• Title/Summary/Keyword: Natural regeneration

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Proteomic Assessment of the Relevant Factors Affecting Pork Meat Quality Associated with Longissimus dorsi Muscles in Duroc Pigs

  • Cho, Jin Hyoung;Lee, Ra Ham;Jeon, Young-Joo;Park, Seon-Min;Shin, Jae-Cheon;Kim, Seok-Ho;Jeong, Jin Young;Kang, Hyun-sung;Choi, Nag-Jin;Seo, Kang Seok;Cho, Young Sik;Kim, MinSeok S.;Ko, Sungho;Seo, Jae-Min;Lee, Seung-Youp;Shim, Jung-Hyun;Chae, Jung-Il
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.11
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    • pp.1653-1663
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    • 2016
  • Meat quality is a complex trait influenced by many factors, including genetics, nutrition, feeding environment, animal handling, and their interactions. To elucidate relevant factors affecting pork quality associated with oxidative stress and muscle development, we analyzed protein expression in high quality longissimus dorsi muscles (HQLD) and low quality longissimus dorsi muscles (LQLD) from Duroc pigs by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis. Between HQLD (n = 20) and LQLD (n = 20) Duroc pigs, 24 differentially expressed proteins were identified by LC-MS/MS. A total of 10 and 14 proteins were highly expressed in HQLD and LQLD, respectively. The 24 proteins have putative functions in the following seven categories: catalytic activity (31%), ATPase activity (19%), oxidoreductase activity (13%), cytoskeletal protein binding (13%), actin binding (12%), calcium ion binding (6%), and structural constituent of muscle (6%). Silver-stained image analysis revealed significant differential expression of lactate dehydrogenase A (LDHA) between HQLD and LQLD Duroc pigs. LDHA was subjected to in vitro study of myogenesis under oxidative stress conditions and LDH activity assay to verification its role in oxidative stress. No significant difference of mRNA expression level of LDHA was found between normal and oxidative stress condition. However, LDH activity was significantly higher under oxidative stress condition than at normal condition using in vitro model of myogenesis. The highly expressed LDHA was positively correlated with LQLD. Moreover, LDHA activity increased by oxidative stress was reduced by antioxidant resveratrol. This paper emphasizes the importance of differential expression patterns of proteins and their interaction for the development of meat quality traits. Our proteome data provides valuable information on important factors which might aid in the regulation of muscle development and the improvement of meat quality in longissimus dorsi muscles of Duroc pigs under oxidative stress conditions.

Ischemic Time Associated with Activation of Rejection-Related Immune Responses (허혈 시간과 거부반응 관련 면역반응)

  • Nam, Hyun-Suk;Choi, Jin-Yeung;Kim, Yoon-Tai;Kang, Kyung-Sun;Kwon, Hyuk-Moo;Hong, Chong-Hae;Kim, Doo;Han, Tae-Wook;Moon, Tae-Young;Kim, Jee-Hee;Cho, Byung-Ryul;Woo, Heung-Myong
    • Journal of Veterinary Clinics
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    • v.26 no.2
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    • pp.138-143
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    • 2009
  • Ischemia/reperfusion injury(I/RI) is the major cause of acute renal failure and delayed graft function(DGF) unavoidable in renal transplantation. Enormous studies on ischemia damage playing a role in activating graft rejection factors, such as T cells or macrophages, are being reported. Present study was performed to determine whether ischemia time would play an important role in activating rejection-related factors or not in rat models of I/RI. Male Sprague-Dawley rats were submitted to 30, 45, and 60 minutes of warm renal ischemia with nephrectomy or control animals underwent sham operation(unilateral nephrectomy). Renal function and survival rates were evaluated on day 0, 1, 2, 3, 5 and 7. Immunofluorescence staining of dendritic cells(DCs), natural killer(NK) cells, macrophages, B cells, CD4+ and CD8+ T cells were measured on day 1 and 7 after renal I/RI. Survival rates dropped below 50% after day 3 in 45 minutes ischemia. Histologic analysis of ischemic kidneys revealed a significant loss of tubular architecture and infiltration of inflammatory cells. DCs, NK cells, macrophages, CD4+ and CD8+ T cells were infiltrated from a day after I/RI depending on ischemia time. Antigen presenting cells(DCs, NK cells or macrophages) and even T cells were infiltrated 24 hours post-I/RI, which is at the time of acute tubular necrosis. During the regeneration phase, not only these cells increased but B cells also appeared in more than 45 minutes ischemia. The numbers of the innate and the adaptive immune cells increased depending on ischemia as well as reperfusion time. These changes of infiltrating cells resulting from each I/RI model show that ischemic time plays a role in activating rejection related immune factors and have consequences on progression of renal disease in transplanted and native kidneys.

