Cellular Growth Traits and Detection of Acetaldehyde Dehydrogenase from Chlorella pyrenoidosa

To investigate the cellular growth traits of a photosynthetic green algae, Chlorella pyrenoidosa, several tests upon a culture temperature, a culture time, the influence of nutrient and the intensity of illumination were executed. Using growth chamber, some optimal conditions for the culture of algae were as follows: The culture temperature was about $28^{\circ}C$, the culture time about 4 days, and the cellular growth of algae was in proportioned to the concentration of nutrient such as nutrient broth. And the more the intensity of illumination was increased, the more the algal cell showed good growth. And then, the activity of enzyme degrading acetaldehyde was also studied using HPLC from the same strain. This enzyme was dependent on $\beta$-$NAD^+$. And showed its optimal pH around on 9.0, and also its optimal temperature around at $40^{\circ}C$. The operational conditions of HPLC were as follows: Column, ODS-Hypersil ; mobile phase, 50% (v/v) acetonitrile.

Chlorella pyrenoidosa의 생장 특성 및 동일 균주로부터 Acetaldehyde Dehydrogenase의 활성 검출

광합성 담수 녹조류인 Chlorella pyrenoidosa의 최적 생장 조건을 알기 위해 배양 온도, 시간, 영양물질의 영향 및 조도 등을 조사하였다. Growth chamber를 사용하여 알아본 가장 적절한 조건은 온도 $28^{\circ}C$에서 4일간 배양했을 때이며 배지에 첨가된 타 영양물질의 농도가 높을수록 활발한 광합성을 하면서 생장하였고 조도(Lux)가 크면 클 수록 잘 자랐다. 또한 동일 균주로부터 acetaldehyde를 분해하는 효소의 활성을 살펴보았는데 이 효소는 $\beta$-$NAD^+$를 조효소로 하는 탈수소효소였으며, ODS-Hypersil column과 50%(v/v) acetonitrile을 이동상으로 한 HPLC로 분석한 결과 pH 9.0, 온도 $40^{\circ}C$ 부근에서 최대 효소 활성을 보여주었다.