• BMB Reports
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• v.49 no.1
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• pp.3-10
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• 2016

microRNAs (miRNAs) are key regulators of cell state transition and retention during stem cell proliferation and differentiation by post-transcriptionally downregulating hundreds of conserved target genes via seed-pairing in their 3' untranslated region. In embryonic and adult stem cells, dozens of miRNAs that elaborately control stem cell processes by modulating the transcriptomic context therein have been identified. Some miRNAs accelerate the change of cell state into progenitor cell lineages—such as myoblast, myeloid or lymphoid progenitors, and neuro precursor stem cells—and other miRNAs decelerate the change but induce proliferative activity, resulting in cell state retention. This cell state choice can be controlled by endogenously or exogenously changing miRNA levels or by including or excluding target sites. This control of miRNA-mediated gene regulation could improve our understanding of stem cell biology and facilitate their development as therapeutic tools. [BMB Reports 2016; 49(1): 3-10]

• Journal of Integrative Natural Science
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• v.6 no.1
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• pp.57-63
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• 2013

Histone deacetylase (HDACs) is an enzyme family that deacetylates histones and non-histones protein. Availability of crystal structure of HDAC8 has been a boosting factor to generate target based inhibitors. Hydroxamic class is the most studied one to generate potent inhibitors. HDAC class I and class II enzymes are emerging as a therapeutic target for cancer, diabetes, inflammation and other diseases. DNA methylation and histone modification are epigenetic mechanism, is important for the regulation of cellular functions. HDACs enzymes play essential role in gene transcription to regulate cell proliferation, migration and death. The aim of this article is to provide a comprehensive overview about structure and function of HDACs enzymes, histone deacetylase inhibitors (HDACi) and HDACs enzymes as a therapeutic target for cancer, inflammation and diabetes.

• Proceedings of the KSRS Conference
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• 2000.04a
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• pp.9-9
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• 2000

For the planning of future land use for economic activities, an essential component is the identification of the vulnerable areas for natural hazard and environmental impacts from the activities. Also, exploration for mineral and energy resources is carried out by a step by step approach. At each step, a selection of the target area for the next exploration strategy is made based on all the data harnessed from the previous steps. The uncertainty of the selected target area containing undiscovered resources is a critical factor for estimating the exploration risk. We have developed not only spatial prediction models based on adapted artificial intelligence techniques to predict target and vulnerable areas but also validation techniques to estimate the uncertainties associated with the predictions. The prediction models will assist the scientists and decision-makers to make two critical decisions: (i) of the selections of the target or vulnerable areas, and (ii) of estimating the risks associated with the selections.

• Archives of Pharmacal Research
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• v.25 no.6
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• pp.889-894
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• 2002

Acharan sulfate (AS) is a glycosaminoglycan (GAG) prepared from the giant African snail, Achatina fulica. In this study, some biological activities of AS were evaluated on the basis of structural similarities to heparin/heparan sulfate and the biological functions of GAGs. We demonstrated that it exhibited strong immunostimulating activities as measured by carbon clearance test in mice and in vivo phagocytosis. It also exhibited a significant hypoglycemic activity in epinephrine (EP)-induced hyperglycemia as well as antifatigue effects by weight-loaded forced swimming test. And it showed hypolipidemic activities in cholesterol-rich mixture induced hyperlipidemia in rats. The above results indicate that AS has diverse biological activities and suggest therapeutically important target molecules.

• YAKHAK HOEJI
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• v.43 no.3
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• pp.334-341
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• 1999

Thyroid peroxidase is a biochemical target protein for the antithyroid drugs. Ethanol extracts from one hundred and thirty seven natural products were screened for the inhibition of thyroid peroxidase activity. Thyroid peroxidase was purified from porcine thyroids, and the inhibition of peroxidase activity was evaluated using guaiacol oxidation (C-C coupling) assay. Twenty one natural products expressed a remarkable inhibition (>50%) of peroxidase activity at $330{\mu\textrm{g}}$ solid weight/m. The 50% inhibitory concentration ($IC_{50}$) of 70% ethanol extract from six potent natural products ranged from 3.1 to $31.2{\;}{\mu\textrm{g}}$ solid weight/m, in contrast to the range ($0.33~0.54{\;}{\mu\textrm{g}}/ml$) of $IC_{50}$ values fro catechin and epigallocatechin gallate as positive controls. Noteworthy, the extract of Camellia taliensis showed irreversible inhibition of the enzyme. It is suggested that extract from some natural products such as Camellia taliensis, Rheum undulatum or Euphorbia perinensis, exhibiting a potent inhibition of peroxidase activity, may be developed as sources of potent antithyroid agents.

