The present study was planned to examine the effect of different feeding regimens on milk urea concentration and milk protein concentration. The objectives are to describe the diurnal variations of milk urea (MU) concentration and to predict plasma urea (PU) concentration from MU concentration. Six lactating Murrah buffaloes were distributed in two groups and were fed two different diets in a crossover design. The diets consisted of leguminous crops as diet 1 (berseem (Trifolium alexandrinum)+concentrate mixture 1+wheat straw)) and non-leguminous crops as diet 2 (oats (Avana sativa)+concentrate mixture 2+wheat straw). All the diets were isocaloric and isonitrogenous. Each diet was fed to the animals for a period of 28 days, followed by a 10 day gap to obviate the carry over effect of the previous diet and then a switch over to the other diet. Digestibility trials were conducted on the last 7 days of each feeding period. Milk samples were collected on day 3, 7, 10, 14, 17, 21, 24 and 28 of the feeding period and blood samples were collected on the same day at morning within 30 minutes after morning milking. The average milk urea (MU) values (mg/dl) differed significantly (p<0.01) and were 44.83${\pm}$0.62 and 42.53${\pm}$0.73, respectively, for diets 1 and 2. Milk urea concentrations (mg/dl) also varied (p 0.01) among the days of feeding period, but were stabilized after 10th day of feeding period. In contrast, diets and days of feeding period had no significant effect on percent milk protein. Plasma urea concentration showed a significant (p<0.01) positive correlation (r = 0.93) with MU concentration. To predict the PU from MU the following equation was developed 'PU = 10.67${\pm}$0.76${\times}$MU (mg/dl) with $R^2$ = 0.87'. A clear diurnal variation of MU was found with lowered morning value (42.04${\pm}$0.68 mg/dl) than the evening value (45.32${\pm}$0.66 mg/dl). Present findings suggested that MU or PU concentration could be used as an indicator to monitor the feeding strategy. Plasma urea can be predicted from MU, whenever interpretation of milk urea data required consideration of diurnal variation.
The shelf life of 1,2-indandione/zinc (1,2-IND/Zn) solution and polyvinylpyrrolidone (PVP) solution, which are known as reagents for developing latent fingermarks deposited on the surface of thermal paper, was studied. The standard latent fingermarks used for comparisons were artificial latent fingermarks printed on thermally sensitive and non-sensitive surfaces with the same intensity. Upon treatment of standard latent fingermarks with the pre-mixed 1,2-IND/Zn and PVP solutions, the fingermarks could be successfully developed until 3 days after the preparation of the mixture. However, from the third day after mixing the reagents, blackening was observed on the surface of the thermal paper, indicating deterioration of the reagent performance. The 1,2-IND/Zn and PVP solutions separately stored without mixing in advance were mixed immediately before use, and the development efficiency of the latent fingermarks deposited on the surface of thermal paper was observed. The performance of the PVP solution decreased after 20 days from the preparation of the reagent. It was also found that the shelf life of 1,2-IND/Zn and PVP mixture was determined by the PVP solution. The effect of oxygen and moisture on the degradation of PVP was investigated. It was found that the performance of the PVP solution deteriorated because of the influence of moisture, though it was not affected by oxygen.
Rhodiola spp. and red ginseng have been used for food and medicinal applications in disease chemoprevention in many Asian countries. Increased oxidative stress by reactive oxygen species (ROS) has been proposed to be a major cause of muscle fatigue. The present study was designed to investigate the protective effects of a fermented hot-water extract mixture from Rhodiola sachalinensis and red ginseng (MFR) on cell damage and the antioxidant enzyme system in $H_2O_2$-induced oxidative stress in skeletal muscle cells. C2C12 myoblasts were treated with various concentrations of NFR (non-fermented Rhodiola sachalinensis extract), FR (fermented hot-water extract from Rhodiola sachalinensis) and MFR for up to 5 days after the standard induction of differentiation, followed by semi-quantitative RT-PCR. MFR treatment dose-dependently protected oxidative damage of C2C12 cells. The treatment with MFR also enhanced mRNA expressions of MyoD, Cu/Zn SOD, Mn-SOD and GPX up to 16%. These results indicate that MFR exerts an anti-oxidative effect through a mechanism (s) that may involve the up-regulation of antioxidant enzymes, which may be important for the cellular redox environment in muscle cells.
