• Asian Journal of Innovation and Policy
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• 제10권1호
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• pp.108-131
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• 2021

The increase of international trade across countries and borders results in increased risks associated with the inflow of new pests and diseases. These risks are likely to be increased more rapidly due to climate change. Some countries implement strict regulations on imports to prevent these risks and protect biosecurity, food safety, and public health. However, the problems arise when the diseases and pests are found in a country where their economic structure largely depends on agricultural exports and cause ripple effects on other industries and ecosystems. Therefore, establishing an effective quarantine system is essential to protect and recover from the damage caused by non-native diseases and pests. This study's objectives are 1) analyzing the agricultural policies relate to the quarantine system on diseases and pests in Korea, 2) evaluating the Korea plant quarantine system's value, and 3) simulating plant quarantine policy strategies. We estimated the Korean quarantine system's benefits on diseases and pests to reach these objectives. The benefits are measured with a willingness to pay from respondents surveyed by the contingent valuation method (CVM). The CVM approach directly asks people how much they would willingly pay for food security. Finally, the Korean quarantine system's values are simulated with several policy scenarios and different scales of infection at the regional level. The results of this study can deliver policy implications on the quarantine system innovation in developing countries including Asia.

• 한국동물위생학회지
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• 제34권2호
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• pp.95-102
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• 2011

Foot-and-mouth disease (FMD) is one of the most infectious diseases affecting cloven-hoofed animals including cattle, sheep, goats, and pigs. Seven serotypes of foot-and-mouth disease virus with multiple subtypes within each serotype have been identified until now. In particular, it has been demonstrated that the outbreak of the serotype Asia1 reported from China, Mongolia and North Korea since 2005 is mostly classified into genetic group V. Though it has been recommended that Asia1 Shamir strain can be used as a high priority vaccine by World References Laboratory for FMD, the complete nucleotide sequences of the strain has not yet been determined. In this study, to be prepared for Asia1 type viruses that may be brought into Korea, the complete genome sequence of this vaccine strain Asia1 Shamir including its 5' and 3' non-coding region was identified.

• 대한수의학회지
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• 제63권4호
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• pp.35.1-35.10
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• 2023

Non-human primate (NHP) research faces challenges due to zoonosis risk and complex veterinary management yet lacks standardized guidelines for animal care. Therefore, we developed an advanced veterinary management protocol for NHP quarantine, anesthesia, and postoperative care. Three female 4 to 5-year-old cynomolgus monkeys were anesthetized and underwent various tests, including body weight, temperature, blood tests, urinalysis, microbiological monitoring, and physical and dental examinations. Ivermectin and medicated baths were administered to eradicate ectoparasites and endoparasites, and testing was repeated 30 days later. Following quarantine, we performed computed tomography and anesthesia maintenance for mastoidectomy. To relieve pain and maintain body weight, we administered tramadol intramuscularly 4 times/day for 3 days and meloxicam subcutaneously twice daily for 14 days. Feed replacements were provided. During the 33-day quarantine period, physical examinations revealed no abnormalities indicative of infectious diseases, and no specific clinical symptoms were observed. Through a preliminary test of anesthesia time, we selected ketamine 4 mg/kg + medetomidine 50 ㎍/kg for short experiments such as computed tomography, and ketamine 8 mg/kg + medetomidine 50 ㎍/kg for intubation. Ten days after mastoidectomy, NHPs consumed 100 kcal/kg and recovered their body weight. This study offers advanced veterinary management guideline for NHP research. Such protocols can lead to more standardized and ethical practices in NHP research, thereby enhancing the quality of studies on NHPs and the translation of findings to human health and disease.

• Journal of Veterinary Science
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• 제25권5호
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• pp.67.1-67.8
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• 2024

Importance: Carbapenem-resistant Enterobacteriaceae are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant Escherichia coli (CRE) in both humans and animals is important. Objective: We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats. Methods: E. coli strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE). Results: In total, 13 CRE isolates (0.13%) were identified from dogs possessing bla_NDM-5 along with β-lactamase genes, mostly blaCMY-2 (92.2%) and blaTEM-1 (53.8%). The commonly observed mutations were S83L and D87N in gyrA, S80I in parC, and S458A in parE. CRE carried non-beta-lactam resistance genes, with the majority being tet(B) (100%), sul (84.6%), and aac(3)-II (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected. Conclusions and Relevance: This investigation provides significant insight into the prevalence and molecular characteristics of bla_NDM-5-carrying E. coli in dogs. The co-existence of bla_NDM-5 and other antimicrobial resistance genes in E. coli potentially poses severe health hazards to humans.

• International journal of advanced smart convergence
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• 제9권3호
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• pp.169-175
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• 2020

A lot of research has been conducted on a system that collects and observes patients' health information in real time using Internet of Things (IoT) technology, and cares for and supports patients based on this. However, most studies have focused on underlying diseases such as diabetes or cardiovascular disease, and research on IoT systems to cope with respiratory infectious diseases such as COVID-19 is still insufficient. In a COVID-19 situation, the purpose of using an IoT respirator may vary depending on the user. In this paper, we design a system that can adequately cope with respiratory infectious diseases such as COVID-19 by applying IoT technology to respiratory protection. We categorize IoT respirator wearers into patients, medical staff, and self-quarantine persons, and define the purpose and use case of the IoT respirator system according to each classification. The proposed IoT respirator system was designed to achieve each purpose. We developed a prototype system consisting of a smart sensor, a communication module, and a non-motorized hooded respirator to show that the proposed IoT respirator system works.

