Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 28 Issue 1
- /
- Pages.184-189
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- 1996
- /
- 0367-6293(pISSN)
Purification and Physicochemical Properties of Superoxide Anion Radical Scavenger from Capsella bursa-pastoris
냉이(Capsella bursa-pastoris)로부터 Superoxide Anion Radical 소거물질의 정제 및 이화학적 성질
- Kwak, Jae-Hyock (Department of Food Technology, Korea University) ;
- Kweon, Mee-Hyang (Institute of Biotechnology, Korea University) ;
-
Ra, Kyung-Soo
(Department of Food and Nutrition, Taegu Technical Junior College)
;
-
Sung, Ha-Chin
(Department of Genetic Engineering, Korea University)
;
- Yang, Han-Chul (Department of Food Technology, Korea University)
- 곽재혁 (고려대학교 식품공학과) ;
- 권미향 (고려대학교 생물공학연구소) ;
-
나경수
(대구공업전문대학 식품영양과)
;
-
성하진
(고려대학교 유전공학과)
;
- 양한철 (고려대학교 식품공학과)
- Published : 1996.02.28
Abstract
A scavenger of superoxide anion radical which causes oxygen toxicity was isolated from Capsella bursa-pastoris, and its physicochemical properties were investigated. The scavenger was isolated and purified by solvent fractionation and liquid column chromatographies (Amberlite XAD-2, Sephadex LH-20, Bio gel P-2, ODS (silica gel with 100% octadecyl silanization)). An active compound of 0.25 g was finally isolated by Fast Protein Liquid chromatography (FPLC) from 100 g ethanol extract of Capsella bursa-pastoris. A 50% decrease of superoxide anion radical was obtained with the scavenger compound of 0.58 g. The compound was assumed to be a phenolic glycoside from its physicochemical properties.
냉이(Capsella bursa-pastoris)로부터 산소독성에 영향을 미치는 superoxide anion radical에 대하여 소거작용을 하는 물질을 분리하고 이 물질의 이화학적 성질을 검토하였다. 냉이를 에탄올로 추출한 후 용매분획과 각종 column chromatographies (Amberlite XAD-2, Sephadex LH-20, Bio gel P-2, ODS)를 통해 정제하였고, 최종적으로 FPLC (Fast protein liquid chromatography)를 사용하여 활성의 단일물질을 얻었다. 냉이 에탄올추출물 100 g으로부터 0.25 g의 정제물질을 얻었으며, superoxide anion radical을 50% 소거하는 농도