Comparison of Above-ground Growth Characteristics Between Naturally Regenerated and Planted Stands of Pinus densiflora for. erecta Uyeki in Gangwon Province (천연갱신과 인공조림된 강원지역 금강소나무의 지상부 생장특성 비교)

  • Na, Sung-Joon;Woo, Kwan-Soo;Kim, Chang-Soo;Yoon, Jun-Hyuck;Lee, Heon-Ho;Lee, Do-Hyung
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.323-330
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    • 2010
  • This study was undertaken to compare early growth characteristics, such as height, diameter at root collar (DRC), and the dry weights of stem, branches, needles and roots between naturally regenerated and planted stands of Pinus densiflora for. erecta in Dae-gi and Bo-gwang site, Gangwon province, Republic of Korea. Average DRC growth was similar between the naturally regenerated and the planted stands in Dae-gi area, however, difference was statistically significant (P<0.01) between the naturally regenerated and the planted stands in Bo-gwang area, 4.66 cm and 2.61 cm, respectively. Average height of the naturally regenerated stands was significantly greater (P<0.01) than that of planted stands in both Dae-gi and Bo-gwang areas. The composition ratio of the dry weights for each part of measured trees was the highest in the stem in the naturally regenerated stands, but in the planted stands, the needle was the highest in both sites. The root/shoot ratio in the Dae-gi and Bo-gwang areas in the planted stands were much greater than those in the naturally regenerated stands, 0.25-0.30 and 0.17-0.18, respectively. The ratio of needle to stem biomass (N/S), branch to stem biomass (B/S), and needle to branch biomass (N/B) was significantly greater in the planted stands than those in the naturally regenerated stands in both sites. The results of this study represent that the regeneration methods, natural and artificial regenerations, can have an influence on the early growth of P densiflora for. erecta. This results not only provide the need for a correct planting method, but also will be useful in studies on the environmental adaptability of trees.

The Analysis of Vegetation-Environment Relationship of the Taxus cuspidata Forests by TWINSPAN and DCCA (TWINSPAN 및 DCCA에 의한 한반도(韓半島) 주목림(林)의 군락(群落)과 환경(環境)의 상관관계(相關關係) 분석(分析))

  • Shin, Hyun Chul;Lee, Kang Young;Song, Ho Kyung
    • Journal of Korean Society of Forest Science
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    • v.87 no.4
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    • pp.535-542
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    • 1998
  • This study was carried out for the purpose of supplying the basic data for artificial forestation, natural regeneration and ecological conservation etc., and obtaining information on alpine vegetation, by establishing vegetation units on the basis of phytosociological classification of community and studying growth pattern on the basis of species composition, hierarchy structure and population dynamics, for Taxes cuspidata naturally growing at the alpine districts in Korea. The importance value of Taxus cuspidata by districts mostly showed above 100 in the upper story but at Mt. Chiri and Mt. Hanra communities its value was comparatively low. In the middle story it showed lower value than that of upper story and not quite showed at Mt. Chiri. Taxus cuspidata communities in the lower story were only in Mt. Hanra, Mt. Odae and Mt. Sobaek, and their importance values were about 10 which were relatively low values. The communities were classified into five groups as Taxus cuspidata-Males baccata var. mandshurica, Taxus cuspidata-Abies nephrolepis, Taxus cuspidata-Abies koreana, Taxus cuspidata-Acer mono and Taxus cuspidata-Euonymus quelpaertensis by TWINSPAN analysis. Taxus cuspidata-Abies koreana community was distributed at the northern aspect of the mountain ridges and at higher elevation than other communities and distributed. Taxus cuspidata-Acer mono community was relatively low than the others and distributed at the hillsides of mountain. And Taxus cuspidata-Euonymus quelpaertensis communities were distributed at the relatively high elevation and northern and eastern aspect of the mountain tap, and Taxus cuspidata-Malus baccata var. mandshurica communities were distributed at the medium elevation, and southern and eastern aspect of the mountain ridge. In the relation between communities and environmental factors, it was correlated with aspect, elevation and topography at the first axis, and elevation, slope at the second axis.

