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Screening of Antagonistic Actinomycetes for Potato Scab Control and Isolation of Antibiotic Compound  

Lee, Hyang-Burm (Korea Research Institute of Bioscience & Biotechnology)
Cho, Jong-Wun (Korea Research Institute of Bioscience & Biotechnology)
Lim, Chi-Hwan (College of Agricultural Life Science, Chungnam National University)
Kim, Chang-Jin (Korea Research Institute of Bioscience & Biotechnology)
Publication Information
Applied Biological Chemistry / v.47, no.2, 2004 , pp. 164-169 More about this Journal
Abstract
In the course of our screening for biocontrol agent (BCA) against Streptomyces scabiei and S. turgidiscabies causing potato scab using 5,000 actinomtcete isolates, 9 antagonistic strains were selected as BCA candidates through in vitro and in vivo assay. An antagonistic strain, A020645 was highly resistant to some pesticides and antibiotics such as dazomet and mancozeb and showed high control value in vivo. Two bioactive compounds (compound A, B) were purified by anion exchange chromatography, solid phase (ODS) extraction, TLC and reverse phase HPLC. Their chemical structures are now thought to be nucleoside derivative as determined by $^1H-NMR$ data analysis. Their full chemical structures would be elucidated through $^{13}C-NMR$, HMQC and HMBC analyses. Further studies will be focused on fitness in soil and formulation of the BCA candidates.
Keywords
potato; potato common scab; BCA; Streptomyces scabiei; Streptomyces turgidiscabies; bioactive compounds;
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