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Development of SSR markers for genetic mapping of Korean ginseng and authentication of Korean ginseng cultivars

  • Kim, Nam-Hoon;Choi, Hong-Il;Jung, Ju-Yeon;Choi, Beom-Soon;Ahn, In-Ok;Lee, Joon-Soo;Yang, Tae-Jin
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2010.10a
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    • pp.11-11
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    • 2010
  • The Korean ginseng, Panax ginseng C. A. Meyer is a popular medicinal herb in Araliaceae. Genetic map in crops provides valuable information for breeding, genetic and genomic researches. However, little information is available for construction of genetic map in ginseng. Up to now, we have produced large amounts of expressed sequence tags (ESTs) from four ginseng cultivars (37Mb, 49Mb, 39Mb, 47Mb from Gopoong, Gumpoong, Chunpoong and Yunpoong respectively using pyrosequencing technique and 5Mb from normalized full-length cDNA library of Chunpoong) to obtain comprehensive information of gene expression, and constructed EST database including ESTs from public database. Till now, we designed 261 SSR primer sets using EST sequences and identified 106 intergenic polymorphic markers. And 44 of the 106 showed polymorphisms among panax ginseng cultivars. Among 44 markers, 27 SSR polymorphic markers were inspected to 51 $F_2$ population from Yunpoong x Chunpoong, which showed good at the fitness of Mendellian segregation ratio 1:2:1. To enrich the number of markers, and thus construct high resolution genetic map which can be used as frame map for further genome sequencing. we are planning to develop large scale EST-derived SNP markers which are available in the F2 population. This study provides genetic information as well as foundation for ginseng researches such as genetics, genomics, breeding, and the final goal for whole genome sequencing. This study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Grant No. 609001-051SB210).

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Integrated Korean Flora Database: A Versatile Web-based Database for Dissecting Flora Investigations

  • Yeon, Jihun;Kim, Yongsung;Kim, Hyejeong;Kim, Juhyun;Park, Jongsun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.16-16
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    • 2018
  • Flora investigations have been conducted by many researchers for a long time in Korea. Even though large amount of investigation data has been accumulated, there is no accurate statistics or database because most of data were published in a printed form. We developed a web-based database of flora investigation, named as the Integrated Korean Flora Database (http://www.floradb.net/) to understand distribution patterns and habitats of plants in Korea. Till now, 480 published paper, 356 thesis, 76 reports and books, and 8 unpublished papers written in between 1962 and 2017 were collected and their species lists from 280 papers were parsed into the database. From 124,105 records, 3,100 species belonging to 206 families and 965 genera were identified via comparing with two major Korean plant species lists. 55 endangered species, 159 endemic species, and 367 rare species were identified. The most frequently surveyed species were Commelina communis in herbaceous and Rosa multiflora in woody plants. Microclimate data provided by Korea Meteorological Administration were also integrated and analyzed to assign cold hardness zones for each species. By comparing minimum temperature (<2%) acquired from automated weather stations (AWS) near by plant species, 6a to 10b zones (7b is the most frequent zone) were identified. Integrated Korean Flora Database will be a fundamental platform of korea flora investigation as well as a new standard for classifying distribution of plants based on accurate microclimate data. Moreover, it can also provide evidences of investigated plant species, such as specimen and/or pictures with connecting to the InfoBoss Cyber Herbarium (http://herbarium.infoboss. co.kr/) and Biodiversity Observation Datbase (BODB; http://www.biodiversitydb.org/).

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Effects of Specific Exercises on Motor Function Recovery In Rats With Experimental Spinal Cord Injury (척수 손상 쥐에 실시한 특정 과제 운동이 운동 행동에 미치는 영향)

