• Proceedings of the KSRS Conference
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• 2007.10a
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• pp.460-463
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• 2007

COMS (Communication Ocean Meteorological Satellite) is the geostationary satellite being developed by Korea Aerospace Research Institute for multi-mission: experimental communication, ocean monitoring and meteorological observations. The COMS operation is controlled by the on-board software running on the spacecraft central computer. The software is written in ADA language and developed under the software life cycle: Requirement analysis, Design, Implementation, Component test and Integration test. Most functional requirements are tested at component level on a software component testing tool, ATTOL. ATTOL provides a simple way to define the test cases and automates the test program generation, test execution and test analysis. When two or more verified components are put together, the integration test starts to check the non-functional requirements: real-time aspect, performance, the HW/SW compatibility and etc. This paper introduces the COMS on-board software and explains what to test and how to test the on-board software at component level using ATTOL.

• Convergence Security Journal
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• v.15 no.5
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• pp.29-35
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• 2015

The network security encryption type is divided into two, one is point-to-point, second method is link type. The level of security quality attributes are a system security quality requirements in a networked environment. Quality attributes can be observed and should be able to be measured. If the quality requirements can be presented as exact figures, quality requirements are defined specifically setting quality objectives. Functional requirements in the quality attribute is a requirement for a service function which can be obtained through the encryption. Non-functional requirements are requirements of the service quality that can be obtained through the encryption. Encryption quality evaluation system proposed in this study is to derive functional requirements and non-functional requirements 2 groups. Of the calculating measure of the evaluation index in the same category, the associated indication of the quality measure of each surface should be created. The quality matrix uses 2-factor analysis of the evaluation for the associated surface quality measurements. The quality requirements are calculated based on two different functional requirements and non-functional requirements. The results are calculated by analyzing the trend of the average value assessment. When used this way, it is possible to configure the network security encryption based on quality management.

• BMB Reports
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• v.44 no.4
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• pp.279-284
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• 2011

The glutathione S-transferase (GST) system is useful for increasing protein solubility and purifying soluble GST fusion proteins. However, purifying half of the GST fusion proteins is still difficult, because they are virtually insoluble under non-denaturing conditions. To optimize a simple and rapid purification condition for GST-pyruvate kinase muscle 2 (GST-PKM2) protein, we used 1% sarkosyl for lysis and a 1 : 200 ratio of sarkosyl to Triton X-100 (S-T) for purification. We purified the GST-PKM2 protein with a high yield, approximately 5 mg/L culture, which was 33 times higher than that prepared using a conventional method. Notably, the GST-high-temperature requirement A2 (GST-HtrA2) protein, used as a model protein for functional activity, fully maintained its proteolytic activity, even when purified under our S-T condition. This method may be useful to apply to other biologically important proteins that become highly insoluble in the prokaryotic expression system.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.04a
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• pp.586-589
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• 2012

Web Services have been utilized in a wide variety of applications and have turned into a key technology in developing business operations on the Web. Originally, Web Services can be exploited in an isolated form, however when no single Web Service can satisfy the functionality required by a user, there should be a possibility to compose existing services together in order to fulfill the user requirement. However, since the same service may be offered by different providers with different non-functional Quality of Service (QoS), the task of service selection for Web Service composition is becoming complicated. Also, as Web Services are inherently unreliable, how to deliver reliable Web Services composition over unreliable Web Services should be considered while composing Web Services. In this paper, we propose an approach on a QoS-aware Web Service selection for reliable Web Service composition. In our approach, we select and classify Web Services using Decision Tree based on QoS attributes provided by the client. Service classifier will improve selection of relevant Web Services early in the composition process and also provide flexibility to replace a failed Web Services with a redundant alternative Web Services, resulting in high availability and reliability of Web Service composition. We will provide an implementation of our proposed approach along with efficiency measurements through performance evaluation.

• Biomolecules & Therapeutics
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• v.31 no.1
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• pp.48-58
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• 2023

Interferon regulatory factor 3 (IRF3) integrates both immunological and non-immunological inputs to control cell survival and death. Small GTPases are versatile functional switches that lie on the very upstream in signal transduction pathways, of which duration of activation is very transient. The large number of homologous proteins and the requirement for site-directed mutagenesis have hindered attempts to investigate the link between small GTPases and IRF3. Here, we constructed a constitutively active mutant expression library for small GTPase expression using Gibson assembly cloning. Small-scale screening identified multiple GTPases capable of promoting IRF3 phosphorylation. Intriguingly, 27 of 152 GTPases, including ARF1, RHEB, RHEBL1, and RAN, were found to increase IRF3 phosphorylation. Unbiased screening enabled us to investigate the sequence-activity relationship between the GTPases and IRF3. We found that the regulation of IRF3 by small GTPases was dependent on TBK1. Our work reveals the significant contribution of GTPases in IRF3 signaling and the potential role of IRF3 in GTPase function, providing a novel therapeutic approach against diseases with GTPase overexpression or active mutations, such as cancer.