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Effects of the Aquatic Vascular Plants on the Lake Ecosystem in the Upper Stream Wetlands of the Namgang-Dam (남강댐 상류 습지에서 수생관속식물이 호소생태계에 미치는 영향)

  • Oh, Kyung-hwan;Lee, Jeong-Hwan;Kim, Cheol-Soo;Son, Sung-Gon;Lee, Pal-Hong
    • Journal of Wetlands Research
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    • v.1 no.1
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    • pp.29-44
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    • 1999
  • Vegetation structure and distribution of the vascular hydrophytes and hygrophytes, and the growth pattern, standing crop and amounts of nutrient uptake by Salix species were investigated in the upper stream wetlands of the Namgang-Dam, Chinju-city, Gyeongsangnam-do, Korea from April to November in 1997. The flora was composed of 43 hydrophytes and 241 hygrophytes, or total 284 vascular plants. The life forms of the hydrophytes were classified as 27 kinds of emergent plants, 4 floating-leaved plants, 3 free-floating plants, and 9 submersed plants. In the herb layer, the dominant species was Persicaria hyciropiper, and the ranges of the species diversity indices (H'), equitabilities, (J') and community similarity indices (CCs) were 1.59~1.89, 0.87~0.96, and 0.35~0.83, respectively. In the shrub and subtree layers, 17 kinds of Salix species were supposed to the pioneer plants at the early stage of the succession. The number of branches per main stem of Salix species was 5.0. The DBH class-frequency histograms of Salix species were the reverse J type, and the natural regeneration of the Salix community was expected. Basal area of Salix species per square meter was $24.87cm^2$. Volume of Salix species per square meter was $12,008cm^3$ and total phytomass of the Salix species was estimated as 12,894 ton. Biomass distribution of Salix species in the stem, the branch and twig, and the leaf was 64.1%, 28.1%, and 7.8%, respectively. The amounts of nitrogen and phosphorus absorbed by Salix species were 68,022 and 19,823 kg. It was recommended that application and conservation of the wetland and other counterplans are indispensable to reduce the adverse effects of water pollution and to preserve the wetland ecosystem.

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Germination and Growth of Oaks (Quercus serrata, Q. mongolica, Q. variabilis) Seedlings by Gradient of Light Intensity and Soil Moisture (광도와 토양수분 구배(勾配)에 따른 참나무류(Quercus Serrata, Q. mongolica, Q. variabilis)치수(稚樹)의 발아 및 성장)

  • Beon Mu-Sup
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.2 no.4
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    • pp.183-189
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    • 2000
  • This study was carried out to analyze ecophysiological responses for seedling of Quercus serrata, Quercus mongolica and Quercus variabilis that are the typical species of deciduous broadleaved forests in Korea. And executed experiments in the climatic control room to provide necessary information to ecological forest management and methods of natural regeneration through the analysis of seedling responses. The details of experimental analysis were growth processes of 4 months after seeding that vary with the condition of three light intensity[relative light intensity(RLI) 8%, 20%, 52%] and three soil moisture[water suction(WS) Ψ=100 hPa, Ψ=280 hPa, Ψ=330 hPa] gradient, growth factors after harvesting and the nutrition condition of leaves. The results of this study are followings: 1) Early growth was prosperous after germination for the species which have more weight of acorn. 2) The formation of lammas shoot was favourable with Q. variabilis and Q. mongolica. And the rate of the occurrence was the highest in the RLI 20%, and it was remarkably reduced in the RLI 8%. 3) As the height growth of seedling of all 3 species were greater in the RLI 20% and 8% than that of the RLI 52%, they showed strong shade tolerance. 4) The increase of light intensity promoted the diameter at root collar growth, and development of main and lateral roots with all 3 species. 5) It showed that the increase of light intensity in the experimental radiation condition raised special leaf area weight(mg/cm$^2$) and leaf area productivity(mg/cm$^2$). Consequently, these resulted in the increase of leaf thickness and total dry biomass per the unit area of leaf. 6) As the increase of light intensity, the minerals contents of leaves such as N, P and K were lowered, and the increase of soil moisture resulted in the increase of P, K, Ca and Mg.

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The Analysis of Forest Vegetation in Mt. Kumjeong (금정산(金井山) 일대(-帶)의 삼림식생분석(森林植生分析))

