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Bioremediation Efficiency of Oil-Contaminated Soil using Microbial Agents  

Hong, Sun-Hwa (Department of Environmental and Energy Engineering, Suwon University)
Lee, Sang-Min (Department of Environmental and Energy Engineering, Suwon University)
Lee, Eun-Young (Department of Environmental and Energy Engineering, Suwon University)
Publication Information
Microbiology and Biotechnology Letters / v.39, no.3, 2011 , pp. 301-307 More about this Journal
Abstract
Oil pollution was world-wide prevalent treat to the environment, and the physic-chemical remediation technology of the TPH (total petroleum hydrocarbon) contaminated soil had the weakness that its rate was very slow and not economical. Bioremediation of the contaminated soil is a useful method if the concentrations are moderate and non-biological techniques are not economical. The aim of this research is to investigate the influence of additives on TPH degradation in a diesel contaminated soil environment. Six experimental conditions were conduced; (i) diesel contaminated soil, (ii) diesel contaminated soil treated with microbial additives, (iii) diesel contaminated soil treated with microbial additives and the mixture was titrated to the end point of pH 7 with NaOH, (iv) diesel contaminated soil treated with microbial additives and accelerating agents and (v) diesel contaminated soil treated with microbial additives and accelerating agents, and the mixture was titrated to the end point of pH 7 with NaOH. After 10 days, significant TPH degradation (67%) was observed in the DSP-1 soil sample. The removal of TPH in the soil sample where microbial additives were supplemented was 38% higher than the control soil sample during the first ten days. The microbial additives were effective in both the initial removal rate and relative removal efficiency of TPH compared with the control group. However, various environmental factors, such as pH and temperature, also affected the activities of microbes lived in the additives, so the pH calibration of the oil-contaminated soil would help the initial reduction efficiency in the early periods.
Keywords
Bioremediation; denaturing gradient gel electophoresis (DGGE); microbial agents; oil-contaminated soil;
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1 Valeria, P. A., R. B. Vieira, F. P. Franca, and V. L. Cardoso. 2007. Biodegradation of effluent contaminated with diesel fuel and gasoline. J. Hazard. Mater. 140: 52-59.   DOI
2 Wei, Q. F., R. R. Mather, and A. F. Fotheringham. 2005. Oil removal from used sorbents using a biosurfactant. Bioresour. Technol. 96: 331-334.   DOI   ScienceOn
3 Yao, H., Z. He, M. J. Wilson, and C. D. Campell. 2000. Microbial biomass and community structure in a accumulation in soils increasing fertility and changing land use. Microb. Ecol. 40: 223-237.
4 Macek, T. M. and J. Kas, 2000. Exploitation of plants for the removal of organics in environmental remediation. Biotechnol. Adv. 18: 23-34.   DOI   ScienceOn
5 Margesin, R., D. Labbe, F. Schinner, C. W. Greer, and L. G. Whyte. 2003. Characterization of hydrocarbon-degrading microbial populations in contaminated and pristine Alpine soils. Appl. Environ. Microbiol. 6: 3085-3092.
6 Marques, A. V., S. C. Cunha des Santos, R. D. C. Casella, R. L. Vital, C. V. Sebastin, and L. Seldin. 2008. Bioremediation potential of a tropical soil contaminated with a mixture of crude oil and production water. J. Microbiol. Biotechnol. 18: 1966-1974.
7 Medina-Bellver, J. I., P. Marn, A. Delgado, A. Rodrguez- Sanches, E. Reyes, J. L. Ramos, and S. Marqus. 2005. Evidence for in situ crude oil biodegradation after the Prestige oil spill. Environ. Microbiol. 7: 773-779.   DOI   ScienceOn
8 Nam, B. H., B. J. Park, and H. S. Yun. 2008. Biodegradation of JP-8 by Rhodococcus fascians Isolated from Petroleum Contaminated Soil. Kor. J. Microbiol. Biotechnol. 23: 819- 823.
9 Mresi, W. and F. Schinner. 1991. An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazilium chloride. Biol. Fertil. Soils. 11: 210-220.   DOI   ScienceOn
10 Muhammad, A., J. Xu, Z. Li, H. Wang, and H. Yao. 2005. Effects of lead and cadmium nitrate on biomass and substrate utilization pattern of soil microbial communities. Chemosphere 60: 508-514.   DOI   ScienceOn
11 Philp, J. C., S. M. Bamforth, I. Singleton, and R. M. Atlas. 2005. Environmental pollution and restoration: A role for bioremediation, In R. M. Atlas, and J. Philp (eds.). Bioremediation. ASM Press, Washington, DC. U.S.A. 1-48.
12 Reichenauer, T. G. and J. J. Germida, 2008. Phytoremediation of organic contaminants in soil and groundwater. Green Sust. Chem. 1: 708-717.
13 An, Y. J., Y. H. Joo, I. Y. Hong, H. W. Ryu, and K. S. Cho. 2004. Microbial characterization of toluene-degrading denitrifying consortia obtained from terrestrial and marine ecosystems. Appl. Environ. Microbiol. 65: 611-619.
14 Betancur-Galvis, L. A., D. Alvarez-Bernal, A. C. Ramos- Valdivia, and L. Dendooven. 2006. Bioremediation of polycyclic aromatic hydrocarbon-contaminated saline-alkaline soils of the former Lake Texcoco. Chemosphere 62: 1749-1760.   DOI   ScienceOn
15 Huang, X. D., Y. El-Alawi, J. Gurska, B. R. Glick, and B.M. Greenberg. 2005. A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons (TPHs) from soils. Microchem. J. 81: 139-147.   DOI   ScienceOn
16 Garland, J. L. 1997. Analysis and interpretation of community level physiological profiles in microbial ecology. FEMS Microbiol. Ecol. 24: 289-300.   DOI   ScienceOn
17 Garland, J. L. and A. L. Mills. 1991. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community level sole carbon source utilization. Appl. Environ. Microbiol. 57: 2351-2359.
18 Glick, B. R. 2003. Phytoremediation: synergistic use of plants and bacteria to clean up the environment. Biotechnol. Adv. 21: 383-393.   DOI   ScienceOn
19 Ian, F. S. and J. F. Peter. 2003. A tribute to claude shannon (1916-2001) and a plea for more rigorous use of species richness, species diversity and the shannon-wiener index. Global Ecol. Biogeogr. 12: 177-179.   DOI   ScienceOn
20 Imsande, J. 1998. Iron, sulfur, and chlorophyll deficiencies: A need for an integrative approach in plant physiology. Physiol. Plant. 103: 139-144.   DOI   ScienceOn