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http://dx.doi.org/10.5010/JPB.2002.29.2.099

Isolation of cDNA Encoding Low Temperature-inducible L-asparaginase from Soybean (Glycin max)  

Park, Seong-Whan (Faculty of Natural Resources and Life science, Dong-A University)
Kim, Kee-Young (Faculty of Natural Resources and Life science, Dong-A University)
Chen, Liang (Faculty of Natural Resources and Life science, Dong-A University)
Lee, Jai-Heon (Faculty of Natural Resources and Life science, Dong-A University)
Publication Information
Journal of Plant Biotechnology / v.29, no.2, 2002 , pp. 99-104 More about this Journal
Abstract
Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.
Keywords
Asparagine; cold acclimation; nitrogen metabolism; suppression subtractive hybridization;
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1 Sutten F, Ding X, Kenefick DG (1992) LEA gene HVA1 regulation by cold acclimation and deacclimation in two barley cultivars with varying freeze resistance. Plant Physiol 99:338-340   DOI   ScienceOn
2 Browse J, Xin Z (2001) Temperature sensing and cold acclimation. Curr Opin Plant Biol 4:241-246   DOI   ScienceOn
3 Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptation to environmental stress. Plant Cell 7:1099-1111   DOI   ScienceOn
4 Borek D, Podkowinski J, Kisiel A, Jaskolski M (1999) Isolation and Characterization of cDNA Encoding L-asparaginase from Lupinus luteus (Accession No. AF112444). (PGR99-050) Plant Physiol 119:1568
5 Casado A, CabaIIero JL, Franco AR, Cardenas J, Grant MR, Munoz-Blanco J (1995) Molecular cloning of the gene encoding the L-asparaginase gene of Arabidopsis thaliana. Plant Physiol 108:1321-1322   DOI   ScienceOn
6 Church GM, Gilbert (1984) Genomic sequencing. Proc Nat'l Acad Sci USA 81:1991-1995   DOI   ScienceOn
7 Ferullo JM, Vezina LP, Rail JR, Laberge S, Nadeau P, Castonguay Y (1997) Differential accumulation of two glycine-rich proteins during cold-acclimation of alfalfa. Plant Mol Biol 33:625-633   DOI   ScienceOn
8 Guy CL (1990) Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol 41:187-223   DOI
9 Hajela RK, Horvath DP, Gilmoure SJ, Thomashow MF (1990) Molecular cloning and expression of cor (cold-regulated) genes in Arabidopsis thaliana. Plant Physiol 93:1246-1252   DOI   ScienceOn
10 Hodges DM, Andrews CJ, Johnson DA, Hamilton RI (1997) Antioxidant enzyme and compound responses to chilling stress and their combining abilities in differentially sensitive maize hybrids. Crop Sci 37:857-863   DOI   ScienceOn
11 Kim JC, Lee SH, Cheong YH, Yoo C-M, Lee SI, Chun HJ, Yun D-J, Hong JC, Lim CO, Cho MJ (2001) A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant J 25:247-259   DOI   ScienceOn
12 Pinhero RG, Rao MV, Paliyath G, Murr DP, Fletcher RA (1997) Changes in activities of antioxidant enzymes and their relationship to genetic and padobutrazol-induced chilling tolerance of maize seedlings. Plant Physiol 114:695-704   DOI
13 Kishitani S, Watanabe K, Yasuda S, Arakawa K, Takabe T (1994) Accumulation of glycinebetaine during cold accumulation and freezing tolerance in leaves of winter and spring barley plants. Plant Cell Environ 17:89-95   DOI   ScienceOn
14 Monroy AF, Castonguay Y, Laberge S, Sarhan F, Vezina LP, Dhindsa RS (1993) A new cold-induced alfalfa gene is associated with enhanced hardening at subzero temperature. Plant Physiol 102:873-879   DOI   ScienceOn
15 Neven LG, Haskell DW, Hofig A, Li Q-B, Guy CL (1993) Characterization of spinach gene responsive to low temperature and water stress. Plant Mol Biol 21:291-305   DOI   ScienceOn
16 Rorat T, Irzykowski W, Grygorowicz WJ (1997) Identification and expression of novel cold induced genes in potato (Solanum tuberosum). Plant Sci 124:69-78   DOI   ScienceOn
17 Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3:217-223   DOI   ScienceOn
18 Shure M, Wessler S, Fedoroff N (1983) Molecular identification and isolation of Waxy locus in maize. Cell 35:225-233   DOI   ScienceOn
19 Sieciechowicz KA, Joy KW, Ireland RJ (1988) The metabolism of asparagine in plants. Phytochemistry 27:663-671   DOI   ScienceOn
20 Sodek L, Lea PJ (1993) Asparaginase from the testa of developing lupin and pea seeds. Phyrochemistry 34:51-56   DOI   ScienceOn
21 Tao D-L, O quist G, Wingsle G (1998) Active oxygen scavengers during cold acclimation of scots pine seedlings in relation to freezing tolerance. Cryobiology 37:38-45   DOI   ScienceOn
22 Solecka D, Boudet A-M, Kacperska A (1999) Phenylpropanoid and anthocyanin changes in low-temperature treated winter oilseed rape leaves. Plant Physiol Biochem 37:491-496   DOI   ScienceOn
23 Someville C (1995) Direct tests of the role of membrane lipidcomposition in low-temperature-induced photoinhibition and chilling sensitivity in plant and cyanobacteria. Proc Nat' l Acad Sci USA 84:739-743
24 Takahashi R, Shimosaka E (1997) cDNA sequence analysis and expression of two cold-regulated genes in soybean. Plant Sci 123:93-104   DOI   ScienceOn
25 Thomashow MF (1998) Role of cold-responsive genes in plant freezing tolerance. Plant Physiol 118:1-7   DOI   ScienceOn
26 Vincze E, Reeves JM, Lamping E, Farnden KJ, Reynolds PH (1994) Repression of the L-asparaginase gene during nodule develop ment in Lupinus angustifolius. Plant Mol Biol 26:303-311   DOI   ScienceOn
27 Verwoerd TC, Dekker BM, Hoekema A (1989) A small-scale procedure for rapid isolation of plant RNAs. Nucl Acid Res 17:2362   DOI   ScienceOn
28 Wanner LA, Junttila (1999) Cold-induced freezing tolerance in Arabidopsis. Plant PhysioI 120:391-400   DOI   ScienceOn
29 Xin Z, Browse UJ (1998) eskimol mutants of Arabidopsis are constitutively freezing-tolelant. Proc Nat'I Acad Sci USA 95:7799-7804   DOI   ScienceOn
30 Moon BY, Hong YN, Kwon YM (1989) Changes in the compositions of amino acids in the rice seedlings under low temperature. Kor J Bot 32:235-245