  • Jun, Kyoung-Hee
    • Physical Therapy Korea
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    • v.18 no.1
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    • pp.93-103
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    • 2011
  • This study was implemented to verify the feasibility of motor function recovery and the appropriate period for therapy. The research began with spinal laminectomy of 40 white rats of Sprague-Dawley breed and induced them spinal crush injury. Following results were obtained by using the modified Tarlov test (MTT), Basso, Beattle, Bresnahan locomotor rating scale (EBB scale) and modified inclined plate test (MIPT). First, the measurement using the MTT confirm that the most severe aggravation and degeneration of functions are observed two days after induced injury, and no sign of neuromotor function recovery. Second, better scores were achieved by open-ground movement group on BBB locomotor rating scale test, and weight-bearing on inclined plate group show better performance on MIPT. Third, both BBB and MIPT scale manifested the peak of motor function recovery during 16th day after the injury and turn into gradual recovery gradient during 16th to 24th. Fourth, the control group showed functional recovery, however, the level of recovery was less significant when compared with group open-ground movement group and weight-bearing on inclined plate group. Hence, it was clearly manifested that the lumbar region of the spinal cord had shown the best performance when its functions were measured after the execution of specific physical training; therefore it indicated the possibility of learning specific task even in damaged lumbar regions. Thus it is expected to come out with better and more effective functional recovery if concentrated physical therapy was applied starting 4 days after the injury till 16 days, which is the period of the most active recovery.

The SPM Study on the Change of Titanium Surface Roughness following Airpowder Abrasive and Application Time of Citric Acid (공기-분말 연마와 구연산의 적용시간에 따른 임프란트 표면 거칠기의 변화에 관한 주사탐침현미경적 연구)

  • Park, Min-Seo;Chung, Chin-Hyung;Lim, Sung-Bin
    • Journal of Periodontal and Implant Science
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    • v.30 no.4
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    • pp.821-836
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    • 2000
  • The Peri-implantitis causes inflammation of periodontal tissue and bone loss. It contaminates surface of implants. Therefore, guided bone regeneration has been used for the treatment of this disease. For the reosseointegration of the exposed surface, various mechanical and chemical methods have been used for cleaning and detoxication of implant surface. Among these methods, air-powder abrasive and oversaturated citrate are known to be most effective. However, these treatments may deform implant surface. In this research, changes of surface roughness they were examined. 10 experimental machined titanium cylinder models were fabricated to be used for control groups. Each of them was air powder abraded for 1 minute and they were named group 1. And then, group 1 were burnished with cotton pellets soaked with citrate for 30 seconds(Group 2), 1 minute(Group 3), 3 minutes(Group 4), and 5 minutes(Group 5) burnishing were applied for grouping respectively. Each group were examined with SPM, and their surface roughness were measured and analyzed. 1. Surface roughness of titanium decreased when it was air-powder abraded for 1 minute. It was statistically significant. 2. When Air-powder abraded titanium were treated with citrate for 3 minutes, Their surface roughness was the lowest. Titanium treated for 1 minute was the second lowest and 30 seconds was the third and titanium burnished for 5 minutes was the highest. 3. Surface roughness of titanium which was treated with citrate was decreased till 3 minutes, which was statistically significant. There was no statistical significance from 30 seconds to 1 minute and from 1 minute to 3 minutes, and there was statistical significance from 30 seconds to 3 minutes. 4. Oxide layer was formed when titanium is exposed to air, and it was removed when air-powder abraded. It was made when treated with citrate. It is thought that citrate treatment is necessary after the air-powder abrasion, and 1 minute is clinically and qualitatively adequate for burnishing time of citrate.

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Evaluation of Toxicity and Gene Expression Changes Triggered by Quantum Dots

  • Dua, Pooja;Jeong, So-Hee;Lee, Shi-Eun;Hong, Sun-Woo;Kim, So-Youn;Lee, Dong-Ki
    • Bulletin of the Korean Chemical Society
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    • v.31 no.6
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    • pp.1555-1560
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    • 2010
  • Quantum dots (QDs) are extensively employed for biomedical research as a fluorescence reporter and their use for various labeling applications will continue to increase as they are preferred over conventional labeling methods for various reasons. However, concerns have been raised over the toxicity of these particles in the biological system. Till date no thorough investigation has been carried out to identify the molecular signatures of QD mediated toxicity. In this study we evaluated the toxicity of CdSe, $Cd_{1-x}Zn_xS$/ZnS and CdSe/ZnS quantum dots having different spectral properties (red, blue, green) using human embryonic kidney fibroblast cells (HEK293). Cell viability assay for both short and long duration exposure show concentration material dependent toxicity, in the order of CdSe > $Cd_{1-x}Zn_xS$/ZnS > CdSe/ZnS. Genome wide changes in the expression of genes upon QD exposure was also analyzed by wholegenome microarray. All the three QDs show increase in the expression of genes related to apoptosis, inflammation and response towards stress and wounding. Further comparison of coated versus uncoated CdSe QD-mediated cell death and molecular changes suggests that ZnS coating could reduce QD mediated cytotoxicity to some extent only.

