• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.356-363
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• 2016

Human papillomavirus (HPV), a non-enveloped, double-stranded DNA tumor virus, is a primary etiological agent of cervical cancer development. As a potential tool for prophylactic vaccination, the development of virus-like particles (VLPs) containing the HPV16 L1 capsid protein is highly desired. In this study, we developed a high-level expression system of the HPV16 L1 in Escherichia coli for the purpose of VLP development. The native gene of HPV16 L1 has many rare codons that cause the early termination of translation and result in the production of truncated forms. First, we optimized the codon of the HPV16 L1 gene to the preferable codons of E. coli, and we succeeded in producing the full-size HPV16 L1 protein without early termination. Next, to find the best host for the production of HPV16 L1, we examined a total of eight E. coli strains, and E. coli BL21(DE3) with the highest yield among the strains was selected. With the selected host-vector system, we did a fed-batch cultivation in a lab-scale bioreactor. Two different feeding solutions (complex and defined feeding solutions) were examined and, when the complex feeding solution was used, a 6-fold higher production yield (4.6 g/l) was obtained compared with that with the defined feeding solution.

• Biomolecules & Therapeutics
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• v.30 no.5
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• pp.418-426
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• 2022

Chimeric antigen receptor T (CAR-T) cell therapy is one of the promising anticancer treatments. It shows a high overall response rate with complete response to blood cancer. However, there is a limitation to solid tumor treatment. Additionally, this currently approved therapy exhibits side effects such as cytokine release syndrome and neurotoxicity. Alternatively, bispecific antibody is an innovative therapeutic tool that simultaneously engages specific immune cells to disease-related target cells. Since programmed death ligand 1 (PD-L1) is an immune checkpoint molecule highly expressed in some cancer cells, in the current study, we generated αCD3xαPD-L1 bispecific antibody (BiTE) which can engage T cells to PD-L1⁺ cancer cells. We observed that the BiTE-bound OT-1 T cells effectively killed cancer cells in vitro and in vivo. They substantially increased the recruitment of effector memory CD8⁺ T cells having CD8⁺CD44⁺CD62L^low phenotype in tumor. Interestingly, we also observed that BiTE-bound polyclonal T cells showed highly efficacious tumor killing activity in vivo in comparison with the direct intravenous treatment of bispecific antibody, suggesting that PD-L1-directed migration and engagement of activated T cells might increase cancer cell killing. Additionally, BiTE-bound CAR-T cells which targets human Her-2/neu exhibited enhanced killing effect on Her-2-expressing cancer cells in vivo, suggesting that this could be a novel therapeutic regimen. Collectively, our results suggested that engaging activated T cells with cancer cells using αCD3xαPD-L1 BiTE could be an innovative next generation anticancer therapy which exerts simultaneous inhibitory functions on PD-L1 as well as increasing the infiltration of activated T cells having effector memory phenotype in tumor site.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.14 no.3
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• pp.31-37
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• 2015

In this paper, newly proposed dual-band microstrip antennas using stacked inverted-L-shaped parasitic elements are presented for GPS $L_1(1.575GHz)$ and $L_2(1.227GHz)$ bands. For making dual band which has large interval, ${\lambda}/4$($L_1$ band) inverted-L-shaped parasitic elements were stacked at both side of radiation apertures on the half-wavelength($L_2$ band) patch antennas. The resonance in the parasitic elements occurs through coupling to the patch. Next, due to using circular polarization at GPS, ${\lambda}/4$($L_1$ band) inverted-L-shaped parasitic elements was stacked using sequential rotation technique on the patch and both side of the diagonal corners of the antenna were eliminated to make dual-band circular polarization. The designed circular polarized antenna's dimensions are $0.43{\lambda}L{\times}0.43{\lambda}L{\times}0.06{\lambda}L$ (${\lambda}L$ is the free-space wavelength at 1.227 GHz). Measured -10 dB bandwidths was 120 MHz(7.6%) and 82.5 MHz(6.7%) at GPS $L_1$ and $L_2$ bands. and 3 dB axial ration bandwidths are 172 MHz(10.9%) and 25 MHz(2.03%), respectively. All of these cover the respective required system bandwidths. Within each of the designed bands, broadside radiation patterns were observed.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.14 no.5
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• pp.38-43
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• 2015

In this paper, we present novel dual-band microstrip antennas using inverted-L-shaped parasitic elements vertically at radiation apertures for GPS L1(1.575 GHz) and L2(1.227 GHz) bands. For making dual band which has large interval, the inverted-L-shaped parasitic element was loaded at the radiation aperture of a half-wavelength patch antenna(GPS L1) in opposite direction of the feeding point for receiving the low frequency(GPS L2). The low frequency occurs by perturbation and coupling between the patch and parasitic. Next, due to use circular polarizations at the GPS applications, two inverted-L-shaped parasitic elements were loaded at radiation apertures of each polarizations and the feeding point was moved at diagonal part of the patch. The dimensions of the designed circularly polarized antenna were $88.5{\times}79{\times}10.4mm^3$ ($0.36{\lambda}L{\times}0.32{\lambda}L{\times}0.04{\lambda}L$, ${\lambda}L$ is the free-space wavelength at 1.227 GHz). Measured -10 dB bandwidths were 116.3 MHz(7.4%) and 64.3 MHz(5.2%) at GPS L1 and L2 bands, respectively. All of these cover the respective required system bandwidths. The measured 3 dB axial ratio bandwidths were 11.7 MHz(0.74%) and 14 MHz(1.14%), respectively. Within each of the designed bands, broadside radiation patterns were observed.

