• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.285-290
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• 2003

Living cells are continuously subject to all sorts of stress such as ultraviolet rays on skin cells. Tests made in various laboratories show that when young fibroblasts (Le. at the beginning of their proliferate life) were repeatedly put under stress at subletal doses, they acquired a phenotype similar to Senescence Induced Prematurely by Stress (SIPS). The work presented hereafter was made on a new model of senescence induced prematurely by stress from ultraviolet Brays (UVB). The human fibroblast model was put under repeated UVB stress, causing SIPS. Several ageing biomarkers were used in order to characterise the cells that underwent stress:. an increase in the proportion of positive cells with senescence associated $\beta$-galactosidase activity (SA $\beta$-gal) measured by a specific coloration,. the proportion in the different morphological stages that fibroblasts undergo during culture visualised by microscopic observation,. the expression of genes known for overexpressing during senescence, particularly fibronectin and apolipoprotein J, measured by Real Time-PCR,. the common deletion of 4,977 bp in mitochondrial DNA, evaluated by nested PCR. Studying the variation of these 4 biomarkers, we have evaluated the protective effect of a Laminaria digitata extract (LDE) that can be used as a natural active ingredient for anti-ageing cosmetics.

• Journal of the Korean Wood Science and Technology
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• v.46 no.4
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• pp.346-354
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• 2018

Kemenyan resin from Styrax sumatrana is a unique non-timber forest product (NTFP) native from Sumatera Island, Indonesia. It possesses a wide range of applications in the pharmaceutical, perfume, and cosmetics industries. In this paper, six kemenyan resin samples were investigated to evaluate their free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) reagent. The kemenyan resin samples, which originated from North Tapanuli, Pakpak Bharat, and Humbang Hasundutan, showed high antioxidant activity with $IC_{50}$ < 16 mg/L. The antioxidant activity of common kemenyan resin constituents, i.e., cinnamic acid, ethyl cinnamate, gallic acid, and vanillin was also investigated as positive control, although they exhibited lower antioxidant activity ($IC_{50}$ < 1000 mg/L), except for gallic acid ($IC_{50}$ = 5,23 mg/L). The total phenolic and flavonoid contents (TPC and TFC) for all samples were 44-66 mg gallic acid equivalents (GAE)/g sample and 143-160 mg quarcetin equivalents (QE)/g sample. The results revealed that kemenyan resin has high potency as an antioxidant and could be used as a natural antioxidant resource.

• The Korea Journal of Herbology
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• v.27 no.2
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• pp.43-46
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• 2012

Objectives : Sanguisorbae Radix(SO) is a plant in the family Rosaceae, which grows widely in open fields Korea. It has been used as traditional medicine for thousands of years, as a treatment for anti-inflammatory and it is widely used for throat infection, tonsilitis, conjuctivitis and lymphadentis. In this study, investigated skin antiaging and anti-bacterial by using SO fractions water, acetone and butanol, chloroform. Methods : The effects of anti-microbial on SO fractions and elastase inhibition activity, collagenase inhibition activity were experimented. Results : 1. The ethyl acetate fraction showed the strongest antimicrobial activity against Staphylococcus epidermidis. 2. The elastase inhibition rate and collagenase inhibition rate of the water fraction of SO was the highest other factions. Conclusions : From the above results, it was confirmed the SO has sufficient potentiality applying itself to industry and also SO can be utilized as antimicrobial natural materials and antiaging cosmetics.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.4
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• pp.263-274
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• 2000