An Analysis of Trend and Design Characteristics on the Korea Men's Business Casual (한국 남성 비즈니스 캐주얼의 경향 및 디자인 특성)

  • Kim, Cho-Long;Yum, Hae-Jung
    • Journal of the Korean Society of Costume
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    • v.60 no.10
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    • pp.16-27
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    • 2010
  • Business Casual has been launched because of 'Friday Wear' in the 1990s and in Korea, the business casual has been settled under the influence of the five-day work week. The idea of business casual is that 'Business' and 'Casual wear' are combined. It is the attire that people should wear the jacket for formality and people can wear both on or off duty. As a result of the analysis, based on this, of the characteristics of men's business casual appeared in Seoul collections from 2009 till now, they can be classified in 'Classic & Traditional business casual', 'Modern business casual', 'Natural business casual', 'Feminine business casual' and 'Sporty business casual'. As a result of the analysis of the design characteristics of the Korean men's business casual and the trend, based on the content of the analysis of Seoul Collection, 'Classical Classic & the Traditional', 'Modern of the Contemporary design', 'Natural of the Natural design' and 'the Sporty business casual of the Separate Coordination' including jeans, have presented themselves. Also, as a result of the analysis of Korean business men's business casual according to the classification of the design characteristics in Chapter III, 'Classic & Traditional business casual' account for the majority and then 'Sporty', 'Modern' and 'Natural business casual' account for the rest in order. 'Feminine business casual' hasn't presented itself. As a result of the comparative analysis of the design characteristics in Chapter III & IV, Korean business men's business casual has been concentrated on 'Classical & Traditional' and the design characteristics have not appeared in variety. There has been no such audacious attempt, and items, the characteristics of coordination, colors and materials are not in variety compared to the collection. Meanwhile, as a result of the subsistent characteristics of Koran business men's business casual, the characteristics of business men's business casual can be said to represent themselves in "Separate Coordination with the previous items of formal suits".

A Study of Optimal Operation Policy using Risk Evaluation Criteria(I) (for the Daechung Multi-purpose Reservoir) (위험도 평가기준을 적용한 저수지 최적운영방안 연구(I) (대청댐을 중심으로))

  • Park, Myeong-Gi;Kim, Jae-Han;Jeong, Gwan-Su
    • Journal of Korea Water Resources Association
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    • v.35 no.1
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    • pp.37-49
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    • 2002
  • The application of conventional method for optimizing firm water supply and hydro-electric power generation has some limitation during abnormal or extreme drought periods. Hashimoto et al. (1982) suggested there risk evaluation criteria such as reliability, resilience, and vulnerability. These three criteria have been incorporated into a mixed-integer programming model for evaluating the possible performance of water- supply reservoir (Moy et al., 1986; Srinivasan et al., 1999). However, till now, these kind of researches have been conducted only for water-supply reservoir. Therefore there have been no other study for multi-purpose dam including hydro-electric power generation. This study presents an improved formulation of the previous model for evaluating a multi-purpose reservoir system operation considering water supply and hydro-electric power generation. The modified model was applied to the Daechung multi-purpose reservoir system in the Keum river basin to demonstrate the efficiency of the improved formulation.

A Study on the Changes of the Apartment price in Accordance with Project process of Super high-rise mixed use buildings (초고층 주상복합 건물의 개발사업 단계에 따른 주변지역 아파트가격의 변화에 관한 연구)

