• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.44 no.2
• /
• pp.103-110
• /
• 2018

To develop a new functional agent for cosmetics, we investigated the anti-aging activities in fibroblasts using sweroside isolated from Nymphoides indica. The anti-aging effect of sweroside was validated in CCD-986sk cells. Results showed that sweroside inhibited expressions of UVB-induced ROS and increased pro-collagen. Furthermore, sweroside had inhibited MMP-1 expression. Taken together, the results suggested that sweroside has considerable potential as a new cosmetics ingredient with an anti-aging effect.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.6 no.1
• /
• pp.41-50
• /
• 2003

The feasibility as floricultural crops and water garden plant materials of Korean native water plants was investigated. Propagation type, growing and flowering time were observed for development as water garden, interior aquarium plant and water purification materials. Flowering time of the water plant was 7 species in May, 28 species in June, 49 species in July, 55 species in August, 47 species in September, and 17 species in October. Beautiful flowering water plants were Nymphaeaceae, Nymphoides peltata, Nymphoides indica, Monochoria korsakowii, Iris pseudacorus, Iris laevigata, and etc. Ornamental leafy water plants were Ceratopteris thalictroides, Ludwigia ovalis, Myriophyllum verticillatim, Limnophila sessiliflora, Blyxa aubertii, Blyxa echinosperma, Vallisneria asiatica, Hydrilla verticillata and Eleocharis acicularis etc. Isoetes japonica, Isoetes coreana and Isoetes sinensis were propagated by spore. Blyxa aubertii, Blyxa echinosperma, Myriophyllum verticillatim, Nuphar japonicum, Nelumbo nucifera, Ottelia alismoides, Sagittaria aginashi, Trapa japonica, and Trapa natans were propagated by seed. Persicaria amphibia, Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Oenanthe javanica, Potamogeton crispus, Hydrilla verticillata and Acorus calamus were propagated by division. And Vallisneria asiatica, Hydrilla verticillata and Phragmites japonica were propagated by runner. Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Limnophila sessilifera were propagated by adventitious bud. Ceratopteris thalictroides was propagated by leaf cutting. The 35 genera, 68 species of water plants were available for horticultural use. The 45 species such as Iris laevigata, Eleocharis acicularis, Menyanthes trifoliata, Nymphaea minima, Nuphar pumilum, Nymphoides coreana, Nymphoides peltata, Nymphoides indica, Nymphaea tetragona (water lily), and Typha latifolia could be use for water garden plant. The 21 species such as Limnophila sessilifera, Vallisneria asiatica, Ceratophyllum demersum and Hydrilla verticillata available for indoor aquarium. The 19 species such as Ottelia alismoides, Oenanthe javanica, Limnophila sessilifera and Blyxa echinosperma could be culture in container. The 27 species such as Trapa japonica, Trapa incisa, Phramites commuris (reed), Phragmites japonica, and Zizania latifolia were usable for water purification plant materials.

• Journal of Applied Biological Chemistry
• /
• v.61 no.4
• /
• pp.321-325
• /
• 2018

In this study, we evaluated the possibility of wrinkle-reducing functional cosmetic material of isoquercitrin (IQC) isolated from Nymphoides indica (NP) by measuring the efficacy of ROS inhibition, procollagen synthesis induction, and matrix metalloproteinase (MMP)-1 inhibition. As a result, ROS tended to be inhibited in a concentration dependent manner, and the procollagen protein synthesis rate was increased by 70% ($5{\mu}g/mL$) and the MMP-1 protein was decreased by 41% ($5{\mu}g/mL$) compared with the control. This was suggested the ability of IQC to induce the procollagen synthesis and to reduce the ROS and MMP-1 production. Therefore, it is considered that IQC isolated from NP has enough value to be a functional cosmetic material.