Carotenoids are widely distributed natural pigments which are in an increasing demand by the market, due to their applicatins in the human food, animal feed, cosmetics, and pharmaceutical industries. Although more than 600 carotenoids have been identified in nature, only a few are industrially important (${\beta}$-carotene, astaxanthin, lutein or lycopene). To date chemical processes manufacture most of the carotenoid production, but the interest for carotenoids of biological origin is growing since theire is an increased public concern over the safety of artificial food colorants. Although much interest and effort has been devoted to the use of biological sources for industrially important carotenoids, only the production of biological ${\beta}$-carotene and astaxanthin has been reported. Among fungi, several Mucorales strains, particularly Blakeslea trispora, have been used to develop fermentation processes for the production of ${\beta}$-carotene on almost competitive cost-price levels. Similarly, the basidiomycetous yeast Xanthophyllomyces dendrorhous (the perfect state of Phaffia rhodozyma), has been proposed as a promising source of astaxanthin. This paper focuses on recent findings on the fungal pathways for carotenoid production, especially the structure and function of the genes involved in the biosynthesis of carotenoids in the Mucorales. An outlook of the possibilities of an increased industrial production of carotenoids, based on metabolic engineering of fungi for carotenoid content and composition, is also discussed.

• Bulletin of the Korean Chemical Society
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• v.28 no.8
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• pp.1396-1400
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• 2007

In this study, poly(iso-butyl methacrylate-co-ethylene glycol dimethacrylate) [poly(iso-BMA-co-EGDMA)] microgel was prepared and used as a face powder additive. The spreading, adhesiveness, and skin reactivity of poly(iso-BMA-co-EGDMA) microgel-containing face powder II were investigated and compared with the same properties of commercially available Silica bead 700-containing face powder I. In the results, the particle size of the poly(iso-BMA-co-EGDMA) microgel was significantly swelled as a result of sebum absorption. Face powder II showed a lower primary irritation index and a higher adhesiveness than did face powder I. Face powder I showed a low sebum absorption ratio and a relatively high rate of sebum absorption, whereas face powder II, contrastingly, exhibited a high sebum absorption ratio and a low rate of sebum absorption, which properties would reduce the phenomena of facial strain and sliminess. These results indicate that poly(iso- BMA-co-EGDMA) microgel has outstanding sebum absorption characteristic and adhesiveness, and thus that it is a good candidate for use as a face powder additive.

• Korean Journal of Medicinal Crop Science
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• v.18 no.6
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• pp.373-378
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• 2010

In order to obtain basic data for utilization of the aerial parts of Cnidium officinale Makino (APCO), the antioxidant properties of the aerial parts and rhizomes of C. officinale were measured using DPPH and ABTS radicals, and nitrite scavenging assays. The ethyl acetate (EA) fraction prepared from the aerial parts of APCO showed the strongest antioxidant activities, and contained high level of total phenolic compounds (325.81 mgTE/g) and flavonoids (259.16 mgRE/g). The concentrations for 50% reductions ($RC_{50}$) values of the DPPH and ABTS radicals, and nitrite by the EA fraction of APCO were $11.27\;{\mu}g/m{\ell}$, $14.34\;{\mu}g/m{\ell}$, and $10.26\;{\mu}g/m{\ell}$, respectively. APCO exhibited approximately 3-9 times higher antioxidant activity than rhizomes of C. officinale. The antioxidant capacities of APCO were positively correlated with its total phenolic contents. Therefore, it was concluded that the aerial parts of C. officinale can be a useful and cost-effective source of natural antioxidant for food or cosmetics.

• Archives of Pharmacal Research
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• v.19 no.2
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• pp.163-167
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• 1996

Aloe plants have been used as herbal medicine for centuries and the name aloe means the dried exudate from the cut leaves of Aloe ferox Mill. (Cape aloe, Liliaceae), Aloe ferryi Baker. (Socotrine aloe), A. bainesii Th. Dyer. (Natal aloe) and Aloe vera L. (Curacao aloe) (Namba, 1986). Among them, Aloe vera has not only been one of the most used natural drug well known for its cathartic properties, but also has been widely used as raw materials of cosmetics and health foods (Leung, 1978, Hoffenberg, 1979). Although previous investigations showed that the leaves of A. vera contain a number of anthracene and chromone derivatives such as aloin A, aloin B, 1, 3, 6, 8-tetra-nitro-4,5-dihydroxy-2-hydroxymethyl anthraquinone and 7-hydroxy-aloin, its chemical composition is far from being completely investigated (Hoffenberg, 1979, Rauwald and Voetig, 1982). As a part of our chemical investigations on the constituents of aloe, we report chemical investigation of the freeze dried ground leaves of A. vera which is led to the isolation of five compounds from the ethyl acetate soluble fraction of the methanolic extract.

