• Journal of Microbiology
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• v.34 no.2
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• pp.117-123
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• 1996

Restriction-polymorphic patterns of nuclear-ITS were examined for the genetic relationships among 12 bisidiomycetous mushrooms to Aphyllophorales and Agaricales. The taxonomic affinity of Ganoderma species with the family Polyporaceae also was examined. With 13 restriction endonucleases, 159 restriction characters were generated form the 12 species examined. UPGMA and neighbor-joining analyses separated the 12 species into two genetically distinct groups that correspond to orders (Agaricales and Aphyllophorales) where each species is included. This result indicates that there is clear genetic demarcation between Agaricales and Aphyllophorales. Dendrograms constructed by several data analyses showed that even though Ganoderma species are somewhat in intermediate taxonomic position between the Polyporaceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

• ALGAE
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• v.20 no.2
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• pp.91-97
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• 2005

The brown algal family Chordariaceae sensu lato is a focus of taxonomy because recent studies suggest a broad concept of the family, including genera formerly classified in the Dictyosiphonales. Using morphology, plastid rbcL and nrDNA ITS sequences, we evaluated relationships of the monotyic genus Soranthera (S. ulvoidea), which has been classified in the Punctariaceae. The species occurs in Bering Sea and Aleutian Islands, Alaska to Baja California. Thalli are globose to lobed, hollow, 3-5 cm in diameter, and covered with evenly distributed sori. However, two forms within the species are recognized: f. ulvoidea for globose forms and f. difformis for lobed forms. Plastid rbcL and nuclear ITS region sequences were newly determined in samples of S. ulvoidea from the Pacific coast of the North America. We found little variations in the ITS sequences among samples of S. ulvoidea from five different locations and in the rbcL region from two different locations. These results do not support previous classification of f. ulvoidea and f. difformis within the species. All analyses of our rbcL sequence dataset show that Soranthera was placed in the Chordariaceae s.l., but more related to Botrytella than Punctaria and Asperococcus.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.11a
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• pp.84-91
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• 2003

Flow-Induced Vibration of steam generator tubes may result in fretting wear damage at the tube-to-support locations. KSNP(Korean Standard Nuclear Power plant) steam generators experienced fretting wear in the upper part of U-bend above the central cavity region of steam generators. This region has conditions susceptible to the flow-induced vibration, such as high flow velocity, high void fraction, and longer unsupported span. To improve its performance, APR1400 steam generator is designed with additional supports in this region to reduce unsupported span and to reduce peak velocity in the central cavity region. In this paper, we examined its performance improvement using ATHOS code. The thermal-hydraulic condition in the region of secondary side of APR1400 steam generator is obtained using the ATHOS3 code. The effective mass for modal analysis is calculated using the void fraction, enthalpy, and operating pressure information from ATHOS3 code result. With the effective mass distribution along the tube, natural frequency and mode shape is obtained using ANSYS code. Finally, stability ratios and real mean squared displacements for selected tubes of the APR1400 steam generator are computed. From these results, the current design of the APR1400 steam generator are examined.

• Nuclear Engineering and Technology
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• v.51 no.3
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• pp.860-866
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• 2019

The lead element or its salts are good radiation shielding materials. However, their toxic effects are high. Due to less toxicity of bismuth salts, the radiation shielding properties of the bismuth salts have been investigated and compared to that of lead salts to establish them as a better alternative to radiation shielding material to the lead element or its salts. The transmission geometry was utilized to measure the mass attenuation coefficient (${\mu}/{\rho}$) of different salts containing lead and bismuth using a high-resolution HPGe detector and different energies (between 81 and 1333 keV) emitted from point sources of $^{133}Ba$, $^{57}Co$, $^{22}Na$, $^{54}Mn$, $^{137}Cs$, and $^{60}Co$. The experimental ${\mu}/{\rho}$ results are compared with the theoretical values obtained through WinXCOM program. The theoretical calculations are in good agreement with their experimental ones. The radiation protection efficiencies, mean free paths, effective atomic numbers and electron densities for the present compounds were determined. The bismuth fluoride ($BiF_3$) is found to have maximum radiation protection efficiency among the selected salts. The results showed that present salts are more effective for reducing the intensity of gamma photons at low energy region.

• Korean Journal of Materials Research
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• v.21 no.11
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• pp.596-603
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• 2011

The very high temperature gas reactor (VHTR) is one of the next generation nuclear reactors for its safety, long-term stability, and proliferation-resistance. The high operating temperature of over 800$^{\circ}C$ enables various applications with high energy efficiency. Heat is transferred from the primary helium loop to the secondary helium loop through the intermediate heat exchanger (IHX). The IHX material requires creep resistance, oxidation resistance, and corrosion resistance in a helium environment at high operating temperatures. A Ni-based superalloy such as Alloy 617 is considered as a primary candidate material for the intermediate heat exchanger. In this study, the microstructures of Alloy 617 crept in pure helium and air environments at 950$^{\circ}C$ were observed. The rupture time in helium was shorter than that in air under small applied stresses. As the exposure time increased, the thickness of outer oxide layer of the specimens clearly increased but delaminated after a long creep time. The depth of the carbide-depleted zone was rather high in the specimens under high applied stress. The reason was elucidated by the comparison between the ruptured region and grip region of the samples. It is considered that decarburization caused by minor gas impurities in a helium environment caused the reduction in creep rupture time.