• Journal of Applied Biological Chemistry
• /
• v.60 no.4
• /
• pp.327-332
• /
• 2017

In this study, the whitening activity of Nymphoides indica extract in B16F10 cells were measured. Inhibition rate of tyrosinase from mushroom was 42% at $1,000{\mu}g/mL$. And inhibition of tyrosinase and melanin biosynthesis in B16F10 cells were 26 and 25% at $5{\mu}g/mL$, respectively. The expression levels of cAMP and protein kinase A (PKA), which are higher levels of melanin-related factors, were found to be decreased in a dose-dependent manner. In addition, the expression rate of protein and mRNA of tyrosinase, tyrosinase related protein 1 (TRP1), tyrosinase related protein 2 (TRP2) and microphthalmia associated transcription factor (MITF). In this study, it was confirmed that the N. indica extract effectively inhibited the activity of tyrosinase, TRP1, TRP2 and MITF as well as the activity of PKA by effectively inhibiting cAMP. Therefore, it was confirmed that the N. indica extract has high value as a functional material.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2005.09b
• /
• pp.1162-1163
• /
• 2005

• Journal of Plant Biotechnology
• /
• v.34 no.1
• /
• pp.7-10
• /
• 2007

Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2Ms medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. In this study, low concentration of 2,4-D had a stimulative role in embryogenic callus formation, whereas BA showed inhibitory role in callus formation. In comparison to floral stem, leaf explants showed low frequency of embryogenic callus formation. The highest frequency of embryogenic callus formation from leaf explants was 9.5% when leaf explants were cultured onto 1/2MS medium supplemented with 0.3 mg/L of 2,4-D. The plant regeneration system of Nymphoides indica established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.45 no.3
• /
• pp.307-317
• /
• 2019

To develop sustainable new natural anti-aging ingredients from Korean native plants, we investigated the cultivation potential of Nymphoides indica using the eco-friendly aquaponics system, and tested the anti-aging effects from N. indica extracts. N. indica could be grown in aquaponics system using floating leaved deep water culture method, and propagated through rhizome propagation. It was confirmed that the nitrate ($80{\mu}g/mL$), potassium ($63.5{\mu}g/mL$) and water temperature ($25^{\circ}C$) greatly affected the cultivation of the N. indica. In addition, synergistic effects were found when two major components (3,7-di-O-methylquercetin-4'-O-${\beta}$-glucoside & sweroside) were present at more than about $5{\mu}g/mL$. The extract had a significant effect on the recovery of skin cells damaged by environmental pollutant such as $benzo[{\alpha}]pyrene$, ammonium nitrate, formaldehyde. It also suppressed $PGE_2$, $TNF-{\alpha}$ and COX-2, and inhibited the production of MMP-1. Taken together, the results suggested that the standardized extracts of N. indica cultivated in the aquaponics has considerable potential as a new cosmetics ingredient with an anti-aging effect.

• The Korean Journal of Ecology
• /
• v.25 no.5
• /
• pp.305-313
• /
• 2002

The physico-chemical characteristics of water and sediment, and structures of vegetation of the vascular hydrophytes were investigated in the West Nakdong River. Water quality was eutrophic according to the mean values and the ranges of water properties such as pH, DO, BOD, chlorophyll a, total nitrogen and phosphate, and other nutrients. A few cases were hypereutrophic for chlorophyll a level in summer. Soil reaction was weak acid. Composition of sediment was mainly sand except in SI(Sinan chideung) of which was mainly clay, and SU(Suan chideung) of which was mainly silt. Flora of vascular hydrophyte had 26 species and 1 variety comprising 16 families. Trapa japonica was dominant species in the sites of DU(Dunchido), GA(Garak chideung) and SU. Nymphoides peltata and Hydrocharis dubia dominated in DA and SI, respectively. Species diversity and evenness were relatively high in SI and SU but dominance was high in DA. After June, water lettuce(Pistia stratiotes) and water hyacinth(Eichhornia crassipes) were flowed from tributary to the river. Standing crop of macrohydrophytes was high in DA from April to August, but it showed maximum standing crop (445g·dw/㎡) in DU after disturbance by explosive growth of exotic plants in October. In comparison with those in 1985, total productivities in DU and GA decreased to 33.5%, and the reduction ratio of dominant species, Trapa japonica was 56.7%. Najas marina, N. minor, Myriophyllum spicatum and Nymphoides indica have disappeared ever since the Nakdong barrage was constructed in the Nakdong river. They were divided into three groups (GA-SU-DU, DA, SI) by cluster analysis. Introduction of the exotic species in this river caused decreasing of endemic plants including endangered species Euryale ferox and rare species Hydocharis dubia, and food plants for waterfowl such as Trapa japonica, Vallisneria asiatica and Potamogeton crispus.

