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Comparative Analysis of Acanthopanax senticosus Harms from Korea, China and Russia Based on the ITS Sequences of Nuclear Ribosomal DNA  

Han Hyo-Shim (Department of Biology, Sunchon National University)
Kim Doo-Young (Department of Biology, Sunchon National University)
Lee Kab-Yeon (Forest Seed Research Center, Korea Forest Research Institute)
Park Wan-Geun (Department of Forestry, Kangwon National University)
Cho In-Kyung (Department of Food Science and Technology, Nambu University)
Jung Jae-Sung (Department of Biology, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.19, no.1, 2006 , pp. 54-58 More about this Journal
Abstract
The genetic analyses of Acanthopanax senticosus Harms from Korea, China and Russia, were made by comparing the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA. The ITS region of A. senticosus was amplified by polymerase chain reaction (PCR) using the universal primers and then directly sequenced. The length of the ITS region including 162 bp 5.85 rRNA gene ranged from 608 bp (for Korean and Chinese) to 611 bp (for Russian). The G+C content of ITS region were 60.20% for Korean and Chinese plants and 60.06% for Russian plants. Sequence comparisons indicated that ITS regions of A. senticosus from Korea and China were identical, whereas the ITS sequence of A. senticosus from Russia showed 99.2% homology with the plants from Korea. Variation in sequences were attributable to 5 bp substitution such as transversion or insertion events. These results suggested that A. senticosus Harms from Korea and China were closely related in phylogenetic relationship compared to Russian. In addition, A. senticosus Harms were more similar to Kalopanax pictus than A. sessiliflorus in their ITS sequences.
Keywords
Acanthopanax senticosus Harms; ITS sequence; Siberian ginseng;
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