Seo, Yunhee;Park, Jiyeong;Cho, Myoung Rae;Chun, Jae Yong;Kim, Young Ho
Research in Plant Disease
/
v.20
no.3
/
pp.227-233
/
2014
The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 - 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 ($10^7$colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium.
Journal of the Korea institute for structural maintenance and inspection
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v.19
no.3
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pp.83-91
/
2015
Porous concrete consists of cement, water and coarse aggregate and has been used for the purpose of decreasing the earth environmental load such as air and water permeability, sound absorption, etc. However, the physical and mechanical properties of porous concrete changes due to compaction load during construction. For such a reason, the purpose of this study is to investigate the physical and mechanical properties of porous concrete according to the kinds of binder, the ratio of water to binder and target void ratio. In particular, this study has been carried out to investigate the influence of compaction load on the void ratio, strength and coefficient of permeability. Aggregate used in this study are by-products generated during production of crushed gravel with a maximum size of 13mm. The results of this study showed that the target void ratio, the coefficient of permeability and compressive strength of porous concrete had a close relationship with the void ratio, and it will be possible that the void ratio is suggested by the mix design of porous concrete. The compressive strength of porous concrete was the highest at the content of the expansive admixture of 5% and compared to non-mixture, 10% mixture of silica fume improved compressive strength about 32%. And in the result of the study to change the compaction load, the compressive strength increased from the load of 15kN, the void ratio decreased from the load of 0.8kN, the coefficient of permeability decreased from the load 35kN, respectively.
Oil pollution was world-wide prevalent treat to the environment, and the physic-chemical remediation technology of the TPH (total petroleum hydrocarbon) contaminated soil had the weakness that its rate was very slow and not economical. Bioremediation of the contaminated soil is a useful method if the concentrations are moderate and non-biological techniques are not economical. The aim of this research is to investigate the influence of additives on TPH degradation in a diesel contaminated soil environment. Six experimental conditions were conduced; (i) diesel contaminated soil, (ii) diesel contaminated soil treated with microbial additives, (iii) diesel contaminated soil treated with microbial additives and the mixture was titrated to the end point of pH 7 with NaOH, (iv) diesel contaminated soil treated with microbial additives and accelerating agents and (v) diesel contaminated soil treated with microbial additives and accelerating agents, and the mixture was titrated to the end point of pH 7 with NaOH. After 10 days, significant TPH degradation (67%) was observed in the DSP-1 soil sample. The removal of TPH in the soil sample where microbial additives were supplemented was 38% higher than the control soil sample during the first ten days. The microbial additives were effective in both the initial removal rate and relative removal efficiency of TPH compared with the control group. However, various environmental factors, such as pH and temperature, also affected the activities of microbes lived in the additives, so the pH calibration of the oil-contaminated soil would help the initial reduction efficiency in the early periods.
The properties of mung bean were investigated depending on hydrolysis condition. The results showed that enzyme treatments (${\alpha}$-amylase and protease each at 0.1% (w/w)) by varying hydrolysis temperature showed better properties than non-pretense treatment (control group). The treatment with 0.08% ${\alpha}$-amylase was best for optimum hydrolysis of mung bean starch The treatment using a mixture of 0.08% (w/w) ${\alpha}$-amylase and 0.12% (w/w) protease was best for optimum hydrolysis of meg bean protein. The effects of Hydrolysis time of mung bean showed that the optimum time was 60 and 90 min and there fore the optimum time was set at 60 min. These result showed that the best hydrolysis conditions of mung bean were the treatment at $60^{\circ}C$ for 60 min using a mixture of 0.08%(w/w) ${\alpha}$-amylase and 0.12% (w/w) pretense, with the sugar level shown at $5.8^{\circ}Brix$, reducing sugar at 2,022.13 mg% and crude protein at 7,666.17 mg%.