• Journal of the Chungcheong Mathematical Society
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• v.10 no.1
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• pp.43-56
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• 1997

In this paper, we introduce a new class of ${\delta}$-frames and study its properties. To do so, we introduce ${\delta}$-filters, almost Lindel$\ddot{o}$f frames and Lindel$\ddot{o}$f frames. First, we show that a complete chain or a complete Boolean algebra is a ${\delta}$-frame. Next, we show that a ${\delta}$-frame L is almost Lindel$\ddot{o}$f iff for any ${\delta}$-filter F in L, ${\vee}\{x^*\;:\;x{\in}F\}{\neq}e$. Last, we show that every regular Lindelof ${\delta}$-frame is normal and a Lindel$\ddot{o}$f ${\delta}$-frame is preserved under a ${\delta}$-isomorphism which is dense and codense.

• Journal of the Korean Society of Costume
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• v.53 no.2
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• pp.119-125
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• 2003

This study shows the dyeability and antibacterial activity about cotton and silk fabrics dyed with Chrysanthem indicum L. In the case of dyeability, in the first, K/S value was shown comparatively high numerical value. The value of all test sample was over 20. In the next, in the case of colorfastness, the light colorfastness showed the lowest grade, the other colorfastness was relatively good. However there are some differences according to fabrics and mordants. In the case of antibacterial activity to Klebsielia pneumoniae, the cotton fabrics had the most antibacterial effect at the dyed fabrics that had been mordanted $CuSO_4$.$5H_2O$, and the silk fabrics had the most antibacterial effect at the dyed fabrics that had been mordanted $CuSO_4$.$5H_2O$.$Al_2$ and $(SO_4)_3$.

• Applied Microscopy
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• v.24 no.1
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• pp.1-10
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• 1994

The integumental secretory structure is exocrine unicellular gland located in the epidermis of goldfish, Carassius auratus, and divided into two groups, mucous and granular cells. By the histochemical studies of integumental secretions the mucos cells reacted for acidic polysaccharides, and the granular cells for neutral glycoprotein. According to concentration of the secretion the integumental mucous are gradually sulphated. The mucous cells are typical form of goblet cell located in the upper region of the epidermis, and membrane bounded vesicles of the mucous are observed several size and electron densities by the cellular differentiation. The granular cells in middle and lower epidermis are present syncitial forms occasionally, and contain electron dense granules sized $1.0{\mu}m$ which are accumulated in cytoplasmic process held the cells to the basal lamina. The precursors of the integumental secretory materials are originated from the rough endoplasmic reticulum and next transported through the Golgi apparatus as a form of membrane bounded vesicles. After accomplish this process mature secretions are extruded to integumental surface by the mechanism of merocrine secretion in response to nerve stimulations respectively.

• Korean Journal of Logic
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• v.11 no.1
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• pp.131-156
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• 2008

This paper first investigates several uninorm logics (introduced by Metcalfe and Montagna in [8]) as fuzzy-relevance logics. We first show that the uninorm logic UL and its extensions IUL, UML, and IUML are fuzzy-relevant; fuzzy in Cintula's sense, i.e., the logic L is complete with respect to linearly ordered L-matrices; and relevant in the weak sense that ${\Phi}{\rightarrow}{\Psi}$ is a theorem only if either (i) $\Phi$ and $\Psi$ share a sentential variable or constant, or (ii) both $\sim\Phi$ and $\Psi$ are theorems. We next expand these systems to those with $\triangle$.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.1-10
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• 2005

Parthenogenesis in mulberry silkworm, Bombyx mori L. acquires immense use in the development of outstanding homozygous lines with higher viability, hybrid vigour, combining ability and less phenotypic variability. It can serve as a powerful tool in controlling sex of the offsprings as well as a useful tool in selection. In fact India is the second largest silk producing country in the world next only to China and all the five types of natural silks viz., mulberry, oak tasar, tropical tasar, muga and eri are produced in India. However, little information is available on the role of artificial parthenogenesis in the development of superior silkworm breeds. This paper overviews some important studies carried out on artificial parthenogenesis, and outline of different types of parthenogenesis, methods of induction of artificial parthenogenesis, factors responsible for successful parthenogenetic development, cytogenetics of artificial parthenogenesis and role of artificial parthenogenesis in silkworm breeding. Besides, an attempt is made to describe briefly about parthenogenetic engineering which includes cloning in silkworm, artificial insemination, chimeras, hybridization, chromosomal substitution and recombinant DNA in silkworm.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.333-337
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• 1997

Pods and kernels of two peanut(Arachis hypogaea L.) cultivars, a Shinpung type, Shindaekwangtangkong and a Virginia bunch type, Namdaetangkong were harvested periodically after the gynophore earthing in the field condition and their developments were monitored by observation of the changes in size, weight and moisture content. Seed germinability and seedling vigor were tested to set up a method for hastening the generation advancement in breeding procedure. Pods and kernels reached the maximum in size at 30 to 40 days after gynophore earthing and at that time the kernels had one hundred percent germinability and the seedlings sprouted from those kernels exhibited practically acceptable viability enough to grow for the next generation.