• Tuberculosis and Respiratory Diseases
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• 제40권2호
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• pp.98-103
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• 1993

Background: Inactivation of retinoblastoma gene (Rb) has been observed in a variety of human cancers. Loss of heterozygosity (LOH) of Rb which is a common mode of allelic inactivation of Rb, has been known as a frequent genetic event in small cell lung cancer but it has been detected less frequently in non-small cell lung cancer. To define the role of Rb deletion in lung cancer, we investigated the genomic DNAs of 43 non-small cell lung cancers and 1 small cell lung cancer paired with normal lung tissues obtained by thoracotomy. Methods: The genomic DNAs were obtained by the digestion with proteinase K followed by phenol-chloroform extraction method. The genomic DNAs were digested by restriction endonuclease (EcoRI), separated by agarose gel electrophoresis, transferred to nylon membrane by Southern blot transfer and then hybridized with labelled Rb 1 probe which contains. 1.4 kb sized DNA sequence containing N-terminal portion of Rb. Results: In 26 squamous cell lung cancers, 16 cases were informative after EcoRI digestion and LOH of Rb was found in 10 cases (62.5%). In 17 adenocarcinomas of lung, 11 cases were informative and LOH of Rb was found in five cases (45.4%). The analysis of clinical parameters revealed no significant differences between the two groups with or without LOH of Rb in the aspects of age, sex, degree of differentiation, stage and smoking amount. Conclusions: These results suggest that Rb inactivation is also significantly involved in the molecular pathogenesis of non-small cell lung cancer.

• Journal of Applied Biological Chemistry
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• 제57권3호
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• pp.247-250
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• 2014

A lipid-soluble extract in ginseng marc was prepared by n-hexane extraction to evaluate its antioxidant and anticancer potential. A hexane extract of ginseng marc (HEGM) possessed a 2,2-diphenyl-1-picryl-hydrazyl free radical scavenging activity which was related to the amount of total phenolics. Also, HEGM showed a potent inhibitory activity on human non-small cell lung cancer (A549, $GI_{50}=34.0{\mu}g/mL$) and colon cancer (SNU-C4, $GI_{50}=45.2{\mu}g/mL$) cells proliferation in vitro in a concentration-dependent manner as did the hexane extract of ginseng with $GI_{50}$ values of $20.0{\mu}g/mL$ in A549 and $37.0{\mu}g/mL$ in SNU-C4. These results imply that HEGM can be utilized as an antioxidant and anticancer substance.

• Environmental Engineering Research
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• 제12권1호
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• pp.16-20
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• 2007

Toxicity of metal plating wastewater was evaluated by using acute toxicity tests on Daphnia magna. To identify toxicants of metal plating wastewater, several manipulations such as solid phase extraction (SPE), ion exchange and graduated pH adjustment were used. The SPE test had no significant effect on baseline toxicity, suggesting absence of toxic non-polar organics in metal plating wastewater. However, anion exchange largely decreased the baseline toxicity by 88%, indicating the causative toxicants were inorganic anions. Considering high concentration of chromium in metal plating wastewater, it is thought the anion is Cr(VI) species. Graduated pH test showing independence of the toxicity on pH change strongly supports this assumption. However, as revealed by toxicity confirmation experiment, the initial toxicity of metal plating wastewater (24-h TU=435) was not explained only by Cr(VI) (24-h TU = 725 at $280\;mg\;L^{-1}$). Addition of nickel($29.5\;mg\;L^{-1}$) and copper ($26.5\;mg\;L^{-1}$) largely decreased the chromium toxicity up to 417 TU, indicating antagonistic interaction between heavy metals. This heavy metal interaction was successfully predicted by an equation of 24-h $TU\;=\;3.67\;{\times}\;\ln([Cu]\;+\;[Ni])\;+\;79.44$ at a fixed concentration of chromium.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제15권7호
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• pp.4516-4521
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• 2014

Digital watermarking for digital image authentication and integrity schemes are based on fragile watermarking and can detect any modifications in a watermark embedded image by comparing the embedded watermark with the regenerated watermark. Therefore, the digital watermark for image authentication and integrity should be erased easily when the image is changed by digital image processing, such as scaling or filtering etc. This paper proposes an effective tamper detection scheme for digital images. In the proposed scheme, the original image was divided into many non-overlapping $2{\times}2$ blocks. The digital watermark was divided into two LSB of each block and the image distortion was imperceptible to the human eye. The watermark extraction process can be used to determine if the watermarked image has been tampered. The experimental results successfully revealed the effectiveness of the proposed scheme.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• 제9권2호
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• pp.148-154
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• 2016

In recent year, the interests of PQ is increase due to the increasing of non-linear load and distributed power sources in power system. For the parameters detection and feature extraction of PQ, and the PQ improvement method, continuous power signal monitering is needed. In this paper, the power signal compression and reconstruction method is suggested for power signal monitering. The power signal is compressed using DCT that has good compression performance, and the compressed signal is reconstructed through IDCT. And for the higher compression rate, DCT coefficients are arranged by magnitude in compression process, and in recouction process DCT coefficients are rearranged to original frequency position. The synthesized signal according to the IEC standard is used used in compression and reconstruction simulations. The performances of the proposed method are verified by comparing the error between synthesized signal and reconstructed signal.

