Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Taeeumjowuitanggagam-bang on the Expression of Adipocytokines and SREBPs in Liver of db/db Mouse

태음조위탕가감방(太陰調胃湯加減方)의 db/db 마우스 간(肝)에 대한 아디포사이토카인 및 SREBPs의 발현에 미치는 영향

  • Baek, Jong-Woo (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Jeong, Mi-Kyung (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Ko, Seong-Gyu (Preventive Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Jun, Chan-Yong (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Park, Jong-Hyeong (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Choi, You-Kyung (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University)
  • 백종우 (경원대학교 한의과대학 내과학교실) ;
  • 정미경 (경원대학교 한의과대학 내과학교실) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실) ;
  • 전찬용 (경원대학교 한의과대학 내과학교실) ;
  • 박종형 (경원대학교 한의과대학 내과학교실) ;
  • 최유경 (경원대학교 한의과대학 내과학교실)
  • Received : 2010.10.25
  • Accepted : 2010.11.10
  • Published : 2010.12.25
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Abstract

The aim of this study was to investigate the effect of Taeeumjowuitanggagam-bang(TJV) on the mRNA expression of adipocytokines (TNF-${\alpha}$ and IL-6), and SREBPs. Mice were divided into 4 groups ; a normal group of db/+ mice, a control group of db/db mice, a group (db/db mice) treated with TJV 200 mg/kg, and a group (db/db mouse) treated with TJV 500 mg/kg. They were treated orally with TJV and measured their body weight every other day during 9 weeks. After that, we measured the mRNA expression of adipocytokines (TNF-${\alpha}$ and IL-6) and SREBPs (SREBE-1a and SREBP-1c) in liver, and blood concentration of total cholesterol, triglyceride, too. In addition, liver samples were fixed in 4% PFA for 2 hours and stored in $-70^{\circ}C$. Liver tissues were embedded in Optical cutting temperature(OCT) compound and 30 ${\mu}m$ sections were cut. Tissue sections were stained Oil-Red-O to visualize neutral lipids. Nuclei were stained with hematoxylin solution. In result, the TJV reduced the mRNA expressions of TNF-${\alpha}$ and IL-6 and SREBPs in liver and stained liver tissue less red than control group. However, there was no significant differences in total cholestreol and triglyceride blood concentration and body weight among groups. The TJV has inhibitory effect on the mRNA expression of adipocytokines and SREBPs. Therefore, it is assumed that the TJV is related to inhibiting lipogenesis in the liver

Keywords

References

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