  • Yun, Chung Weon;Bae, Kwan Ho;Hong, Sung Cheon
    • Current Research on Agriculture and Life Sciences
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    • v.13
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    • pp.31-44
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    • 1995
  • Mt. Kumjeong is located in south-eastern part of the Korean Peninsular($129^{\circ}$01' to $129^{\circ}$05' E, $35^{\circ}$03' to $35^{\circ}$17' N) and the altitude of the summit is 802 meter. In order to find out the vegetation units which made it possible to classify subtropical forest and south-temperate forest, Mt. Kumjeong (which is located in a border of between subtropical forest and south-temperate forest) was selected as a study area. The primary result of actual vegetation analysis in Mt. Kumjeong based upon the vegetation community analysis methods by the ZM-school, and the relationships between vegetation community and two parameters(altitude, topography) analyzed by using coincidence analysis method were summarized as followings. 1. Based upon analytic methods of the vegetation community by the ZM-schools, the primary analytic result of the analysis of vegetation community in Mt. Kumjeong was divided into 10 communities, 9 groups, 2 subgroups. 2. Eurya japonica group of Pinus densiflora community out of 16 vegetation units was thought as a indicator vegetation group which made it possible to border subtropical forest and south-temperate forest. 3. Natural regeneration of Stewartia koreana group was thought to be difficult because seedling and sapling was rarely showed, 4. In relationship between vegetation units and altitude, Carpinus tschonoskii community, Quercus variabilis community, Quercus serrata community, Wisteria floribunda community,. Eurya japonica group of Pinus densiflora community, Pinus thunbergii community mainly distributed below altitude 500 meter, and Quercus mongolica community, Typical group of Pinus densiflora community, Quercus dentata community, Carpinus coreana community, Quercus acutissima community mainly distributed above altitude 500 meter. 5. In relationship between vegetation units and topography, Carpinus tschonoskii community, Quercus variabilis community, Quercus serrata community, Wisteria floribunda community,. Eurya japonica group of Pinus densiflora community distributed below middle slope, Quercus mongolica community, Typical group of Pinus densifiora community, Quercus dentata community, Carpinus coreana community, Pinus thunbergii community Quercus acutissima communily clistributed above midclle slope.

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Production of doubled haploid population derived from the microspore culture of rapeseed (Brassica napus L.) F1 generation and analysis of fatty acid composition (유채 잡종 1세대의 소포자 배양에 의한 배가반수체 집단 선발 및 지방산 조성 분석)

  • Lee, Ji Eun;Park, Ju Hyun;Kim, Kwang Soo;An, Da Hee;Cha, Young Lok
    • Journal of Plant Biotechnology
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    • v.49 no.1
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    • pp.74-81
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    • 2022
  • Brassica napus, an oil crop that produces rapeseed oil, is an allotetraploid (AACC, 2n = 38) produced by natural hybridization between B. rapa and B. oleracea. In this study, microspore was cultured using the F1 developed from a cross between 'EMS26' line with high oleic acid content and 'J8634-B-30' lines. The flower bud size showing the nuclear development at the late uninucleate and binucleate stage with high embryogenesis rate was 2.6 ~ 3.5 mm. Microspores were cultured using only this size and after then most microspore embryo developed into secondary embryos and then regeneration plants obtained from the developed multilobe. The analysis of the ploidy of the plants revealed that 66.7% and 27.8% of the total lines were tetraploids and octoploids, respectively. The sizes of stomatal cells in tetraploids, octoploids, and diploids were 25.5, 35.6, and 19.9 ㎛, respectively, indicating that ploidy level was positively correlated with cell size. Furthermore, 62 tetraploid doubled haploid (DH) lines were selected. The average oleic acid (C18:1) and linolenic acid (C18:3) concentrations of DH were 72.3% and 6.2%, respectively. Oleic acid and linolenic acid concentrations exceeded the two parental values in 5 and 14 DH lines, respectively, suggesting that these two fatty acids had transgressive segregation. Therefore, the DH population can be utilized for the biosynthesis of unsaturated fatty acids in rapeseed and related genes. It can also be used as a breeding material for varieties with high oleic acid concentrations.

Curcumin-induced Cell Death of Human Lung Cancer Cells (Curcumin에 의해 유도되는 인간 폐암 세포주의 세포사멸)

