Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Veterinary management protocol for non-human primates: quarantine, anesthesia, and postoperative care for mastoidectomy at animal research institutions

  • Yoon Beom Lee (Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation) ;
  • Woori Jo (Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation) ;
  • Eui-Suk Jeong (Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation) ;
  • Tae Ku Kang (Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation) ;
  • Gwang-Hoon Lee (Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation)
  • Received : 2023.08.10
  • Accepted : 2023.09.18
  • Published : 2023.12.31
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Abstract

Non-human primate (NHP) research faces challenges due to zoonosis risk and complex veterinary management yet lacks standardized guidelines for animal care. Therefore, we developed an advanced veterinary management protocol for NHP quarantine, anesthesia, and postoperative care. Three female 4 to 5-year-old cynomolgus monkeys were anesthetized and underwent various tests, including body weight, temperature, blood tests, urinalysis, microbiological monitoring, and physical and dental examinations. Ivermectin and medicated baths were administered to eradicate ectoparasites and endoparasites, and testing was repeated 30 days later. Following quarantine, we performed computed tomography and anesthesia maintenance for mastoidectomy. To relieve pain and maintain body weight, we administered tramadol intramuscularly 4 times/day for 3 days and meloxicam subcutaneously twice daily for 14 days. Feed replacements were provided. During the 33-day quarantine period, physical examinations revealed no abnormalities indicative of infectious diseases, and no specific clinical symptoms were observed. Through a preliminary test of anesthesia time, we selected ketamine 4 mg/kg + medetomidine 50 ㎍/kg for short experiments such as computed tomography, and ketamine 8 mg/kg + medetomidine 50 ㎍/kg for intubation. Ten days after mastoidectomy, NHPs consumed 100 kcal/kg and recovered their body weight. This study offers advanced veterinary management guideline for NHP research. Such protocols can lead to more standardized and ethical practices in NHP research, thereby enhancing the quality of studies on NHPs and the translation of findings to human health and disease.

Keywords

Acknowledgement

This research was funded by the Daegu-Gyeongbuk Medical Innovation Foundation in 2020 (project no. L22008 and R22048).

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