• Journal of Wetlands Research
• /
• v.20 no.3
• /
• pp.281-293
• /
• 2018

Wetlands of Dongbaekdongsan were classified into two types of pond and vernal pool. 11 plant communities are established there such as Nymphoides indica community, Sparganium stoloniferum Community, Persicaria hastato-auriculata community, Scirpus triangulatus community, Triadenum japonica community, Juncus papillosus community, Deinostema violacea community, Centipeda minima community, Apocynum sibiricum community, Isachne globosa community, and Ulmus parvifolia community. Nymphoides indica community is established in a pond located on the entrance of the Dongbaekdongsan and Persicaria hastatoauriculata community appears on edge of the pond. Sparganium stoloniferum community is formed in nearby pond with relatively shallow water and Persicaria hastatoauriculata community is established on margin of the pond. Vernal pools had been used for drinking water source and for livestock in some cases in the past. Scirpus triangulatus community is established in the most wet site as the center of the pools and Juncus papillosus community - Deinostema violacea community - Triadenum japonica community, and Apocynum sibiricum community appear in a concentric circle pattern as far away there. Ulmus parvifolia community is established in a dry vernal pool. This is the first study on vernal pool carried out in Korea. Therefore, most vegetation types are new ones different from existing studies on wetland.

• Journal of Wetlands Research
• /
• v.7 no.4
• /
• pp.67-80
• /
• 2005

Vegetation structure of the vascular plants was investigated from April 2003 to August 2003 in Hwangjeong wetland around Geumho River, Yeongcheon-si, Gyeongsangbuk-do, Korea. Actual vegetation of Hwangjeong wetland largely can be classified by the floristic composition and the physiognomy into 12 communities; Ambrosia artemisiifolia var. elatior, Humulus japonicus, Miscanthus sacchariflorus, Phragmites japonica, Zizania latifolia-Miscanthus sacchariflorus, Zizania latifolia-Nymphoides peltata, Miscanthus sacchariflorus-Phragmites japonica, Phragmites communis-Phragmites japonica, Phragmites japonica-Salix gracilistyla, Salix koreensis-Salix glandulosa, Salix nipponica-Salix koreensis, and Phragmites japonica-Zizania latifolia. Among them, the distribution area of the Phragmites japonica community was the largest as 49.46 ha(11.03%). The dominant vegetation type was Phragmites japonica community and Ambrosia artemisiifolia var. elatior community based on the phytosociological method, and Phragmites japonica community was classified into two subcommunities; Nymphoides peltata subcommunity and Salix glandulosa subcommunity. Differential species of Phragmites japonica community were Phragmites japonica, Miscanthus sacchariflorus, Persicaria thunbergii, Oenanthe javanica, Leersia oryzoides var. japonica, and Rorippa indica; differential species of Ambrosia artemisiifolia var. elatior community were Ambrosia artemisiifolia var. elatior, Setaria glauca, Commelina communis, Cyperus orthostachyus, Digitaria sanguinalis, Xanthium strumarium, Erigeron annuus, Erigeron canadensis, Kummerowia striata, Trifolium repens, and Medicago sativa; differential species of Nymphoides peltata subcommunity were Nymphoides peltata, Zizania latifolia, Scirpus tabernaemontani, and Eleocharis mamillata var. cyclocarpa; differential species of Salix glandulosa subcommunity were Salix glandulosa, Salix koreensis, and Salix gracilistyla. It was expected that Hwangjeong wetland is worthy of conservation contributed purifying water pollution, giving habitats of many lifes, and providing beautiful scenes of Geumho River.