  • Kim, Sang Hwan;Choy, Won Cheol;Kim, Ju Hyung;Kim, Jae Jun
    • KIEAE Journal
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    • v.10 no.5
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    • pp.159-164
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    • 2010
  • High-rising buildings are a sort of solution to recent cities. Till now real estate development was concentrated in new development on vacant lots, and it resulted urban sprawl. Generally large cities are confronted with the exodus of industry and population from city. High-rising buildings solve many problems associated with this problem. The purpose of this research is to identify the effect of super high-rise mixed use building project process on apartment price. For this study, the hypothesis is that price of apartments is influenced by project process of super high-rise mixed use building. The study concerned 4 variations of project process that is building permits stage, sale stage, construction starting stage and stage of moving into building. The target projects of buildings are selected by number of floor(over 40 floors) and construction time. And 48 apartment complex are selected around super high-rise mixed use building. This study uses hedonic price function to analysis effect of project process of super high-rise mixed use building. A price of apartments is defined as a dependent variable. Characteristics of residence, complex, district and super high-rise building are defined as independent variables. The results are as follows; first, there is no error in price model of this study. Second, it is found that apartment price was influenced negatively by building permit stage and sale stage of super high-rise mixed use building. But that was influenced positively by construction starting stage and stage of moving into building of that. Third, as the project process of super high-rise mixed use building was proceeded, price of apartments was increased.

Quality and Preference Changes Red Sea Cucumber (Stichopus japonicus) Kimchi during Storage Period (홍해삼 첨가 김치의 저장기간 중 품질 특성 및 기호도 변화)

  • Park, Soo-Yeong;Lim, Hee-Kyoung;Park, Sang-Gyu;Cho, Moon-Jae
    • Journal of Applied Biological Chemistry
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    • v.55 no.2
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    • pp.135-140
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    • 2012
  • To diversify usage of aquacultural red sea cucumber, new Kimchi containing red sea cucumber was developed and its quality and consumer preference were studied. The pH and total acidity, index of Kimchi ripening, decreased till 7 days of storage, then pH change became blunt and finally reached optimum pH 4-4.5. The amount of reducing sugar gradually decreased during storage period. The number of lactic acid bacteria increased in early fermentation period in both Kimchi containing boiled and fresh red sea cucumber, however, more lactic acid bacteria found in fresh red sea cucumber containing Kimchi. Preference of Kimchi containing boiled or fresh red sea cucumber compared to control Kimchi containing fermented salted fish was evaluated. The results revealed there were no differences in color, flavor, texture, taste, after taste and overall preference.

Classification and Characteristics of Annual Bluegrass(Poa Annua L.) Collected from Golf Courses in Korea (우리 나라에서 수집한 새포아풀의 분류 및 특성)

  • 태현숙;신동현;김길웅;신홍균
    • Proceedings of the Turfgrass Society of Korea Conference
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    • 2002.02a
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    • pp.3-5
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    • 2002
  • This study was carried out to get better understandings about morphological, ecological, and genetical characteristics of annual bluegrass collected from different golf courses in Korea and eventually to establish a successful control strategy. Twenty five local lines of annual bluegrass collected from 20 golf courses in Korea were classified into annual or perennial type on the basis of morphological characteristics. Twelve local lines showing obvious morphological differences were selected and then genetically assessed using RAPD analysis. Classification of the 12 local lines through RAPD analysis were considerably similar to that determined by both of morphological differences and phenotype. Responses of the two types of annual blugrass to herbicides were also examined. Shoot growth of annual bluegrass was significantly suppressed by flazasulfuron and the annual type was more susceptible than perennial type, regardless of flazasulfuron concentrations used. By pendimethalin treatment, there was no clear difference in susceptibility between the two types of annual bluegrass. However, by the treatment of dithiopyr, annual type was more sensitive than perennial type in both shoot and root growth. Nine tree species were screened to detect their allelopathic potential on turfgrasses and annual bluegrass. Acacia (Robinia pseudo-acacia) leaves showed selective inhibition in the shoot and root growth as well as their seed germination when treated with 2% and 10%(v/v) of the extract. However, the other leaf extracts except acacia inhibited non-selectively the growth of three turfgrass species such as bentgrass, perennial ryegrass and zoysiagrass and annual bluegrass. The PAL activities of annual bluegrass increased at 24 h after treatment of acacia leaf extract and peaked at 36 h and then decreased till 60h. The highest PAL activity was observed at 36h after treatment of 10%. The highest activity of CA4H in annual bluegrass was observed at 2h after treatment of acacia extract and the level was 4 times greater than that of the control. The phenolic acids such as p-coumaric acid, salicylic acid and ferulic acid were increased with the treatment of acacia leaf extract. The chloroplast membrane and cell wall of annual bluegrass were destroyed by treatment of acacia leaf extract and its inner materials were released. The membranes in annual bluegrass cells might be destroyed by phytotoxic compounds from acacia leaf extract.

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