• Journal of Environmental Impact Assessment
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• v.20 no.6
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• pp.857-866
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• 2011

This research categorized EIA target highways into following three types in order to minimize non-point source pollution from highway runoff. 1. Big drainage basin. 2. Small drainage basin. 3. Bridge section. The Natural, Filter and Swirl-Type devices were evaluated in terms of removal efficiency of TSS, BOD, COD, T-N, T-P, compatibility of site selection, economic feasibility, and maintenance convenience through which the final BMP was selected. According to the removal efficiency result, the area of Big and Small Drainage basin and bridge section had higher removal efficiency with natural facility than that of the Filter or Swirl-Type device. To make appropriate selection of highways'BMP for non-point source pollution, this study will aim to contribute to building more environmentally friendly highways by proposing the selection process that is made of 5 stages. 1. Selecting the target drainage basin. 2. Selecting the land for the mitigation facility. 3. Analysing the ease of maintenance. 4. Technically evaluating each installation. 5. Evaluating the effective implementation methods.

• Journal of Energy Engineering
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• v.23 no.3
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• pp.7-12
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• 2014

This paper suggest mandatory target predestinator of natural gas wholesale and retail provider will set appropriate target. To analysis natural gas energy saving trend forecast, reduce natural gas forecast and using technology and forecast analysis for equipment is draw based on result of developing tool that more detailed gas field. Also this paper calculate effect on energy saving through various scenarios, efficiency consideration of gas equipment and subsidy condition.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.135-139
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• 2009

In this paper we present INCUI, a user interface based on natural view of physical user interface of target devices and services in pervasive computing environment. We present a concept of Intuitively Natural and Consistent User Interface (INCUI) consisted of an image of physical user interface and a description XML file. Then we elaborate how INCUI template can be used to consistently map user interface components structurally and visually. We describe the process of INCUI mapping and a novel mapping method selection architecture based on domain size, types of source and target INCUI. Especially we developed and applied an extended LCS-based algorithm using prefix/postfix/synonym for similarity calculation.

• 한국지구물리탐사학회:학술대회논문집
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• 2003.11a
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• pp.281-287
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• 2003

A Natural cavities were found at shallow depth during construction of a huge bridge in Moon-Kyung, Korea. The distribution patterns of cavities in the Moon-Kyung limestone were investigated carefully with a supplementary field job such as a structural geological survey, a geophysical survey, and a rock mechanical test in laboratory or field. A structural geological mapping produced a detail geological map on this area. It suggested that there were three faults in this area, and these faults had an influence on the mechanism of natural cavities. Among many kinds of geophysical surveys, an electrical resistivity prospecting was applied firstly on the specific area that was selected by results from the geological survey. Many evidences for cavities were disclosed from this geophysical data. Therefore, a seismic tomography was tested on the target area, which was focused by results from the electrical resistivity prospecting and was believed to have several large cavities. A distinct element numerical simulation using the UDEC was followed on the target area after completing all of field surveys. Data from field tests were directly dumped or extrapolated to numerical simulations as input data. It was verified from numerical analysis that several natural cavities underneath the foundation of the bridge should be reinforced. Based on the project result, finally, most of foundations for the bridge were re-examined and the cement grouting reinforcement was constructed on several foundations among them.

• BMB Reports
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• v.36 no.4
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• pp.344-348
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• 2003

Protein kinase CKII is composed of two catalytic ($\alpha$ or $\alpha$') subunits and two regulatory ($\beta$) subunits. The $CKII{\beta}$ subunit is thought to mediate the tetramer formation and interact with other target proteins. However, its physiological function remains obscure. In this study, point mutants of $CKII{\beta}$ that are defective for the L41 binding were isolated by using the reverse two-hybrid system. A sequence analysis of the point mutants revealed that Asp-26, Met-52, and Met-78 of $CKII{\beta}$ are critical for L41 binding; Asn-67 (and/or Lys-139) and Met-52 are important for $CKII{\beta}$ homodimerization. Two point mutants, R75 and R83, of $CKII{\beta}$ interacted with L5, topoisomerase $II{\beta}$, and CKBBP1/SAG, but not with the wild-type $CKII{\beta}$. This indicates that $CKII{\beta}$ homodimerization is not a prerequisite for its binding to target proteins. These $CKII{\beta}$ point mutants may be useful in exploring the biochemical physiological functions of $CKII{\beta}$.