong EH, Jang EH, Kwon HN, Park DY, Lee KC. 2010. Extraction and verification of highly immunologenic antigen for diagnosis of bovine brucellosis. Korean J Vet Serv 33: 135-141.   과학기술학회마을
5 Gall D, Nielsen K. 2004. Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison. Rev Sci Tech Off Int Epiz 23: 989-1002.
6 George WB. 1995. Handbook of zoonoses. pp. 9-39. 2nd ed. CRC press, London.
7 Godfroid J, Saegerman C, Wellemans V, Walravens K, Letesson JJ, Tibor A, Mc Millan A, Spencer S, Sanna M, Bakker D, Pouillot R, Garin-Bastuji B. 2002. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet Microbiol 90: 461-477.   DOI   ScienceOn
8 Hur J, Kakoman I, Jeong JM, Lee HJ, Baek BK. 2007. Comparison of a new ELISA with other serodiagnostic tests for bovine brucellosis. Korean J Vet Serv 30: 385-391.
9 Kim KH, Hwan AS, Park YH, Kim DS. 1982. Comaprison of six serological methods for the diagnosis of bovine brucellosis. Korean J Vet Res 22: 149-153.
10 Lambert G, Amerault TE. 1962. Comparative study of three serological tests for detecting the response in cattle to virulent Brucella abortus. Am J Vet Res 23: 529-533.
11 MacMillan AP, Greiser-Wilke I, Moennig V, Mathias LA. 1990. A competition enzyme immunoassay for brucellosis diagnosis. Dtsch Tierarztl Wochenschr 97: 83-85.
12 Morgan WJB, Mackinnon DJ, Gill KPW, Gower SGM, Norris PIW. 1978. Brucellosis diagnosis: standard laboratory techniques. pp. 1-53. 2nd ed. Ministry of Agriculture, Fisheries and Food, London.
13 Nielsen K, Kelly L, Gall D, Nicoletti P, Kelly W. 1995. Improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis. Vet Immunol Immunopathol 46: 285-291.   DOI   ScienceOn
14 Munoz PM, Marin CM, Monreal D, Gonzalez D, Garin-Bastuji B, Diaz R, Mainar-Jaime RC, Moriyón I, Blasco JM. 2005. Efficacy of several serological tests and antigens for the diagnosis of bovine brucellosis in the presence of false positive serological results due to Yersinia enterocolitica O:9. Clin Diagn Lab Immunol 12: 141-151
15 Nielsen K. 2002. Diagnosis of brucellosis by serology. Vet Microbiol 90: 447-459.   DOI   ScienceOn
16 Nielsen K, Kelly L, Gall D, Balsevicius S, Bosse J, Kelly W, Nicoletti P. 1996. Comparison of enzyme immunoassays for the diagnosis of bovine brucellosis. Prev Vet Med 26: 17-32.   DOI   ScienceOn
17 Nielsen K, Smith P, Yu W, Nicoletti P, Elzer P, Robles C, Bermudez R, Renteria T, Moreno F, Ruiz A, Massengill C, Muenks Q, Jurgersen G, Tollersrud T, Samartino L, Conde S, Forbes L, Gall D, Perez B, Rojas X, Minas A. 2005. Towards single screening tests for brucellosis. Rev Sci Tech Off Int Epiz 24: 1027-1038.   DOI
18 OIE. 2008. Bovine brucellosis. pp. 624-659. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). 6th ed. Office International des Epizooties.
19 Quinn PJ, Carter ME, Markey B, Carter GR. 1994. Clinical veterinary microbiology. pp. 261-267. Esevier limited, London.
20 Stack JA, Perrett LL, Brew SD, MacMillan AP. 1999. Competitive ELISA for bovine brucellosis suitable for testing poor quality samples. Vet Rec 145: 735-736.   DOI
21 Timoney JF, Gillespie JH, Scott FW, Barlough JF. 1988. Hagan Bruner's microbiology and infectious diseases of domestic animals. pp. 135-152. 8th ed. Cornell University Press, Ithaca and London.
22 Wright PF, Tounkara, Lelenta M, Jeggo MH. 1997. International reference standards: antibody standards for the indirect enzyme-linked immunosorbent assay. Rev Sci Tech 3: 824-832.
23 Weynants V, Gilson D, Cloeckaert A, Tibor A, Denoel PA, Godfroid F, Limet JM, Letesson JJ. 1997. Characterization of smooth lipopolysaccharide and O polysaccharides of Brucella species by competition binding assays with monoclonal antibodies. Infect Immun 65: 1939-1943.
24 WHO. 1986. Joint food and agriculture organization of the United nations/world health organization expert committee on brucellosis. pp. 740. 6th ed. World Health Organization Technical Report Series 740, Geneva.
25 Wright PF, Nisson E, Van Rooij EM, Lelenta M, Jeggo MH. 1993. Standardisation and validation of enzyme-linked immunosorbent assay techniques for the detection of antibody in infectious disease diagnosis. Rev Sci Tech 12: 435-450.   DOI