• Fisheries and Aquatic Sciences
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• 제22권2호
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• pp.5.1-5.9
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• 2019

Background: Live fish import may lead to the unintended introduction of pathogens. We examined the monthly distribution of microbial pathogens in ornamental finfish imported into South Korea over a 6-month period. Results: Vibrio alginolyticus was detected in one lemon damsel in June and July; V. vulnificus was detected in one lemon damsel, one caerulean damsel, and one pearl-spot chromis and one ocellaris clownfish in July, April, and May, respectively; Photobacterium damselae was detected in one ocellaris clownfish and one caerulean damsel in June and July, respectively; V. anguillarum was detected in one pearl-spot chromis in February; V. harveyi was detected in one ocellaris clownfish and two mandarin fish in February and April, respectively; Yersinia ruckeri was detected in a pearlscale goldfish group in June and July and in two colored carp groups in July; and Lactococcus garvieae was detected in a lemon damsel group and a sutchi catfish group in July and May, respectively. European catfish virus, the only viral pathogen detected, was found in two sutchi catfish groups in May. Conclusion: This study is the first to identify pathogenic species and the presence or absence of pathogens (non-quarantine diseases) in imported ornamental finfish. These results demonstrate that various pathogens with the potential to harm indigenous fish populations can accompany ornamental finfish imported into South Korea.

• 한국임상수의학회지
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• 제29권1호
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• pp.63-67
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• 2012

Mosquitoes are the vectors of a number of viral diseases in cattle, such as Akabane disease, bovine ephemeral fever, Ainovirus infection, Chuzan virus infection, and Ibaraki disease. These diseases are transmitted from an infected animal to a non-infected host via the blood feeding of the vector. In Korea, the National Veterinary Research and Quarantine Services, Ministry for Food, Agriculture, Forestry and Fisheries is responsible for planning, implementation, laboratory investigations and reporting the results of the national surveillance program for mosquito-borne bovine diseases (MBD). The surveillance program, which was started in 1993, focused to determine the seroprevalence of each disease in cattle herds in space and time. From the epidemiological point of view, more important component of the surveillance program is to monitor infection rates in vectors for specific pathogens because this information is essential for a more precise understanding the dynamics of these diseases in a given environment and for determining risk of transmission. The aim of this study was to describe and compare methods for estimation of vector infection rates using maximum likelihood (MLE) and minimum infection rate in pooled samples. Factors affecting MLE such as number of pools, pooling size and diagnostic test performance are also discussed, assuming some hypothetical sampling scenarios for MBD.

• 대한수의학회지
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• 제53권1호
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• pp.25-28
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• 2013

The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle ($C_T$) of Salmonella spp. was $11.83{\pm}0.78$ while non-Salmonella spp. was $30.86{\pm}1.19$. Correlation coefficients of standard curves constructed using $C_T$ versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity ($R^2=0.993$; slope = 3.563). Minimum level of detection with the method was > $10^2$ colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.

• 한국산학기술학회논문지
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• 제19권12호
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• pp.353-362
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• 2018

주기적으로 발생하는 가축전염병으로 인한 국가적 손실이 매우 크다. 또한 우리나라에서 차량이동이 가축전염병의 주요 원인인 것으로 알려져 있다. 이 같은 상황에서, 본 연구는 축산차량의 이동 네트워크에서 축산시설의 구조적 위치와 전염병의 발생 사이의 관계를 실증분석하였다. 이를 위하여 KAHIS에서 제공하는 축산차량의 시설 진입 데이터를 활용하여 축산차량 이동 네트워크를 구축하였다. 구축된 네트워크에서 시설별로 중심성 지표를 도출하고, 전염병이 발생한 시설과 비발생 시설의 지표 평균을 비교하였다. 분석결과 첫번째 가설인 "전염병 발생시설의 연결정도 중심성은 비발생 시설보다 크다."는 옳은 것으로 나타났다. 전체시점과 시점별 분석을 실시한 결과 대부분의 자료에서 가축전염병이 발생한 시설의 연결정도 중심성이 비발생 시설에 비하여 통계적으로 크게 나타났다. 두 번째 가설인 "HPAI에서 발생시설과 비발생시설 사이의 연결중심성 차이가 FMD에서의 차이보다 작다."는 전체시점 자료에서 옳은 것으로 나타났다. 반면에 시점을 나누어서 분석한 결과에서는 뚜렷한 차이를 찾을 수 없었다. 연구의 결과에 따른 정책적 시사점은 다음과 같다. 첫 번째, 연결정도 중심성에 기반한 시설의 사전 관리가 필요하다. 두 번째, 우제류 농가의 경우 연결중심성에 기반한 관리정책의 도입이 보다 시급하다.

• The Plant Pathology Journal
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• 제20권3호
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• pp.165-171
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• 2004

A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7％ and 95.1％, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.