Browse > Article
http://dx.doi.org/10.3839/jabc.2017.051

Inhibitory effect of Nymphoides indica extract on α-MSH induced melanin synthesis  

Kim, Dong-Hee (Applied Product Development Team, National Development Institute of Korean Medicine)
Kim, You-Ah (Skin Science Research Institute, Kolmar Korea Co., Ltd.)
Yu, Jae-Myo (Applied Product Development Team, National Development Institute of Korean Medicine)
Park, Chae-Bin (Skin Science Research Institute, Kolmar Korea Co., Ltd.)
Park, Byoung-Jun (Skin Science Research Institute, Kolmar Korea Co., Ltd.)
Park, Tae-Soon (Applied Product Development Team, National Development Institute of Korean Medicine)
Publication Information
Journal of Applied Biological Chemistry / v.60, no.4, 2017 , pp. 327-332 More about this Journal
Abstract
In this study, the whitening activity of Nymphoides indica extract in B16F10 cells were measured. Inhibition rate of tyrosinase from mushroom was 42% at $1,000{\mu}g/mL$. And inhibition of tyrosinase and melanin biosynthesis in B16F10 cells were 26 and 25% at $5{\mu}g/mL$, respectively. The expression levels of cAMP and protein kinase A (PKA), which are higher levels of melanin-related factors, were found to be decreased in a dose-dependent manner. In addition, the expression rate of protein and mRNA of tyrosinase, tyrosinase related protein 1 (TRP1), tyrosinase related protein 2 (TRP2) and microphthalmia associated transcription factor (MITF). In this study, it was confirmed that the N. indica extract effectively inhibited the activity of tyrosinase, TRP1, TRP2 and MITF as well as the activity of PKA by effectively inhibiting cAMP. Therefore, it was confirmed that the N. indica extract has high value as a functional material.
Keywords
B16F10; Melanin; ${\alpha}$-melanocyte stimulating hormone; Nymphoides indica; Tyrosinase;
Citations & Related Records
Times Cited By KSCI : 12  (Citation Analysis)
연도 인용수 순위
1 Cho HR, Kang KA, Bhuiyan MH, Oh MS, Lee MH, Kim YJ (2011) Antimelanogenic effect of Pini Nodi Lignum extract in HM3KO melanoma cells. Mol cell Toxico 7(2): 135-139   DOI
2 Chun HJ, Choi WH, Baek SH, Woo WH (2002) Effect of quercetin on melanogenesis in melan-a melanocyte cells. Kor J Pharmaco 33: 245-251
3 Eipper BA, Mains RE (1980) Structure and biosynthesis of pro-adrenocorticotropin/ endorphin and related peptides. Endocr Rev 1(1): 1-27   DOI
4 Gismondi A, Canuti L, Impei S, Di Marco G, Kenzo M, Colizzi V, Canini A (2013) Antioxidant extracts of African medicinal plants induce cell cycle arrest and differentiation in B16F10 melanoma cells. Int J Oncol 43(3): 956-964   DOI
5 Han GS, Kim DS, Woo WH, Mun YJ (2010) Inhibitory effect of fructus Ligustri Lucidi on tyrosinase and MITF expressions. Kor J Ori Physiol Pathol 24(2): 296-301
6 Hearing VJ (1999) Biochemical control of melanogenesis and melanosomal organization. J Investig Dermatol Symp Proc 4(1): 24-28   DOI
7 Hwang J, Lee BM (2007) Inhibitory effects of plant extracts on tyrosinase, LDOPA oxidation, and melanin synthesis. J Toxicol Environ Health A 70(5): 393-407   DOI
8 Jang JY, Kim HN, Kim YR, Kim BW, Choi YH, Choi BT (2010) Studies of Inhibitory Mechanism on Melanogenesis by Partially Purified Asiasari radix in $\alpha$-MSH Stimulated B16F10 Melanoma Cells. J Life Sci 20(11): 1617-1624   DOI
9 Jang TW, Park JH (2017) Evaluation of Whitening Activities of Flower and Fruit of Sophora japonica L. Kor J Plant Res 30(1): 22-28   DOI
10 Woo HS, Choi HJ, Han HS, Park JH, Son JH, An BJ, Son GM, Choi C (2009) Isolation of polyphenol from Green Tea by HPLC and its physiological activities. Kor J Food Sci Technol 35(6): 1199-1203
