• Journal of Plant Biotechnology
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• v.29 no.2
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• pp.99-104
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• 2002

Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.2
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• pp.187-191
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• 2004

Actin is a ubiquitous and highly conserved protein found in eukaryotic organisms. In this study, we describe the cDNA cloning and mRNA expression of an actin gene from the mulberry longicorn beetle, Apriona germari. The A. germari actin cDNA is 1524 bp containing a complete 1128 bp open reading frame that encodes a polypeptide of 376 amino acid residues with a predicted molecular weight of about 41.5 kDa. The deduced amino acid sequence of the A.germari actin cDNA showed 99% protein sequence identity to Homalodisca coagulata actin, differing at only two amino acid positions, and 92-98% protein sequence identity to known insect species actins. The predicted three-dimensional structure of A. germari actin revealed the four residue hydrophobic pulg loop characteristic of the actin family. Northern blot analysis showed that A. germari actin is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body of A. germari larva.

• Sijohaknonchong
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• v.26
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• pp.55-75
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• 2007

Sijo is the essence of Korean literature and the most ideal poetic form through which we can express our images gracefully in three lines. Hence it deserves special emphasis either in creative writing and appreciating it from elementary school to middle school. In this paper observes how Sijo is taught in the schools and suggests the direction of educating Sijo. There may be three kinds of Sijo performance, namely, recitation, reading, and singing. In this paper. it is claimed that the performance of Sijo in the twenty first century should be recitation. Sijo education may be effective when it focuses on a way of recitation in which, with natural and long breath, a piece of Sijo is recited at length. Nevertheless, it is not practiced as the way of recitation because of following two reasons. Firstly. the analysis on rhythm, which is on the base of its recitation, is extremely difficult. Secondly, the theoretical ways, which is obsolete and lacks vividness, are ineffective in education. By these reasons. 1 studied how to give a recitation following my preceding studies on rhythm and rhythmical reading of Sijo. As a result, this paper suggests a reading method as a solution to the problems. In fact, we Korean can discipline our mind and body through reciting Sijo to the rhythm which is transcendental to Korean and at the same time, Sijo education helps to enhance our pride as koreans in the process of studying Sijo.

• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.225-230
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• 2020

This paper highlights the influence and the importance of the syntactic-grammatical knowledge on "the reading machine", appeared in Jackendoff (1999). Due to the lack of the detailed testing and implementation in his research, this paper tests an extensive data array using a component of Google Translate, currently available freely and most widely on the internet. Although outdated, Jackendoff's paper, "Why can't Computers use English?", argues that syntactic-grammatical knowledge plays a key role in the outputs of computers and computer-based reading machines. The current research has implemented some testings of his thought-provoking examples, in order to find out whether Google Translate can handle the same problems after two decades or so. As a result, it is argued that in the field of NLP, I-language in the sense of Chomsky (1986, 1995 etc) is real and the syntactic, grammatical, and categorial knowledge is essential in the faculty of language. Therefore, it is reassured in this paper that when it comes to human language, even the most advanced "machine" is still no match for human faculty of language, the syntactic-grammatical knowledge.

• Journal of The Korean Association of Information Education
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• v.26 no.6
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• pp.567-577
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• 2022

Interest in AI and SW education is growing as digital literacy is emphasized in the revised elementary school curriculum for 2022. There are numerous restrictions on how pupils can enhance their digital literacy because there are only 34 class hours available for information education in elementary schools. Therefore, other subjects and information education must be blended in order to ensure class hours for AI and SW instruction. In this study, we investigated the impact of S-PUMA reading instruction on the literary imagination and computational thinking of elementary school pupils. To conduct this study, two classes of sixth graders in an elementary school were chosen and split into an experimental group and a control group. Over the course of five sessions, only the experimental group received reading instruction using the S-PUMA teaching approach. It was discovered that reading instruction with the S-PUMA teaching methodology enhanced literary imagination and computational thinking. Further study is required to identify whether the improvement in creative imagination, a component of literary imagination, is a result of the S-PUMA teaching approach or a natural result of the subject matter of the lesson.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.709-714
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• 2017

Structures like bridges or buildings need to be checked continuously to diagnose their safety. However, it is extremely difficult for the people who access such structures to check all areas directly. To overcome this problem, there is a lot of active research into structural health monitoring (SHM) with wireless sensor nodes (WSNs). In this paper, for more accurate checking of SHM with WSNs, we experimentally compare and evaluate the performance of Xenomai, which provides real-time processing under the traditional Linux kernel. For this purpose, we patch Xenomai into the traditional Linux kernel of a commercial embedded board, Raspberry Pi, and implement a task that periodically reads vibration data of the z-axis from an accelerometer in order to analyze the natural frequency of cantilever beams. Reading the data from the traditional Linux kernel with the same method, we analyze the natural frequency of the cantilever beams using Smart Office Analyzer. Finally, to review the validity of Xenomai for WSNs, we obtain vibration data on the z-axis from the accelerometer via wired network and compared and analyzed them the same way.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.2
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• pp.121-126
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• 2001