• Preventive Nutrition and Food Science
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• v.10 no.4
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• pp.326-332
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• 2005

A methanol extract of mulberry cake prepared from mulberry fruits (Morus spp.) was shown to have strong scavenging activities against DPPH, superoxide and hydroxyl radicals. Eleven phenolic compounds were isolated from the mulberry cake by a combination of Diaion HP-20, silica gel (or polyamide), Sephadex LH-20 column chromatographies, preparative HPLC and TLC. Their chemical structures were characterized as procatechuic acid (PCA), caffeic acid (CA), cyanidin 3-O-$\beta$-D-glucopyranoside (CyG) and cyanidin $3-O-\beta­D-rutinoside$ (CyR), rutin (RT), isoquercitrin (IQT), astragalin (AG), quercetin (QT), morin (MR), di-hydroquercetin (DHQ), and 4-prenylmoracin (PM) by spectral analysis and the published data. Most of the phenolic constituents were effective scavengers of DPPH, superoxide and hydroxyl radicals, and especially caffeic acid and 4-prenylmoracin showed potent superoxide and hydroxyl radical scavenging activity, in which their activities were higher than that of the well-known antioxidant, BHT (p< 0.05). Dehydroquercetin and quercetin also exhibited strong superoxide and hydroxyl radical scavenging activities. These results suggest that mulberry cake containing antioxidant phenolic compounds may be useful as natural antioxidants in functional foods and cosmetics.

• Korean Journal of Korean Medical Institute of Dermatology and Aesthetics
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• v.1 no.1
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• pp.91-97
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• 2005

(1) Objectives: To discover natural skin-lightening agents, we have evaluated the inhibitory activity of EtOH extracts from 20 medicinal plants against mushroom tyrosinase. (2) Methods: Tyrosinase activity was determined by the dopachrome method using L-tyrosine as the substrates. (3) Results: Of the plant extracts tested, the extracts of 4 plants, Albizzia julibrissin, Curcuma longa, Anethum graveolens and Sophora flavescens, exhibited potent inhibitory activity (> 50%) in mushroom tyrosinase assay. Four plant extract, extracts of Agrimonia pilosa, Paeonia moutan, Magnolia obovata and Eugenia caryophyllata also showed relatively strong inhibitory (> 40%) against mushroom tyrosinase. (4) Conclusion: These active medicinal plants may be useful for the development of skin-whitening agents. Since the active medicinal plants may contain effective tyrosinase inhibitors even more than kojic acid, further study to identify the active constituents from the plants is expected.

• Natural Product Sciences
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• v.16 no.2
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• pp.107-110
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• 2010

Curcuma caesia Roxb. (Zingiberaceae) is commonly known as 'Black turmeric'. In India it grows in West Bengal, Madhya Pradesh, Orissa, Bihar, North-East and Uttar Pradesh and is widely used by ethnic communities for various ailments. Rhizomes of the plant are used for sprains and bruises and are also employed in cosmetics. In West Bengal it is an important place in traditional system of medicine and is also used as a substitute for turmeric in fresh stage. Present communication deals with the detailed pharmacognostical evaluation of the rhizome sample. Inner part of the rhizome is bluish-black in colour and emits a characteristic sweet smell, due to the presence of essential oil. On steam distillation the rhizome yields an essential oil rich in camphor. A detailed HPTLC studies has been carried out for quantitative evaluation of active marker component. HPTLC, physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of C. caesia rhizome and may possibly help to differentiate the drug from its other allied species.