• Nuclear Engineering and Technology
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• v.53 no.7
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• pp.2357-2363
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• 2021

Silicon carbide is widely used in radiation environments due to its excellent properties. However, when exposed to the strong radiation environment constantly, plenty of defects are generated, thus causing the material performance downgrades or failures. In this paper, the two-temperature model (2T-MD) is used to explore the defect recovery process by applying the electronic energy loss (Se) on the pre-damaged system. The effects of defect concentration and the applied electronic energy loss on the defect recovery process are investigated, respectively. The results demonstrate that almost no defect recovery takes place until the defect density in the damage region or the local defect density is large enough, and the probability of defect recovery increases with the defect concentration. Additionally, the results indicate that the defect recovery induced by swift heavy ions is mainly connected with the homogeneous recombination of the carbon defects, while the probability of heterogeneous recombination is mainly dependent on the silicon defects.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.102-109
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• 2018

Thistle is a perennial plant that is widely used for medicinal purposes. Information on the genetic diversity of thistle populations are great important for their conservation and germ plasmic utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish them from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the nuclear ribosomal DNA internal transcribed spacer (ITS) regions of genomic sequences to identify distinct Korean-specific thistle species via an amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the ITS intergenic region. We also developed a quantitative PCR assay using species-specific ITS primers, which allowed us to estimate the ratio of Korean-specific thistle species using varying ratios of mixed genomic DNA templates from the two species. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different countries.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.54-58
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• 2006

The genetic analyses of Acanthopanax senticosus Harms from Korea, China and Russia, were made by comparing the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA. The ITS region of A. senticosus was amplified by polymerase chain reaction (PCR) using the universal primers and then directly sequenced. The length of the ITS region including 162 bp 5.85 rRNA gene ranged from 608 bp (for Korean and Chinese) to 611 bp (for Russian). The G+C content of ITS region were 60.20% for Korean and Chinese plants and 60.06% for Russian plants. Sequence comparisons indicated that ITS regions of A. senticosus from Korea and China were identical, whereas the ITS sequence of A. senticosus from Russia showed 99.2% homology with the plants from Korea. Variation in sequences were attributable to 5 bp substitution such as transversion or insertion events. These results suggested that A. senticosus Harms from Korea and China were closely related in phylogenetic relationship compared to Russian. In addition, A. senticosus Harms were more similar to Kalopanax pictus than A. sessiliflorus in their ITS sequences.

• Nuclear Engineering and Technology
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• v.30 no.3
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• pp.222-234
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• 1998

The Westinghouse OT$\Delta$T DNB protection logic heavily restricts the operation region by applying the same logic for a full range of operating pressure in order to maintain its simplicity. In this work, a fuzzy neural network method is used to estimate the DNB protection limit using the measured average temperature and pressure of a reactor core. Fuzzy system parameters are optimized by a hybrid learning method. This algorithm uses a gradient descent algorithm to optimize the antecedent parameters and a least-squares algorithm to solve the consequent parameters. The proposed method is applied to Yonggwang 3&4 nuclear power plants and the proposed method has 5.99 percent larger thermal margin than the conventional OT$\Delta$T trip logic. This simple algorithm provides a good information for the nuclear power plant operation and diagnosis by estimating the DNB protection limit each time step.

• Nuclear Engineering and Technology
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• v.42 no.1
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• pp.97-108
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• 2010

Fluid-elastic instability and turbulence-induced vibration of steam generator U-tubes of a nuclear power plant are studied numerically to investigate the effect of design changes of support structures in the upper region of the tubes. Two steam generator models, Model A and Model B, are considered in this study. The main design features of both models are identical except for the conditions of vertical and horizontal support bars. The location and number of vertical and horizontal support bars at the middle of the U-bend region in Model A differs from that of Model B. The stability ratio and the amplitude of turbulence-induced vibration are calculated by a computer program based on the ASME code. The mode shape with a large modal displacement at the upper region of the U-tube is the key parameter related to the fretting wear between the tube and its support structures, such as vertical, horizontal, and diagonal support bars. Therefore, the location and the number of vertical and horizontal support bars have a great influence on the fretting wear mechanism. The variation in the stability ratios for each vibrational mode is compared with respect to Model A and Model B. Even though both models satisfy the design criteria, Model A shows substantial improvements over Model B, particularly in terms of having greater amplitude margins in the turbulence-excited vibration (especially at the inner region of the tube bundle) and better stability ratios for the fluid-elastic instability.