  • Hwasin Lee;Bobae Park;Sun-Nyoung Yu;Ho-Yeon Jeon;Bu Kyung Kim;Ae-Li Kim;Dong Hyun Sohn;Ye-Rin Kim;Sang-Yull Lee;Dong-Seob Kim;Soon-Cheol Ahn
    • Journal of Life Science
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    • v.33 no.9
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    • pp.713-723
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    • 2023
  • Lung cancer is a type of cancer that has the highest mortality rate. It is mainly classified into small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Chemotherapy is used to treat lung cancer, but long-term treatment causes side effects and drug resistances. Curcumin is a bright yellow polyphenol extracted from the root of turmeric. It has biological activities, such as anti-oxidant, anti-cancer, and anti-inflammatory effects. In this study, we observed differential cell death in human lung cancer cells. Based on the results, curcumin at 10, 30, and 50 μM exhibited a dose-dependent inhibition on the cell survival of several lung cancer cells, with minor differential phenotypes. In addition, apoptosis, autophagy, and reactive oxygen species (ROS) regeneration were observed through flow cytometry. Curcumin dose-dependently increased these phenotypes in A549 (NSCLC) and DMS53 (SCLC), which were restored by corresponding inhibitors. Western blotting was performed to measure the level of expression of apoptosis- and autophagy-related proteins. The results indicate that Bax, PARP, pro-caspase-3, and Bcl-2 were dose-dependently regulated by curcumin, with seemingly higher Bax/Bcl-2 ratios in DMS53. In addition, autophagic proteins, p-AKT, p62, and LC3B, were dose-dependently regulated by curcumin. ROS inhibition by diphenyleneiodonium reduced the induction of apoptosis and autophagy generated by curcumin. Taken together, it is suggested that curcumin induces apoptosis and autophagy via ROS generation, leading to cell death, with minor differences between human lung cancer cells.

A Study on Forestation for Landscaping around the Lakes in the Upper Watersheds of North Han River (북한강상류수계(北漢江上流水系)의 호수단지주변삼림(湖水団地周辺森林)의 풍경적시업(風景的施業)에 관(関)한 연구(硏究))

  • Ho, Ul Yeong
    • Journal of Korean Society of Forest Science
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    • v.54 no.1
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    • pp.1-24
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    • 1981
  • Kangweon-Do is rich in sightseeing resources. There are three sightseeing areas;first, mountain area including Seolak and Ohdae National Parks, and chiak Provincial Park; second eastern coastal area; third lake area including the watersheds of North Han River. In this paper, several methods of forestation were studied for landscaping the North Han River watersheds centering around Chounchon. In Chunchon lake complex, there are four lakes; Uiam, Chunchon, Soyang and Paro from down to upper stream. The total surface area of the above four lakes is $14.4km^2$ the total pondage of them 4,155 million $m^3$, the total generation of electric power of them 410 thousand Kw, and the total forest area bordering on them $1,208km^2$. The bordering forest consists of planned management forest ($745km^2$) and non-planned management forest ($463km^2$). The latter is divided into green belt zone, natural conservation area, and protection forest. The forest in green belt amounts to $177km^2$ and centers around the 10km radios from Chunchon. The forest in natural conservation area amounts to $165km^2$, which is established within 2km sight range from the Soyang-lake sides. Protection forest surrounding the lakes is $121km^2$ There are many scenic places, recreation gardens, cultural goods and ruins in this lake complex, which are the same good tourist resources as lakes and forest. The forest encirelng the lakes has the poor average growing stock of $15m^3/ha$, because 70% of the forest consists of the young plantation of 1 to 2 age class. The ration of the needle-leaved forest, the broad-leaved forest and the mixed forest in 35:37:28. From the standpoint of ownership, the forest consists of national forest (36%), provincial forest (14%), Gun forest (5%) and private forest(45%). The greater part of the forest soil, originated from granite and gneiss, is much liable to weathering. Because the surface soil is mostly sterile, the fertilization for improving the soil quality is strongly urged. Considering the above-mentioned, the forestation methods for improving landscape of the North Han River Watersheds are suggested as follows: 1) The mature-stage forest should be induced by means of fertilizing and tendering, as the forest in this area is the young plantation with poor soil. 2) The bare land should be afforested by planting the rapid growing species, such as rigida pine, alder, and etc. 3) The bare land in the canyon with moderate moist and comparatively rich soil should be planted with Korean-pine, larch, ro fir. 4) Japaness-pine stand should be changed into Korean-pine, fir, spruce or hemlock stand from ravine to top gradually, because the Japanese-pine has poor capacity of water conservation and great liability to pine gall midge. 5) Present hard-wood forest, consisting of miscellaneous trees comparatively less valuable from the point of wood quality and scenerity, should be change into oak, maple, fraxinus-rhynchophylla, birch or juglan stand which is comparatively more valuable. 6) In the mountain foot within the sight-range, stands should be established with such species as cherry, weeping willow, white poplar, machilus, maiden-hair tree, juniper, chestnut or apricot. 7) The regeneration of some broad-leaved forests should be induced to the middle forest type, leading to the harmonious arrangement of the two storied forest and the coppice. 8) For the preservation of scenery, the reproduction of the soft-wood forest should be done under the selection method or the shelter-wood system. 9) Mixed forest should be regenerated under the middle forest system with upper needle-leaved forest and lower broad-leaved forest. In brief, the nature's mysteriousness should be conserved by combining the womanly elegance of the lakes and the manly grandeur of the forest.

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