• Economic and Environmental Geology
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• 제30권6호
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• pp.587-601
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• 1997

This study had three purposes: (1) to investigate the enrichment levels and dispersion patterns of potentially toxic elements in the rock-soil-plant system; (2) to evaluate the uptake ratios of heavy metals from soils into plants and (3) to assess the chemical speciation of heavy metals in soils. Rock, surface soil and plant samples were collected in the Boeun area underlain by black shales of the Okchon Zone. These samples were analyzed for multi-elements using INAA, ICP-AES and AAS. The maximum abundance of U in black shales is 16 mg/kg and radioactivity counts up to 300 cpm. In particular, Mo, V, Ba, Cd, Pb and U are enriched in black shales. Most of soils derived from black shales show high concentrations of U, As, Mo, Ba, Cu, Cd, Pb, Zn and mean concentrations of As and Mo in soils (20 mg/kg of As and 6.6 mg/kg of Mo) are higher than the permissible level suggested by Kloke (1979). Enrichment index values of soils are calculated and higher than 1.0 in the black shale area with the highest value of 6.4. Mean concentration of Cd in plants is higher than those of Cu, Pb and Zn. The concentration of Cd in plant species decreases in the order of Chinese cabbage > red pepper > soybean=sesame > rice stalk > com > rice grain. The biological absorption coefficients (BAC) in plants are in the order of Cd > Zn=Cu > Pb, which suggests that Cd is more bioavailable to plants than Cu, Pb and Zn. From the results of sequential extraction analysis of soils, relatively high proportion of Cu, Pb and Zn are present as residual fractions whereas that of Cd as non-residual fractions. Cadmuim occurs predominantly as exchangeable/water-acid soluble phase in soils, and Cd is more mobile and bioavailable than Cu, Pb and Zn.

• Journal of Digital Convergence
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• 제11권12호
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• pp.295-302
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• 2013

Many reported methods assume that the people in an image or an image sequence have been identified and localization. People detection is one of very important variable to affect for the system's performance as the basis technology about the detection of other objects and interacting with people and computers, motion recognition. In this paper, we present an efficient linear discriminant for multi-view people detection. Our approaches are based on linear discriminant. We define training data with fisher Linear discriminant to efficient learning method. People detection is considerably difficult because it will be influenced by poses of people and changes in illumination. This idea can solve the multi-view scale and people detection problem quickly and efficiently, which fits for detecting people automatically. In this paper, we extract people using fisher linear discriminant that is hierarchical models invariant pose and background. We estimation the pose in detected people. The purpose of this paper is to classify people and non-people using fisher linear discriminant.

• Research in Plant Disease
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• 제20권1호
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• pp.15-20
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• 2014

A rapid, user-friendly and simple immune-chromatographic dipstick kit named 'rapid immune-gold strip' (RIGS) kit was developed in a novel single strip format to detect on-site detection of Tomato spotted wilt virus (TSWV). Immunoglobulin G (IgG) from polyclonal antisera raised in rabbits against TSWV was purified through protein-A affinity chromatography and then the purified TSWV-IgG was conjugated to colloidal gold nano-particles which served as a test line on nitrocellulose membrane. Protein A that non-specifically binds to TSWV antibody was used as a control line on the same strip. The diagnosis process with the TSWV-RIGS involves simply grinding the suspect plant sample in a bag that contains the extraction buffer and inserting the strip the bag. Results can be seen in 2-5 minutes. The flow of the complexes of gold particles coated with TSWV-IgG and a crude sap from TSWV-infected pepper, tobacco and tomato plants resulted in intensive color formed on the test lines proportional to the concentrations of TSWV. The RIGS-TSWV kit did not show any cross-reactions against other tomato-infecting viruses unrelated to TSWV. These results indicate that the TSWV-RIGS kit is highly sensitive and is not required for laboratory training and experience prior to testing. The TSWV-RIGS kit is suitable for on-site detection of suspect TSWV-infected plants as well as for laboratory diagnosis.

• Journal of the Institute of Electronics Engineers of Korea SD
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• 제39권3호
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• pp.73-84
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• 2002

Fast and accurate new capacitance determination methodology for non-uniform complicated multi-layer VLSI interconnects is presented. Since a capacitance determination of intricate multi-layer interconnects using 3-dimensional field-solver is not practical, quasi-3-dimensional methodology is presented. Interconnects with discontinuity (i.e., bend structure and different spacing between lines, etc.) are partitioned. Then, each partial capacitance of divided parts is extracted by using 2-dimensional extraction methodology. For a multi-layer interconnects with shielding layer, the system can be simplified by investigating a distribution of charge in it. Thereby, quasi-3-dimensional capacitance for multi-layer interconnects can be determined by combining solid-ground based 2-dimensional capacitance and shielding effect which is independently determined with layout dimensions. This methodology for complicated multi-layer interconnects is more accurate and cost-efficient than conventional 3-dimensional methodology It is shown that the quasi-3-dimensional capacitance methodology has excellent agreement with 3-dimensional field- solver-based results within 5% error.

• Journal of the Korea Society of Computer and Information
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• 제19권10호
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• pp.43-53
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• 2014

Gunnery has been used to detect and classify artilleries. In this paper, we used electro-optical data to get the information of muzzle flash from the artilleries. Feature based approach was applied; we first defined features and sub-features. The number of sub-features was 38~40 generic sub-features, and 2 model-based sub-features. To classify multiclass data, we introduced tree structure with clustering the classes according to the similarity of them. SVM was used for each non-leaf nodes in the tree, as a sub-classifier. From the data, we extracted features and sub-features and classified them by the tree structure SVM classifier. The results showed that the performance of our classifier was good for our muzzle flash classification problem.