A cDNA encoding a cathepsin D homologue was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin D homologue of A. germari revealed that the 1,158 bp cDNA has an open reading frame of 386 amino acid residues. The deduced protein sequence of the A. germari cathepsin D homologue shows high homology with cathepsin D in insects, Aedes aegypti (68.2% amino acid similarity) and Drosophila melanogaster (67.2% amino acid similarity). Two aspartic residues and six cystein residues in the A. germari cathepsin D homologue are present at identical locations in all of the other catepsins D. Unlike cathepsins D in two insect species, A. gemari cathepsin D homologue appears to have two putative glycosylation sites, rather than one. Phylogenetic analysis revealed the A. germari cathepsin D homologue is more closely related to insect cathepsins D than to the other animal cathepsins D. Northern blot analysis suggests that A. germari cathepsin D homologue gene is expressed in most if not all, body tissues.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.2
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• pp.217-223
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• 2004

A cathepsin L-like cysteine protease, v-cath, encoded by the baculovirus has been shown to playa role in host liquefaction. We have identified a v-cath gene in the silkworm virus, Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 969 bp v-cath has an open reading frame of 323 amino acids. A putative cleavage site and catalytic sites were conserved in BmNPV-K1 v-cath. The predicted three-dimensional structure of BmNPV-K1 v-cath revealed that the overall fold of BmNPV-K1 v-cath is similar to that of other proteases of the papain family. The deduced amino acid sequence of BmNPV-K1 v-cath showed 98% and 97% protein sequence identity to BmNPV T3 strain and to Autographa californica nuclear polyhedrosis virus, respectively. The BmNPV-K1 v-cath differed at 4 amino acid positions from BmNPV T3. The v-cath gene in BmNPV-K1 genome is located on the EcoRV 6 kb and XhoI 9 kb fragments. Northern hybridization analysis of BmNPV K1 v-cath gene revealed that it is expressed late in infection.

• Journal of Microbiology
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• v.35 no.4
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• pp.277-283
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• 1997

Promoters inducible by paraquat, a superocide-generating agent, were isolated from Escherichia coli using a promoter-probing plasmid pRS415 with promoterless lacA gene. Twenty one promoters induced by paraquat were selected and further characterized. From sequence analysis, thirteen of the promoters were mapped to their specific loci on the Escherichia coli chromosome. Several promoters were mapped to the upstream of known genes such as usgl, katG, and mglB, whose relationships with superoxide response have not been previously reported. Other promoters were mapped to the upstream region of unknown open reading frames. Downstream of HC 96 promoter are uncharacterized ORFs whose sequences are homologous to ABC-transporter subunits. Downstream of HC84 promoter is an ORF encoding a transcriptional regulator-like protein, which contains a LysR family-specific HTH (helix-turn-helix) DNA bindign motif. We investigated whether these promoters belong to the soxRS regulon. All promoters except HC96 were found to belong to the soxRS regulon. The HC96 promoter was significantly induced by paraquat in the soxRS deletion mutant strain. The basal transcription level of three promoters (HE43, HC71, HD94) significantly increased at the stationary phase, implying that they are regulated by RpoS. However, paraquat inducibility of all promoters disappeared in the stationary phase, suggesting that SoxRS regulatory system is active only in rapidly growing cells.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.693-699
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• 2001

An intracellular levansucrase gene, lscR from Rahnella aquatilis ATCC 15552, was cloned and its nucleotide sequence was determined. Nucleotide sequence analysis of this gene revealed a 1,238 bp open reading frame coding for a protein of 415 amino acids. The levansucrase was expressed by using a T7 promoter in Escherichia coli BL21 (DE3) and the enzyme activity was detected in the cytoplasmic fraction. The optimum pH and temperature of this enzyme for levan formation was pH 6 and $30^{\circ}C$, respectively. The deduced amino acid sequence of the lscR gene showed a high sequence similarity (59-89%) with Gram-negative levansucrses, while the level of similarity with Gram-positive enzymes was less than 42%. Multiple alignments of levansucrase sequences reported from Gram-negative and Gram-positive bacteria revealed seven conserved regions. A comparison of the catalytic properties and deduced amino acid sequence of lscR with those of other bacterial levansucrases strongly suggest that Gram-negative and Gram-positive levansucrases have an overall different structure, but they have a similar structure at the active site.