11 Yagi A, Kanbara T, Morinobu N (1986) The effect of tyrosinase inhibition for aloe. Planta medica 3981: 517-519
12 Yamaguchi Y, Hearing YJ (2009) Physiological factors that regulate skin pigmentation. Biofactors 35(2): 193-199   DOI
13 Kim AY, Yim NH, Im MJ, Jung YP, Liang C, Cho WK, Ma JY (2013) Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells. BMC Complement Altern Med 13(1): 214   DOI
14 Seo SY, Sharma VK, Sharma N (2003) Mushroom tyrosinase: Recent prospects. J Agric Food Chem 51(10): 2837-2853   DOI
15 Thody AJ, Ridley K, Penny RJ, Chalmers R, Fisher C, Shuster S (1983) MSH peptides are present in mammalian skin. Peptides 4(6): 813-816   DOI
16 Kang MK, Lee YE, Woo WH, Mun YJ (2014) Commelina communis Ledeb inhibitis melanin synthesis in alpha-MSH-stimulated B16F10 cells. J Physiol Pathol Korean Med 28: 506-511
17 Yang ES, Hwang JS, Choi HJ, Hong RH, Kang SM (2008) The effect of genistein on melanin synthesis and in vivo whitening. Kor J Microbiol Biotechnol 36(1): 72-81
18 Kim DS, Sung BG, Lee JC, Lee BK, Woo WH, Lim KS (2011) Inhibitory Effect of Belamcandae Rhizoma on the Melanogenesis in MSHstimulated B16F10 cells. J Kor Orient Med Ophthalmol Otolaryngol Dermatol 24(1): 25-35
19 Kim HS (2016) The anti-melanogenic effect of Geranium kraneru Extract. Korean J Food Sci Technol 48(1): 72-76   DOI
20 Lee SY, Yoo DH, Joo DH, Jo HS, Lee JY (2015) Inhibitory Efficacy of Smilax china L. on MITF, TRP-1, TRP-2, Tyrosinase Protein and mRNA Expression in Melanoma Cell (B16F10) Microbiol Biotechnol Lett 44(1): 1-8   DOI
21 Lee YS, Choi BD, Joo EY, Shin SR, Kim NW (2009) Antioxidative activities and tyrosinase inhibition ability in various extracts of the Vitex rotundifolia seeds. Kor J Food Preserv 16(1): 101-108
22 No JK, Soung DY, Kim YJ, Shim KH, Jun YS, Rhee SH, Yokozawa T, Chung HY (1999) Inhibition of tyrosinase by green tea components. J Life Sci 65(21): 241-246
23 Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, Hearing VJ, Lozano JA, Garcia-Borron JC (1994) A new enzymatic function in the melanogenic pathway. The 5, 6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J Biol Chem 269(27): 17993-18000
24 Oh MJ, Min SR, Liu JR, Kim SW (2007) Plant regeneration from floral stem cultures of Nymphides indica (L.) O. Kuntxe. Via somatic embryogenesis. J plant Biotechnol 34(1): 7-10   DOI
25 Park SY, Jin ML, Kim YH, Kim YH, Lee SJ (2011) Aromatic-turmerone inhibits $\alpha$-MSH and IBMX-induced melanogenesis by inactivating CREB and MITF signaling pathways. Arch Dermatol Res 303(10): 737-744   DOI
26 Park SY, Kim YH, Kim YH, Park GT, Lee SJ (2010) Beta-carboline alkaloids harmaline and harmalol induce melanogenesis through p38 mitogenactivated protein kinase in B16F10 mouse melanoma cells. BMB Reports 43(12): 824-829   DOI
27 Ryu IS, Park SJ, Mun YJ, Ko JS, Shin KD, Lee JC, Woo HW, Lim KS (2007) Inhibitory effects of Flowers of Lespedeza bicolor on Tyrosinase Activity and Melanin Synthesis. Kor J Ori Physiol Pathol 21(5): 1142-1147
28 Jeong YO, Park NB, Gwak JS, Jeong SJ (2010) Yasaenghwadogam. Pureunhaengbok, Seoul
29 Busca R, Ballotti R (2000) Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res 13(2): 60-69   DOI
30 Chan YY, Kim KH, Cheah SH (2011) Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. J Ethnopharmacol 137(3): 1183-1188   DOI
31 Chang MS, Kim HM, Yang WM, Kim DR, Park EH, Ko EB, Choi MJ, Kim HY, Oh JH, Shim KJ, Yoon JW, Park SK (2007) Inhibitory effects of Nelumbo nucifera on tyrosinase activity and melanogenesis in clone M-3 melanocyte cells. Kor J Herbol 22(4): 87-94