• 대한본초학회지
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• 제27권1호
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• pp.11-16
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• 2012

Objectives : In the theory of Korean medicine, Pear has long been considered to protect throat, bronchus and lung. Pear has been believed to remove sputum in Korean people. This study was conducted to investigate the effect of pear ethanol extract (PEE) on asthma induced by ovalbumin in mice. Methods : We investigated the effects of PEE on airway hyperresponsiveness to methacholine, production levels of ovalbumin (OVA) specific total immunoglobulin G (IgG), IgG1, IgG2a and IgE in serum and histopathological changes of lung tissues in asthamtic mice. Results : PEE decreased airway hyperresponsiveness to methacholine significantly compared to non-treated asthmatic mice (P<0.05). Level of OVA specific IgE in serum was lowered by oral administration of PEE effectively (P<0.05). In histopathological observation, administration of PEE reduced infiltration of immune cells into lung tissue. Conclusion : These results suggest that pear has anti-asthmaitc action and related mechanims are involved in anti-inflammatory action such as reducing level of OVA specific IgE and immune cell infiltration.

• 생명과학회지
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• 제16권7호
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• pp.1087-1089
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• 2006

Shrimp has efficient non-specific defense mechanisms based on activities of the hemocytes against pathogens. Up to now, it has been known that one of the non-specific immune reactions is related to mutual association among types of hemocyte, granular cell, semi-granular cell, and hyaline cell. In this study, we tried to know the effectiveness of feed-additives such as 5-aminolevulinic acid(ALA), chitosan, and hot-water extract of herb on immunity of shrimp (Penaeus chinensis) by hemocytic observation and SDS-PAGE analysis. Finally, we suggest a principle of the examination system for effects of various feed-additives.

• Animal cells and systems
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• 제11권1호
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• pp.33-38
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• 2007

Liposome-entrapped atypical Aeromonas hydrophila antigen was prepared to investigate the potential protective efficacy for A. hydrophila infection. Carp (Cyprinus carpio) were immunized orally with liposome-entrapped A. hydrophila antigen. After immunization, significantly more antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunized control group. The immunized carp were then challenged by immersion with $1{\times}10^{6}$ cfu/ml of A. hyrdophila for 60 min. Of the eight non-immunized carp, three carp died (62.5% survival), whereas five out of six (83.5%) of the immunized survived. Furthermore, development of skin ulcers was significantly inhibited in carp immunized with liposomes containing A. hydrophila antigen. These results suggest that liposomes containing A. hydrophila antigen have a potential for induction of protective immune responses against atypical A. hydrophila infection and also suggest the possibility of developing a vaccine that may ultimately be used for prevention of fish diseases.

• 약학회지
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• 제36권4호
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• pp.303-314
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• 1992

Experiments were performed on mice to investigate the effect of methionine diets (MET) on the immunotoxicity of ethanol. ICR female mice were divided into 5 groups, Met (Basal (B)+0.19% methionine(M), B+1.71% M and B+5.13%W) and ethanol(4%) were administered ad libitum for 21 days. The mice were evaluated for changes in immune status as measured by antibody titer, Arthus reaction, delayed type hypersensitivity (DTH), rosette forming cell(RFC) and plaque forming cell (PFC) to sheep red blood cells (S-RBC). To investigate the change of the non-specific immune response, the number of leukocytes in peripheral blood and phagocyte activity were measured. The results were summarized as follows: (1) The weight ratios of spleen and thymus to body weight were significantly increased by the B+0.19% M, B+0.57% M and B+1.71% M groups in comparison with control group(B), but B+5.13% M group was significantly decreased. (2) Humoral immune responses were significantly increased by the B+0.19% M and B+0.57% M groups in comparison with control group, but B+5.13% M group was significantly decreased. (3) Cellular immune responses were significantly decreased by the B+1.71% M and B+5.13% M groups in comparison with control group. (4) Phagocyte activities were significantly increased by the B+0.19% M, B+0.57% M and B+1.71% M groups in comparison with control groups, but B+5.13% M group was significantly decreased. (5) The number of circulating leukocyte was significantly increased in the B+0.19% M and B+0.57% M groups in comparison with control group, but B+5.13% M group was significantly decreased.

• 한국축산식품학회지
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• 제34권3호
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• pp.339-345
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• 2014

This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.

• Journal of Animal Science and Technology
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• 제52권4호
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• pp.337-346
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• 2010

우리 연구팀은 넙치를 대상으로 사료 내 발효마늘분말(fermented garlic powder, FGP)의 첨가에 의한 비특이적 면역반응과 어병세균(V. anguillarum, S. iniae, E. tarda)에 대한 질병 저항성에 대해 처음으로 보고한다. 동일한 단백질 함량(45%)과 칼로리(17.1 MJ/kg)를 갖은 4개의 실험사료는 발효마늘분말이 0%, 0.5%, 1% 및 2% (G-0, G-0.5, G-1 및 G-2)가 포함되도록 조성되었다. 실험은 3반복구로 수조 당 30마리의 어류를 대상으로 유수식 시스템에서 수행되었다. 5주간의 성장실험 종료 후, 각 수조에서 건강한 어류를 선별하여 어류의 항병력을 평가하기 위해 3가지 박테리아 부유액(1 ml)을 복강에 주사하였다. 5주간의 성장실험기간 동안에 성장률, 일간성장률, 사료전환효율, 단백질전환효율 및 생존율에서는 실험사료에 의한 유의적인 차이가 없었다. 그러나 사료섭취율은 발효마늘분말이 2% 첨가된 그룹에서 대조구와 비교하여 유의적으로 낮았다. 헤모글로빈, 헤마토크릿, 대식세포 활성 및 라이소자임 활성에서는 사료 내 발효마늘분말 함량이 증가함에 따라 유의적으로 증가하였다. 발효마늘분말 0.5% 첨가구의 혈장 중성지질은 대조구보다 유의적으로 낮았다. 누적사망률은 E. tarda의 경우를 제외한 모든 공격실험에서 발효마늘분말이 포함된 사료를 섭취한 어류가 대조구와 비교하여 유의적으로 낮았다. 이번 연구의 결과를 볼 때, 사료 내 발효마늘분말의 첨가는 넙치에 있어서 V. anguillarum과 S. iniae에 대한 항병력 및 비특이적 면역반응을 향상시킨 것으로 사료된다.

