• Title/Summary/Keyword: Neutral red

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Effect of Red Pepper Varieties on the Microflora, Enzyme Activities and Taste Components of Traditional Kochujang during Fermentation (고추품종을 달리한 전통식 고추장의 숙성중 미생물, 효소활성 및 맛성분의 변화)

  • Shin, Dong-Hwa;Kim, Dong-Han;Choi, Ung;Lim, Mi-Sun;An, Eun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.6
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    • pp.1050-1057
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    • 1997
  • Microbial counts, enzyme activities and taste components of traditional kochujangs prepared with the powders from 4 different varieties of red pepper, were investigated during 90 days fermentation for the industrial production of traditional kochujang. The viable cell counts of anaerobic bacteria in kochujangs did not change remarkably during fermentation, however, aerobic bacterial counts showed a rapid increase up to 90 days of aging. The yeasts in all kochujang samples increased until 60 days of aging and than decreased. After 90 days of aging, the count of aerobic bacteria in Kumtop kochujang was higher than those of others. The activities of liquefying amylase decreased during the aging, but those of saccharogenic amylase increased at 60 days of aging. The activities of neutral protease were higher than those of acidic protease, and increased during the middle and last period of aging. The major free sugars of kochujang were maltose and glucose, and their contents were higher in Hongkwang kochujang. The major organic acids of kochujang were succinic, formic and citric acid, followed by lactic acid. Succinic acid content in kochujang decreased during fermentation, whereas formic and citirc acids were increased. The major free amino acids were serine, proline, glutamic acid, aspartic acid and alanine. Kumtop kochujang contained the highest amount of total free amino acids. Among the nucleotides and related components in kochujang, cytidine-5-monophosphate was the most abundant component at the begining of aging period, while hypoxanthine increased remarkably during fermentation. Hanwang kochujang was higher in the content of nucleotides than others. Capsaicin contents of kochujang decreased during aging and those of Jangter kochujang was higher than that of others. Sensory evaluation showed that Jangter kochujang was significantly better than Kumtop kochujang in overall acceptability, but there were no appreciable differences in color and flavor.

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Rumen fermentation, methane production, and microbial composition following in vitro evaluation of red ginseng byproduct as a protein source

  • Hamid, Muhammad Mahboob Ali;Moon, Joonbeom;Yoo, Daekyum;Kim, Hanbeen;Lee, Yoo Kyung;Song, Jaeyong;Seo, Jakyeom
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.801-811
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    • 2020
  • The main objective of this in vitro study was to evaluate red ginseng byproduct (RGP) as a protein resource and its effects on rumen fermentation characteristics, microflora, CO2, and CH4 production in ruminants. Four treatments for in vitro fermentation using buffered rumen fluid over a 48 h incubation period were used: 1, RGP; 2, corn gluten feed (CGF); 3, wheat gluten (WG); and 4, corn germ meal. In vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro crude protein digestibility (IVCPD), volatile fatty acids, pH, and ammonia nitrogen (NH3-N) were estimated after 48 h incubation. Gas production was investigated after 3, 6, 12, 24, 36 and 48 h. The CO2 and CH4 were evaluated after 12, 24, 36, and 48 h. A significant difference in total gas production and CO2 emissions was observed (p < 0.01) at all incubation times. CH4 production in RGP were higher (p < 0.05) than that in other treatments but a higher CH4 portion in the total gas production was observed in WG (p < 0.05) at 48 h incubation. The IVDMD, IVNDFD, and IVCPD of RGP was lower than those of other conventional ingredients (p < 0.01). The RGP had the lowest NH3-N value among the treatments (p < 0.01). The RGP also had the lowest total VFA concentration (p < 0.01), but presented the highest acetate proportion and acetate to propionate ratio among the treatments (both, p < 0.01). The abundance of Prevotella ruminicola was higher in RGP than in WG (p < 0.01), whereas RGP has lower methanogenic archaea (p < 0.01). In conclusion, based on the nutritive value, IVDMD, low NH3-N, and decreased methanogenic archaea, RGP inclusion as a protein source in ruminant diets can be an option in replacing conventional feed sources.