This study assessed the effects of treatment with sap extract from onion residues postharvest and purslane on the quality and quantity of organic onions. At the bending stage, onions treated with the sap extract showed vigorous growth, with higher plant heights, more leaves, and longer sheath lengths than untreated onions. The onion yield was significantly increased when the plant was treated with extracted sap as compared with that of untreated plants (p<0.05). The bulb weight distribution of onions in the mixed onion and purslane treatment was also significantly increased (~300 g) as compared with that of the other treatment (p<0.05). Except for CaO and S, the mineral content of the onions produced from plants treated with the onion and purslane extract mixture was higher than those of onions in the other treatment. The hardness of onions produced from plants treated with the onion and purslane extract was significantly increased (8% and 20%, respectively) as compared with that of onions produced from plants treated with the onion extract only or no treatment (p<0.05). However, there was no significant difference in the sugar contents of the onions produced from extract-treated and nonextract-treated plants. Postharvest, the content of inorganic components (phosphate, calcium, sulfuric acid, and manganese) was higher in soil treated with the onion extract than in soil treated with the onion and purslane extract and non-treated soil. It can be concluded that residues left after onion harvests and purslane extract can be used as natural and environmentally friendly materials for the cultivation of organic onions.
The purpose of this study is to utilize recycled concrete aggregates as permeable pavement materials. This study evaluates mechanical properties and durability of porous concrete depending on mixing rates of recycled aggregates and polyme. As a result, void ratio and permeability coefficient of porous concrete for pavement increased a little as mixing rate of recycled aggregates increased. Void ratio and permeability coefficient increased a lot as mixing rate of polymer increased. As polymer was mixed $20\%$, national regulation of permeable concrete for pavement($8\%$ and 0.01cm/sec) was met. Compressive strength and flexural strength decreased as mixing rate of recycled aggregates increased but they increased a lot as mixing rate of polymer increased. Even when recycled aggregates were mixed $75\%\;with\;10\%$ polymer mixed, national regulation of pavement concrete(18MPa and 4.5MPa) was met. In addition, regarding sliding resistance, BPN increased as mixing rate of recycled aggregates increased. But BPN decreased as polymer was mixed. Compared to crushed stone aggregates, abrasion resistance and freeze-thaw resistance decreased as mixing rate of recycled aggregates Increased. When polymer was mixed, abrasion resistance and freeze-thaw resistance improved remarkably. Compared to non-mixture, $10\%$ mixture of polymer improved abrasion resistance and freeze-thaw resistance about $8.6\%$ and 3.8times respectively.
Purpose: Currently, many chemicals are used in industrial and real life, and many substances are used in the form of a single substance, but most of them are used in the form of a mixture, and there is a need for a criterion for judging the danger of these substances. Method: Therefore, this study aims to confirm the risk criteria of the mixture through experimental studies on flammable mixtures in order to secure the effectiveness of the details of the existing Dangerous Goods Safety Management Act angerous Goods Judgment Criteria and to ensure the reliability and reproducibility of the dangerous goods judgment. Result: Experimental results show that alcohol flash point is mixed with water, which is a non-flammable liquid. Similar flash point trends occurred around 60% on an alcohol basis. In addition, in the case of flammable-combustible mixtures, there was little change in flash point if the flash point difference of the two materials was not large, and if the flash point difference of the two materials was low, the flash point tended to increase with the increase of the high flash point material. Conclusion: In the future, the test results may provide reference data on the experimental criteria for the flammable liquids that are cracked at the fire site.
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