• Applied Biological Chemistry
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• v.47 no.4
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• pp.422-425
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• 2004

The flower of Erigeron annuus L. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three compounds were isolated through the repeated silica gel, ODS column and high performance liquid chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\alpha}-amyrenone,\;{\alpha}-amyrin\;and\;{\beta}-amyrin$. These three compounds were isolated for the first time from the flower of Erigeron annuus L.

• Analytical Science and Technology
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• v.11 no.6
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• pp.436-439
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• 1998

Ginseng saponins were analysed using reversed-phase high performance liquid chromatography with several columns. The optimum conditions were as following : reverse phase column; Novapak $C_{18}$ ODS column ($3.9mm{\times}150mm$, $5{\mu}m$), acetonitrile/water binary mobile phase gradient controller system, solvent flow rate; 1.5 mL/min, and UV (203 nm) detector. The complete separation of ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf and $Rg_1$ was achieved within 50 min. The regression coefficients of the calibration curves for seven ginsenosides were 0.98~0.99.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.51-56
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• 2003

The angiotensin I converting enzyme (ACE) has an important role in the maintenance of blood pressure. The ACE inhibitory activities of foods have recently been studied. We tried to isolate ACE inhibitory peptides from the Flavourzyme (FZ), Pescalase (PE), and Thermolysine (TH) protease digests of corn gluten, which was restricted to the use the source of food for digestion problem. The FZ, PE, TH/PE protease hydrolyzed corn gluten and the inhibitory activities of the hydrolyzates for ACE were measured. Major fractions were isolated from the digests using ODS chromatography after treating with ethanol in step gradient. The ACE inhibitors were further purified by Bio-Gel P-2 column and reverse phase HPLC. Five inhibitory peptides were isolated. Their amino acids were sequenced as LPF ($IC_{50}$ = 40$\mu$M), GPP ($IC_{50}$ = 17.6$\mu$M), PNPY ($IC_{50}$ = 30.7$\mu$M), SPPPFYL ($IC_{50}$ = 63 $\mu$M), and SQPP ($IC_{50}$ = 17.2$\mu$M).

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.30 no.3
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• pp.201-210
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• 2017

Objectives : To report the patient suspected chronic arsenic intoxication after Korean medicine treatment. Methods : One patient with highly suspected chronic arsenic intoxication was selected. He was suspected to be chronic arsenic intoxication because symptoms and hair heavy metals test. Acupuncture was applied for twice a day and herbal medicine(Pyeonghyeol-eum) was administered in 28 days. Evaluation method applied to xeroderma, pruritus, psoriasis were overall dry skin score(ODS), visual analogue scale(VAS), pruritus grading system(PGS). Results : Pyeonghyeol-eum, acupuncture and the other Korean medicine treatments improved xeroderma, pruritis, psoriasis after 28 days of treatment. ODS decreased from 3 to 2. VAS of Pruritus decreased from 8 to 1. PGS decreased from 15 to 4. Adverse effects were not reported. Conclusions : This study shows that the Korean medicine treatment was effective in impoving symptoms of chronic arsenic intoxication. But the additional study will be conducted for revealing dermatitis caused by chronic arsenic intoxication.

• Korean Journal of Food Science and Technology
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• v.29 no.3
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• pp.412-416
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• 1997

Seven brands of commercial apple juice in Korea obtained from local stores were analyzed for patulin. Sep-Pak silica cartridge purification of the extract by ethyl acetate offered a good separation of patulin from other components. Patulin was determined by reverse phase liquid chromatography using a J'sphere $4\;{\mu}m$ ODS-H80 column with an ultraviolet detector set at 274 nm. Patulin concentrations in four samples ranged from 4 to $20\;{\mu}g/L$ and the other three samples from 30 to $45\;{\mu}g/L$. The limit of detection was $3\;{\mu}g/L$, and the recovery was $80{\sim}90%$ at the contamination level of $3.78{\sim}125.9\;{\mu}g/L$.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.385-390
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• 2009

To investigate the cellular growth traits of a photosynthetic green algae, Chlorella pyrenoidosa, several tests upon a culture temperature, a culture time, the influence of nutrient and the intensity of illumination were executed. Using growth chamber, some optimal conditions for the culture of algae were as follows: The culture temperature was about $28^{\circ}C$, the culture time about 4 days, and the cellular growth of algae was in proportioned to the concentration of nutrient such as nutrient broth. And the more the intensity of illumination was increased, the more the algal cell showed good growth. And then, the activity of enzyme degrading acetaldehyde was also studied using HPLC from the same strain. This enzyme was dependent on $\beta$-$NAD^+$. And showed its optimal pH around on 9.0, and also its optimal temperature around at $40^{\circ}C$. The operational conditions of HPLC were as follows: Column, ODS-Hypersil ; mobile phase, 50% (v/v) acetonitrile.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.164-169
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• 2004

In the course of our screening for biocontrol agent (BCA) against Streptomyces scabiei and S. turgidiscabies causing potato scab using 5,000 actinomtcete isolates, 9 antagonistic strains were selected as BCA candidates through in vitro and in vivo assay. An antagonistic strain, A020645 was highly resistant to some pesticides and antibiotics such as dazomet and mancozeb and showed high control value in vivo. Two bioactive compounds (compound A, B) were purified by anion exchange chromatography, solid phase (ODS) extraction, TLC and reverse phase HPLC. Their chemical structures are now thought to be nucleoside derivative as determined by $^1H-NMR$ data analysis. Their full chemical structures would be elucidated through $^{13}C-NMR$, HMQC and HMBC analyses. Further studies will be focused on fitness in soil and formulation of the BCA candidates.

• Applied Biological Chemistry
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• v.50 no.1
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• pp.53-56
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• 2007

The aerial parts of Artemisia princeps PAMPANINI were extracted with 80% aqueous MeOH and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2$O, successively. From the EtOAc fraction, five compounds were isolated through the repeated silica gel and ODS column chromatog-raphies. They were determined as friedelin (1), ${\beta}$-amyrin (2), ${\beta}$-amyrin acetate (3), camphanediol (4) and hispidulin (5) on the basis of spectral data, respectively.

• Applied Biological Chemistry
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• v.51 no.1
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• pp.60-64
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• 2008

The aerial parts of Spathiphyllum cannifolium were extracted with 80% aqueous MeOH and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three compounds were isolated through the repeated silica gel, ODS, Sephadex LH-20 column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as stigmasterol (1), $(2S)1-O-linolenoyl-2-0-myristoyl-3-0-{\beta}-D-galactopyranosyl-sn-glycerol$ (2) and stigmasterol glucoside (3). They were the first to be isolated from Spathiphyllum cannifolium.

• Korean Journal of Food Science and Technology
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• v.28 no.1
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• pp.184-189
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• 1996

A scavenger of superoxide anion radical which causes oxygen toxicity was isolated from Capsella bursa-pastoris, and its physicochemical properties were investigated. The scavenger was isolated and purified by solvent fractionation and liquid column chromatographies (Amberlite XAD-2, Sephadex LH-20, Bio gel P-2, ODS (silica gel with 100% octadecyl silanization)). An active compound of 0.25 g was finally isolated by Fast Protein Liquid chromatography (FPLC) from 100 g ethanol extract of Capsella bursa-pastoris. A 50% decrease of superoxide anion radical was obtained with the scavenger compound of 0.58 g. The compound was assumed to be a phenolic glycoside from its physicochemical properties.