Fabric Dyeing with Lichen Parmotrema austrosinence and Improvement of Dyeability by Chitosan Treatment (Parmotrema austrosinence(지의류)를 이용한 직물염색과 키토산 처리에 의한 염색성 향상)

  • Yoo, Hye-Ja;Lee, Hye-Ja;Rhie, Jeon-Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.32 no.6
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    • pp.882-889
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    • 2008
  • Three fabrics, 100% silk, nylon and cotton each, were dyed with a lichen dye solution prepared by a fermentation method under conditions of varying dyebath pH and temperature. To verify the effect of chitosan on fabric dyeing, the 100% cotton fabric was treated with a chitosan solution before dyeing. The K/S, CIE $L^*$, $a^*$, $b^*$, ${\Delta}E$ and Munsell values of the dyed samples were measured. Colorfastness of each sample was also investigated. The maximum K/S value was measured at 520nm wavelength for the dyed silk fabric and at 480nm for the dyed cotton and nylon. The K/S values for the dyed silk fabric were much greater than those of the other fabrics. The dyed silk fabric showed a red tone on the Munsell color system, and the dyed nylon and cotton fabrics a yellowish red tone. Dye affinity to fabrics was better in a neutral or acidic dyebath. As dyeing temperature increased, K/S values increased for the dyed nylon and cotton fabrics but not for the silk. Dyeability of cotton fabrics could improve by Chitosan treatment. As for most natural dyes, colorfastness of all dyed samples was poor. The silk fabric showed an excellent dry cleaning fastness of Grade 5.

The Morphology, Physical and Chemical Characteristics of the Red-Yellow Soils in Korea (우리나라 전토양(田土壤)의 특성(特性) (저구릉(低丘陵), 산록(山麓) 및 대지(臺地)에 분포(分布)된 적황색토(赤黃色土)를 중심(中心)으로))

  • Shin, Yong Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.1
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    • pp.35-52
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    • 1973
  • Red Yellow Soils occur very commonly in Korea and constitute the important upland soils of the country which are either presently being cultivated or are suitable for reclaiming and cultivating. These soils are distributed on rolling, moutain foot slopes, and terraces in the southern and western parts of the central districts of Korea, and are derived from granite, granite gneiss, old alluvium and locally from limestone and shale. This report is a summary of the morphology, physical and chemical characteristics of Red Yellow Soils. The data obtained from detailed soil surveys since 1964 are summarized as follows. 1. Red-Yellows Soils have an A, Bt, C profile. The A horizon is dark colored coarse loamy or fine loamy with the thin layer of organic matter. The B horizon is dominantly strong brown, reddish brown or yellowish red, clayey or fine loamy with clay cutans on the soil peds. The C horizon varies with parent materials, and is coarser texture and has a less developed structure than the Bt horizon. Soil depth, varied with relief and parent materials, is predominantly around 100cm. 2. In the physical characteristics, the clay content of surface soil is 18 to 35 percent, and of subsoil is 30 to 90 percent nearly two times higher than the surface soil. Bulk density is 1.2 to 1.3 in the surface soil and 1.3 to 1.5 in the subsoil. The range of 3-phase is mostly narrow with 45 to 50 percent in solid phase, 30 to 45 percent in liquid one, and 5 to 25 percent in gaseous state in the surface soil; and 50 to 60 solid, 35 to 45 percent liquid and less than 15 percent gaseous in the subsoil. Available soil moisture capacity ranges from 10 to 23 percent in the surface soil, and 5 to 16 percent in the subsoil. 3. Chemically, soil reaction is neutral to alkaline in soils derived from limestone or old fluviomarine deposits, and acid to strong acid in other ones. The organic matter content of surface soil varying considerably with vegetation, erosion and cultivation, ranges from 1.0 to 5.0 percent. The cation exchange capacity is 5 to 40 me/100gr soil and closely related to the content of organic matter, clay and silt. Base saturation is low, on the whole, due to the leaching of extractable cations, but is high in soils derived from limestone with high content of lime and magnesium. 4. Most of these soils mainly contain halloysite (a part of kaolin minerals), vermiculite (weathered mica), and illite, including small amount of chlorite, gibbsite, hematite, quartz and feldspar. 5. Characteristically they are similar to Red Yellow Podzolic Soils and a part of Reddish Brown Lateritic Soils of the United States, and Red Yellow Soils of Japan. According to USDA 7th Approximation, they can be classified as Udu Its or Udalfs, and in FAO classification system to Acrisols, Luvisols, and Nitosols.

