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http://dx.doi.org/10.14405/kjvr.2013.53.1.025

Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp.  

Shin, Seung Won (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Cha, Seung Bin (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Lee, Won-Jung (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Shin, Min-Kyoung (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Jung, Myunghwan (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Yoo, Anna (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Jung, Byeng Yeal (Bacterial Disease Division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Yoo, Han Sang (Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.53, no.1, 2013 , pp. 25-28 More about this Journal
Abstract
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle ($C_T$) of Salmonella spp. was $11.83{\pm}0.78$ while non-Salmonella spp. was $30.86{\pm}1.19$. Correlation coefficients of standard curves constructed using $C_T$ versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity ($R^2=0.993$; slope = 3.563). Minimum level of detection with the method was > $10^2$ colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
Keywords
invA; Salmonella spp; specific detection; SYBR Green real-time PCR;
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