• 생명과학회지
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• 제30권9호
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• pp.826-833
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• 2020

Phospholipase C gamma (PLCγ)는 phosphatidylinositol을 가수분해하여 신호전달 과정에 참여하는 PLC의 주요한 isotype으로 γ-specific array의 특징적인 구조를 바탕으로 receptor tyrosine kinases 및 non-receptor tyrosine kinase 신호를 주로 매개한다. PLCγ1과 PLCγ2의 두 isozyme이 존재하며 다양한 세포에서 발현하여 cell proliferation, migration 및 differentiation 등 여러 세포작용을 조절하고 있다. 최근의 연구들에서 PLCγ 돌연변이가 cancer와 immune disease 및 brain disorder 등에 연관된다는 것이 밝혀지고 있으며 genetic model을 통해 PLCγ의 생리적·병리적 기능이 제시되었다. 본 리뷰에서는 최신의 연구 결과들을 바탕으로 PLCγ의 구조와 활성 조절 기전에 대해 기술하고 나아가 여러 질병의 발병과 진행에서 보고된 PLCγ의 돌연변이와 knockout 마우스를 활용한 연구 결과를 바탕으로 생리적·병리적 관점에서 PLCγ의 역할에 대해 고찰하였다.

• 한국어병학회지
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• 제15권1호
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• pp.25-35
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• 2002

Effects of medicinal herb extract on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Wormwood, Artemisia asiatica NAKAI and barrenwort, Epimedium koreanum NAKAI were mixed at a ratio of 7 : 3 (w/w) for 2-herbs extract and wormwood, barrenwort, Korean forsythia, Forsythia koreana NAKAI, chrysanthemum, Chrysanthemum zawadskii var. latilobum KITAMURA, peppermint, Mentha arvensis L. var, piperascens MALINV., great burnet, Snaguisorba afficinalis L., Lizard tail. Saururus chinensis BAILL., mulberry, Morus alba L., and star anise, Illicium varum HOOK, f, at the same weight for 9-herbs extract. Two-herbs of 9-herbs extract were prepared by heating after adding 10㎖ of distilled water per g of the herb mixtures. Fish (10.3$\pm$2.5g) were fed the experimental diets supplemented with the 2-herbs or 9-herbs extract at the different concentrations of 0%, 0.1%, 0.5% and 1% per kg diet for 12 weeks. Lysozyme and bactericidal activities of serum, and hematological characteristics were examined during experimental period. After feeding test period, all experimental groups were challenged with E. tarda. Lysozyme activity from the fish fed the diet supplemented with 0.1% or 0.5% of 2-herbs extract was significantly higher than the control. But there was no difference both in bactericidal activity and hematology among each group. Sixty seven % of relative percent survival values (RPS) in the group fed the diet supplemented with 0.1% of 2-herbs was higher than the other group and the control. These results suggest that supplenmentation of 0.1% of 2-herbs extract to a commercial diet may enhance disease resistance in olive flounder. Although both 0.1% and 0.5% 9-herbs extract did not improve non-specific immune reponses, they could enhance disease resistance of 53% RPS, respectively.

• BMB Reports
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• 제38권3호
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• pp.334-342
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• 2005

Recently, the existence of T cells with dual T cell receptor (TCR) in the immune system is generally accepted, while it has been controversial whether signals through one TCR would affect the functions of the other. In this study T cells expressing two different TCR were obtained from cross-hybrids of LCMV and AND TCR transgenic mice specific for the gp33 and peptide fragment of PCC (fPCC), respectively. Peptide stimulation demonstrated that the dual TCR T cells functioned independently in an antigen-specific manner. To examine whether the tolerance targeted for the one TCR affects the responsiveness of the other, the cross-hybrids were treated with gp33. Although T cells from F1 mice were rendered anergenic to gp33, no functional changes to fPCC were observed in terms of cellular proliferation and IL-2 secretion, suggesting that the dual TCR T cells remained reactive to fPCC. We therefore propose that signaling through the TCR is receptor-specific and 'negative dominance' of one TCR by tolerance induction is not applicable in this dual TCR system.

• 한국어병학회지
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• 제24권3호
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• pp.307-313
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• 2011

To examine the effects of Euglena rubra (E. rubra) feeding on Nile tiapia, Oreochromis niloticus were fed diets containing dried powder of E. rubra at 0.5 or 2% for 4 weeks. Growth, selected hematological and non-specific immune parameters were assessed at 2 and 4 weeks. There were no significant changes in body weigh gain and erythrocyte levels. However a significant and diet E. rubra level-related decrease in leukocyte level was noted. Significant increases were observed in respiratory burst activity (nitroblue tetrazolium reduction) and lysozyme activities following E. rubra feeding. These results indicate that E. rubra could be beneficial to fish, but excess feeding could toxic.