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Characterization of the Strong Proteolytic Bacteria Isolated from Low Salt Fermented Anchovy and of Protease Produced by that Strain (저식염멸치젓에서 분리한 단백질분해력이 강한 세균 및 생산된 단백분해효소의 특성)

  • CHA Yong-Jun;LEE Eung-Ho;LEE Kang-Hee;CHANG Dong-Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.2
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    • pp.71-79
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    • 1988
  • For the purpose of producing low salt fermented anchovy by accelerated method with a strong proteolytic bacteria, in this study, a strong proteolytic bacterium was isolated from low salt fermented anchovy and its bacteriological characteristics and properties of protease were experimented. The results obtained were as fellows : three proteolytic bacteria, Aeromonas anaerogenes Barillus subtilis and Staphylococcus saprophyticus were isolated from low salt fermented anchovy($4\%\;of\;salt,\;4\%\;of\;KCl,\;0.5\%\;of\;lactic\;acid,\;6\%$of sorbitol and $4\%$ of alcohol extract of red pepper) after 40 days fermentation. Among these strains, which grow best at $30^{\circ}C$, pH 7.0, B. subtilis was found the best proteolytic strain and benefit for industrial use as shown $0.95\;hr^{-1}$ of specific growth rate, $89{\mu}g-Tyr/hr.ml$ of maximum activity after 12 hrs culture in TPY broth. The protease produced by by B. subtilis showed maximum activity at $35^{\circ}C$, pH 7.0, and molecular weight was estimated to be 23,000 by Sephadex G-100 filtration, and it was supposed to be a kind of metal chelator sensitive neutral protease from the results of strong sensitivity against EDTA, o-phenanthroline and metal ions such as $Cu^{2+},\;Ni^{2+},\;Fe^{2+}.Km$ value of that by method of Lineweaver-Burk was determinded to be $0.73\%$ for casein as a substrate.

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The Effect of Distinctiveness of stimulus and Partial Retrieval on Memory (자극의 구별성과 부분 인출이 기억에 미치는 영향)

  • Jung, Yoonjae
    • Korean Journal of Cognitive Science
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    • v.30 no.1
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    • pp.31-50
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    • 2019
  • The present study is designed to investigate the effect of perceptive, emotional and semantic distinctiveness on retrieval-induced forgetting(RIF). Experiment 1 was designed to construct a category and category list for RIF experimental paradigm and to investigate the effects of perceptual distinctness on retrieval-induced forgetting. It was used for the list consisting of the six categories and six words in each category list. In controlled conditions, all the stimuli were presented in black and Gothic. In contrast, perceptual distinctiveness conditions, half of the category list were presented in red and Gungseoche. RIF was observed in all conditions. Experiment 2 was designed to investigate the effects of semantic and emotional distinctiveness on retrieval-induced forgetting. In neutral conditions, adjectives related to items were added. In the emotional distinctiveness condition, half of the items in the category were manipulated in such a way as to add the negative adjectives. In the semantic distinctiveness condition, half of the items in the category were manipulated in such a way as to add the inappropriate adjective. As a result, RIF occurred in the neutral condition, but RIF did not occur in both the emotional discrimination condition and the semantic discrimination. These results suggest the possibility that the RIF will not occur when the distinctiveness occurs within a categorical relationship.

