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http://dx.doi.org/10.5423/PPJ.2004.20.3.165

Potential Biotypes in Korean Isolates of Bipolaris cactivora Associated with Stem Rot of Cactus  

Kim, Jeong-Ho (School of Agricultural Biotechnology and Center for Plant Molecular Genetics & Breeding Research, Seoul National University)
Jeoung, Myoung-Il (National Horticultural Research Institute, Rural Development Administration)
Hyun, Ick-Hwa (National Plant Quarantine Office)
Kim, Young-Ho (School of Agricultural Biotechnology and Center for Plant Molecular Genetics & Breeding Research, Seoul National University)
Publication Information
The Plant Pathology Journal / v.20, no.3, 2004 , pp. 165-171 More about this Journal
Abstract
A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7％ and 95.1％, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.
Keywords
Bipolaris cactivora; cactus stem diseases; colony morphology; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD); conidial type;
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  • Reference
1 Alcorn, J. L. 1983. Generic concepts in Drechslera. Bipolaris and Exserohilum. Mycotaxon 17: 1-86
2 Hawksworth, D. L., Kirk, O. M., Sutton, B. C. and Pegler, D. N. 1995. Anisworth & Bisbys Dictionary of the Fungi. $8^{th}$ ed. CAB International, Surrey. 616 pp
3 Manulis, S., Kogan, N., Reuven, M. and Ben-Yephet, Y. 1994. Use of the RAPD technique for identification of Fusarium oxysporum f. sp. dianthi from carnation. Phytopathology 84:98-101   DOI   ScienceOn
4 Rohlf, F. J. 1993. NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System. State Univ. of New York, Stony Brook
5 Sivanesan, A. 1987. Graninicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. CAB International Mycol. Papers 158: 1-261
6 Kim, W. G., Cho, W. D. and Jee, H. 2000. Occurrence of anthracnose on Indian fig cactus caused by Glomerella cingulata and Colletotrichum gloeosporioides. Plant Pathol. J. 16:294-296
7 GraJai-Martin, M. J., Simon, C. J. and Muehlbauer, F. J. 1993. Use of random amplified polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporum f. sp. pisi. Phytopathology 83:612-614   DOI   ScienceOn
8 Alcorn, J. L. 1988. The taxonomy of 'Helminthosporium' species. Ann. Rev. Phytopathol. 26:37-56   DOI   ScienceOn
9 Chase, A. R. 1982. Stem rot and shattering of Easter cactus caused by Drechslera cactivora. Plant Dis. 66:602-603   DOI
10 Huff, D. R., Bunting, T. E. and Plumley, K. A. 1994. Use of random amplified polymorphic DNA markers for the detection of genetic variation on Magnaporthe poae. Phytopathology 84:1312-1316   DOI   ScienceOn
11 Durbin, D. R., Davis, H. L. and Baker, F. K. 1955. A Helminthos-porium stem rot of cacti. Phytopathoogy 45:509-512
12 Hyun, I. H., Lee, S. D., Lee, Y. H. and Heo, N. Y. 1998. Mycological characteristics and pathogenicity of Fusarium oxysporum Schlecht. Emend. Snyd. & Hans. causing stem rot of cactus. Korean J. Plant Pathol. 14:463-466
13 Nicholson, P., Rezanoor, H. N. and Su, H. 1993. Use of random amplified polymorphic DNA (RAPD) analysis and genetic fingerprinting to differentiate isolates of race O, C and T of Bipolaris maydis. J. Phytopathol. 139:261-267   DOI   ScienceOn
14 Crowhurt, R. N., Hawthorne, B. T., Rikkerink, E. H. A. and Templeton, M. D. 1991. Differentiation of Fusarium solani f. sp. cucurbitate races 1 and 2 by random amplification of polymorphic DNA. Curro Gent. 20:391-396   DOI   ScienceOn
15 Hyun, J. W. and Park, W. M. 1996. Differentiation of Fusarium oxysporum f. sp.jragariae isolates by random amplified polymorphic DNA (RAPD) analysis. Korean J. Plant Pathol. 12:41-46
16 Kim, Y. H., Jun, O. K., Sung, M. J., Shin, J. S., Kim, J. H. and Jeong, M. 2000. Occurrence of Colletotrichum stem rot caused by Glomerella cingulata on Graft-cactus in Korea. Plant Pathol. J. 16:242-245
17 Guthrie, P. A. I., Magill, C. W, Frederiksen, R. A. and Odvody, G. N. 1992. Random amplified polymorphic DNA markers: A system for identifying and differentiating isolates of Colletotrichum graminicola. Phytopathology 82:832-835   DOI
18 Chang, M., Hyun, I. H. and Lee, Y. H. 1998. Bipolaris stem rot of cactus by Bipolaris cactivora (Petrak) Alcorn. Korean J. Plant Pathol. 14:661-663
19 Assigbetse, K B., Fernadez, M. P. Dubois and Geiger, J.P. 1994. Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random amplified polymorphic DNA (RAPD) analysis. Phytopathology 84:622-626   DOI   ScienceOn
20 Cassini, R. and Smith, I. M. 1988. Cochliobolus carbonum. In: European Handbook of Plant Diseases, ed. by l.M. Smith, J. Dunez, D.H. Phillips, and S.A. Archer. Blackwell Scientific Publications, Oxford, UK. 382 pp