• The Korean Journal of Food & Health Convergence
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• v.9 no.6
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• pp.9-19
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• 2023

Lactobacillus plantarum and Leuconostoc mesenteroides are crucial functional starters and predominant isolates in a wide range of fermented foods, particularly kimchi, whose constituents exhibit bioactive properties. We previously developed a methodology using anion exchange resins to purify peptidyl compounds from Lb. plantarum LBP-K10. Antibacterial cultures of Lb. plantarum LBP-K10 were obtained from the respective cultures' supernatants and filtrates. However, conclusive evidence of the efficacy of kimchi filtrates in eradicating pathogenic bacteria is lacking. We aimed to simulate the potential effects of antibacterial filtrates that contained antibacterial compounds which were derived from cultures of Lb. plantarum LBP-K10. We acquired the kimchi filtrates using a combination of centrifugation and filtration methodologies, without the requirement for inoculation. The filtered liquid from Chinese cabbage kimchi, inoculated with Lb. plantarum LBP-K10 as a starter culture, and the non-inoculated liquid from Chinese cabbage kimchi (referred to as CCK and CCKRef, respectively) were were examined. CCK demonstrated greater inhibitory activity and a more significant bactericidal effect against the bacterial indicator strains. The minimum inhibitory concentration demonstrated comparable outcomes in tests against both Gram-positive and Gram-negative bacteria. This research offers a groundbreaking examination that displays the effectiveness of profiling peptidyl compounds within kimchi filtrates for curing bacterial infections.

• Journal of Korean Society of Archives and Records Management
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• v.7 no.2
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• pp.113-138
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• 2007

This study was started from a critical mind for the construction records management in South Korea and examined the state of the construction records management system of the Korea Rail Network Authority which is constructing the biggest Express Railway Construction of National Project in Korea. The Methodology of this study is based on the functional requirement of the Records Management International Standard, ISO 15489, which standardized the record management process in the cases of Record Capture, Declaration, Classification, Storage, Access, Tracking and Disposition. In regard to the functional requirements from Capture to Disposition, each level(title) consists of 3 to 4 items, for a total of 22 principles in all. The estimation was created for each of the 22 categories using 4 levels: observance, partial observance, insufficiency and non observance. And pointing out the problem, suggesting the improvements for the Korea Rail Network Authority and structural improvements according to the KS X ISO 15489 principles.

• Journal of KIISE:Software and Applications
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• v.29 no.4
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• pp.211-224
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• 2002

As system become larger and more complex, it is important to correctly analyze and specify user's requirements. Use case modeling is widely used in Object-Oriented Analysis and Design(OOAD) and Component-Based Development(CBD). It is useful to mitigate the complexity of the requirements analysis. However, use cases are difficult to be structured, to explicitly represent non-functional requirements, and to analyze what is affected by changes of use cases. To alleviate these problems, we propose scenario-based goal-oriented approach for use case modeling. The approach is to apply goal-oriented analysis method to use case model. Since goal-oriented analysis method is not systematic and heuristics is considerably involved, we adopted scenarios as the basis for the goal extraction. The proposed method is applied to City Bus Information Subsystem(CBIS) in Intelligent Transport Systems(ITS) domain. The proposed approach helps software engineer to analyze what is affected by use case's changes and represent non-functional requirements.

• Spatial Information Research
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• v.16 no.2
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• pp.157-171
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• 2008

With emerging technologies on wireless networks and mobile computing environment, a number of researches have been carried out for ubiquitous computing. An important functional requirement of ubiquitous computing is to handle location data with ease. With the increase of accidents in large complex buildings. move attention is being paid to indoor spaces and evacuation. However, most currently used evacuation-related applications are simulation based on hypothetical data. Also, since they use non-georeferenced CAD data, it is not easy to integrate them with indoor positioning devices. With the recent progress of indoor positioning systems, the simulators can be enhanced to real-time evacuation systems. As a preliminary stage to make such systems possible, this study proposes using a georeferenced data and evacuation simulation. This study used GIS data and Cellular Automata theory an the algorithm for the movement of the evacuee.

• Journal of the Korea Safety Management & Science
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• v.16 no.2
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• pp.43-52
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• 2014

In weapon systems development, live fire tests have been frequently adopted to evaluate the performance of the systems under development. Therefore, it is necessary to ensure safety in the test ranges where the live fire tests can cause serious hazards. During the tests, a special care must be taken to protect the test and evaluation (T&E) personnel and also test assets from potential danger and hazards. Thus, the development and management of the range safety process is quite important in the tests of guided missiles and artillery considering the explosive power of the destruction. Note also that with a newly evolving era of weapon systems such as laser, EMP and non-lethal weapons, the test procedure for such systems is very complex. Therefore, keeping the safety level in the test ranges is getting more difficult due to the increased unpredictability for unknown hazards. The objective of this paper is to study on how to enhance the safety in the test ranges. To do so, an approach is proposed based on model-based systems engineering (MBSE). Specifically, a functional architecture is derived utilizing the MBSE method for the design of the range safety process under the condition that the derived architecture must satisfy both the complex test situation and the safety requirements. The architecture developed in the paper has also been investigated by simulation using a computer-aided systems engineering tool. The systematic application of this study in weapon live tests is expected to reduce unexpected hazards and test design time. Our approach is intended to be a trial to get closer to the recent theme in T&E community, "Testing at the speed of stakeholder's need and rapid requirement for rapid acquisition."