Growth of Red-leaf Lettuce and Changes in Soil Solution Chemical Properties of Coir-dust Containing Root Media Influenced by Application Rates of Pre-planting Fused-Superphosphate (코이어 더스트 혼합상토에 용과린의 시비수준에 의한 적축면 상추의 생장과 근권부 화학성 변화)

  • Kim, Chang Hyeon;Choi, Jong Myung;Lee, Dong Hoon
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.658-667
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    • 2015
  • This research was conducted to investigate the influence of various levels of fused superphosphate as pre-planting fertilizer on the growth of red-leaf lettuce and changes in the chemical properties of the soil solution in three root media, namely coir-dust plus expanded rice hull (8:2, v/v; CD+ERH), carbonized rice hull (6:4; CD+CRH), or ground and aged pine bark (8:2; CD+GAPB). The amounts of fused superphosphate (FSP) incorporated into the three root media during formulation were controlled from 0 to $6.0g{\cdot}L^{-1}$ in $1.5g{\cdot}L^{-1}$ increments. The root media containing fertilizers were packed into 300 mL plastic pots and seedlings of red-leaf lettuce at the 3rd leaf stage were transplanted. After transplanting, the crops were fed with a solution of neutral fertilizer ($100mg{\cdot}L^{-1}$). The growth of red-leaf lettuce was investigated 5 weeks after transplanting and soil solutions were extracted and analyzed every week for pH, EC, and concentrations of macro-nutrients. The elevation of application rates of FSP in the three root media resulted in better growth, and the crops grown in CD+ERH and CD+GRPB had greater fresh and dry weights than those in CD+CRH when compared among the treatments of equal amounts of FSP. The pH and $PO_4{^{-3}}$ concentrations in the soil solution of CD+CRH at 3 weeks after transplant were in the ranges of 4.0 to 4.8 and 20 to $100mg{\cdot}L^{-1}$, respectively. These were lower pH and higher $PO_4{^{-3}}$ concentrations than those in CD+ERH and CD+GAPB. The $K^+$ concentrations were higher in CD+CRH than those in the other two root media, and the elevation of FSP application rates resulted in higher $Ca^{+2}$, $Mg^{+2}$ and $SO_4{^{-2}}$ concentrations in soil solution of the three root media. The $NO_3$-N concentrations in soil solution rose continuously during crop cultivation, implying that the leaching percentage was elevated. The soil solution EC varied, showing the same tendencies as the $NO_3$-N concentrations. The above results indicated that the CD+ERH and CD+GRPB media performed better than CD+CRH, and optimum application rates of FSP in the three root media were 4.5 to $6.0g{\cdot}L^{-1}$ for pot cultivation of red-leaf lettuce.

Comparison of Forage Yield and Quality of Forage Legume (콩과목초의 사초 수량 및 품질 비교)

  • Kim, J.D.;Kim, S.G.;Kwon, C.H.
    • Journal of Animal Science and Technology
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    • v.46 no.3
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    • pp.437-442
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    • 2004
  • Forage legumes are being used as an important source of protein for dairy farm as well as nitrogen fertilizer. This experiment was conducted to evaluate forage production and quality of five legumes at Cheonan Yonam College Experimental Livestock Farm for 1 year(2001-2002). Five legumes were crimson clover (Trifolium incarnatum L.) 'Tibbee', hairy vetch(Vicia villosa Roth) 'Common', red clover(Trifolium pratense L.) 'Kenland', persian clover(Trifolium resupinatum L.) 'Leeton', alfalfa(Medicago sativa L.) 'Vernal'. All legumes were shown cold hardiness of 8 rating or higher except crimson clover(7 rating). Flowering stage was observed 23th of April for crimson clover, 13th of May for hairy vetch, 3rd of May for red clover, and 12th of May for alfalfa. But persian clover didn't at harvest. Dry rnatter(DM) of crimson clover(17.6%) was the highest among legumes. In DM and total digestible nutrients(TDN) yields, crimson clover and hairy vetch were higher than those of other legumes. The crude protein of hairy vetch(20.5%) was the highest among legumes. In neutral detergent fiber(NDF) and acid detergent fiber(ADF), persian clover was the lowest(35.0% and 25.0%). Calculations of relative feed value(RFV) based on NDF and ADF of legumes were over 125(Prime degree) except hairy vetch. RFV and TDN of persian clover were the highest among legumes. According to the results of this study, crimson clover and hairy vetch are excellent in forage yield. Persian clover is superior in forage quality among legumes.

