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http://dx.doi.org/10.5010/JPB.2007.34.1.007

Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis  

Oh, Myung-Jin (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
Min, Sung-Ran (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Liu, Jang-Ryol (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Suk-Weon (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Plant Biotechnology / v.34, no.1, 2007 , pp. 7-10 More about this Journal
Abstract
Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2Ms medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. In this study, low concentration of 2,4-D had a stimulative role in embryogenic callus formation, whereas BA showed inhibitory role in callus formation. In comparison to floral stem, leaf explants showed low frequency of embryogenic callus formation. The highest frequency of embryogenic callus formation from leaf explants was 9.5% when leaf explants were cultured onto 1/2MS medium supplemented with 0.3 mg/L of 2,4-D. The plant regeneration system of Nymphoides indica established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.
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