Emotional reactions of users for the skin-tone variations of portrait photography (인물 사진의 피부 톤 변화에 대한 감성 반응)

  • Kim, Eunyoung;Park, Chongwook;Woo, Sungju
    • Science of Emotion and Sensibility
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    • v.17 no.1
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    • pp.105-114
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    • 2014
  • The skin-tone in portrait photography is one of the most sensitive parts that need retouching by a photographer. To investigate the emotional reactions related to users' preferences for the skin-tone variations in portrait photography, two experiments were conducted. The first experiment included JND(Just Noticeable Difference) test to determine the general distribution of preferences with many photographs that varied in phases up to an extreme one, as a result, preferences for the brightest skin-tone and the red or magenta one were found. Based on the first experiment, we reduced the number of samples by adjusting their brightness to the brightest phase constantly. To intensify the second experiment, we reduced the number of the other colored samples to only one and made samples for five phases from green to magenta, namely the most preferred skin-tone in the first experiment. In the second experiment, the common preference for a neutral skin-tone and the partial difference between the two gender groups were found. In conclusion, the users' preference for a particular skin-tone was positively affected by emotions such as 'happiness' or 'comfortable'. With this investigation, we compiled some statistically meaningful facts to confirm that the preferences of the users depend positively on controlling the skin-tone in portrait photography.

Effects of Taeeumjowuitanggagam-bang on the Expression of Adipocytokines and SREBPs in Liver of db/db Mouse (태음조위탕가감방(太陰調胃湯加減方)의 db/db 마우스 간(肝)에 대한 아디포사이토카인 및 SREBPs의 발현에 미치는 영향)

  • Baek, Jong-Woo;Jeong, Mi-Kyung;Ko, Seong-Gyu;Jun, Chan-Yong;Park, Jong-Hyeong;Choi, You-Kyung
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.6
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    • pp.989-995
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    • 2010
  • The aim of this study was to investigate the effect of Taeeumjowuitanggagam-bang(TJV) on the mRNA expression of adipocytokines (TNF-${\alpha}$ and IL-6), and SREBPs. Mice were divided into 4 groups ; a normal group of db/+ mice, a control group of db/db mice, a group (db/db mice) treated with TJV 200 mg/kg, and a group (db/db mouse) treated with TJV 500 mg/kg. They were treated orally with TJV and measured their body weight every other day during 9 weeks. After that, we measured the mRNA expression of adipocytokines (TNF-${\alpha}$ and IL-6) and SREBPs (SREBE-1a and SREBP-1c) in liver, and blood concentration of total cholesterol, triglyceride, too. In addition, liver samples were fixed in 4% PFA for 2 hours and stored in $-70^{\circ}C$. Liver tissues were embedded in Optical cutting temperature(OCT) compound and 30 ${\mu}m$ sections were cut. Tissue sections were stained Oil-Red-O to visualize neutral lipids. Nuclei were stained with hematoxylin solution. In result, the TJV reduced the mRNA expressions of TNF-${\alpha}$ and IL-6 and SREBPs in liver and stained liver tissue less red than control group. However, there was no significant differences in total cholestreol and triglyceride blood concentration and body weight among groups. The TJV has inhibitory effect on the mRNA expression of adipocytokines and SREBPs. Therefore, it is assumed that the TJV is related to inhibiting lipogenesis in the liver