• Title/Summary/Keyword: Native species

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Endophytes from Natural Festuca spp. in Southwest China and Their Compatibility with Tall Fescue Cultivars (남서중국의 자생페스큐의 엔도파이트와 톨체스큐 품종과의 공생)

  • Wang, Yaoyao;Du, Yongji;Han, Liebao;Li, Deying
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.163-176
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    • 2009
  • Investigating endophyte distribution, naturally occurring in native grasses, is important for understanding endophyte-grass associations and using the beneficial effects of endophytes in cultivated plants. The goal of this study was to investigate endophytes from natural Festuca spp. in Yunnan, Guizhou, and Sichuan Provinces of China, and to study the compatibility between the endophytes and turf type tall fescue (Festuca arundinacea) which is widely used for lawn and athletic fields in that region. Of 628 accessions in F. ovina, 421 had endophytes identified in leaf sheath from on-site microscopic examination. From Festuca spp, three isolates were obtained from the seeds and ninety isolates were obtained from seedlings established from the collected seeds. The isolates from F. ovina and F. stapfii were tentatively identified as Neotyphodium typhinum and Neotyphodiumstarii, respectively. We tested compatibility of the two fungal species with seven tall fescue cultivars, Little Hero, Sub Boy, Eldorado, Arid III, Millennium, Crossfire, and Fawn. N. typhinum or N. starii did not infect 'Fawn' with either seed injection or seed soaking method. The highest infection rate byboth N. typhinum and N. starii was in 'Sun Boy' and 'Eldorado'. There were significant interaction effects between tall fescue cultivar and type of endophyte on infection.

Lytic Action of Egg White Lysozyme Isolated from Ogol Fowl on Staphylococcus aureus Phage Type 29 (Staphylococcus aureus Phage Type 29에 대한 오골계 난백 Lysozyme의 용균성)

  • Oh, Hong Rock;Lee, Jong Soo;Kim, Chan Jo
    • Korean Journal of Agricultural Science
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    • v.14 no.2
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    • pp.286-294
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    • 1987
  • This experiment was carried out to investigate the bacteriolytic action of the egg white lysozyme isolated from Korean native Ogol fowl and to obtain the data for utilization of the enzyme as a food preservative. Staphyococcus aureus phage type 29 and Bacillus subtilis ATCC 6633 among the microorganisms tested were lyzed by the treatment with 0.05% lysozyme, but Staphylococcus aureus phage type 57 in addition to E. coli etc. was found to be a lysozyme- insensitive species. The lysis of S. aureus phage type 29 was maximized when incubated in nutrient broth (pH 7.0) at $37^{\circ}C$ for 24 hours and suspended it to absorbance 0.6 at 540nm in 0.05M sodium acetate but fer (pH 4.5) and then treated it with the 0.05% lysozyme for 30 min. at $30^{\circ}C$. It was found that the effect of 0.05% lysozyme in combination with 1% glycine on the growth inhibition of S. aurecus phage type 29 increased more 50% than that in the absence of glycine, but not effect with other any additeves and metal ions tested.

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Dormancy and Germination Characteristics of Round-Leaved Sundew (Drosera rotundifolia L.) Seeds Native to Korea (자생 끈끈이주걱(Drosera rotundifolia L.) 종자의 휴면과 발아특성)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.564-573
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    • 2016
  • This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.

A Case Study of Biologically Derived Algicidal Substances (Naphthoquinone Derivative) for Mitigate of Stephanodiscus and It's Ecological Changing Monitoring (생물유래 살조물질 Naphthoquinone 유도체의 규조 Stephanodiscus 제어 효과 및 생태계 변화 모니터링: A case study)

  • Joo, Jae-Hyoung;Park, Bum Soo;Kim, Sae Hee;Han, Myung-Soo
    • Ecology and Resilient Infrastructure
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    • v.7 no.1
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    • pp.72-81
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    • 2020
  • Blooms of the small centric diatom Stephanodiscus is quite occasional in winter season in temperate freshwater ecosystems. Often, it leads to degradation of water quality and affects quality of supplied drinking water. In previous studies, naphthoquinone (NQ) compounds have been shown to be effective and selective for controlling winter bloom species Stephanodiscus hantzschii. We conducted a 5 ton scale mesocosm experiment to investigate the effects of NQ on native Stephanodiscus sp. collected from Nakdonggang River in water. After treatment with NQ 4-6 compound (0.2 μM), the cell density of Stephanodiscus sp. was rapidly reduced from 5 × 103 cells mL-1 to 0.2 × 103 cells mL-1 for 10 days. Additionally, NQ 4-6 compound did not affect physicochemical factors (water temperature, dissolved oxygen, pH, conductivity, nutrients) and biological factors (bacteria, heterotrophic nanoflagellates, zooplankton). Therefore, these findings suggest that the NQ 4-6 compound has potential as an alternative algicidal substances to effectively mitigate natural Stephanodiscus sp. blooms, and the application of NQ 4-6 compound will restore the healthy aquatic ecosystems.

Agronomic Characteristics and Herbicidal Response of Barnyard Millet Strains Under Paddy Rice (답리작 적응 조사료용 피의 생육특성 및 제초제 반응)

  • Park, Tae-Seon;Park, Hong-Kyu;Hong, Seong-Woo;Kim, Jeong-Gon;Chung, Nam-Jin;Cho, Hyun-Suk;Seong, Ki-Yeong;Yang, Woon-Ho;Seo, Myung-Chul;Kang, Hang-Won
    • Korean Journal of Weed Science
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    • v.32 no.3
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    • pp.256-262
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    • 2012
  • This experiment was conducted to determine the agronomic characteristics for selection of the excellent barnyard millet and the herbicidal response for their weediness prevention in paddy rice. IT170609, IT195422 and EV2012 have produced more dry matter compared to other barnyard millets tested. In varietal characteristics by heading date, IT170609 showed medium maturing type and IT195422 and EV201 had late maturing type. The germination rate of IT170609, IT195422 and EV2012 on temperature was higher than Echnochloa oryzicola of native species, and it was definitely distinctive at 20 and $25^{\circ}C$. In the herbicidal response for their weediness prevention in paddy rice, imazosulfuron+benzobicyclone+penoxsulam SC showed control effect the extend of over 90% to IT170609, IT195422 and EV2012. Cyhalofop-butyl EC, penoxsulam SC and metamifop EC were very effective by 6~7 leaf stage of barnyard millets tested.

Characteristics of reproductive effect and phenology of Polygonatum stenophyllum grown in riverside in Paju-si (파주시 하천변에 서식하는 층층둥굴레의 계절학과 번식생태학적 특성)

  • Kim, Eui-Joo;Hong, Yong-Sik;Park, Jae-Hoon;Lee, Soo-In;Lee, Eung-Pill;Lee, Seung-Yeon;You, Young-Han
    • Journal of Wetlands Research
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    • v.22 no.2
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    • pp.73-80
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    • 2020
  • Polygonatum stenophyllum Maxim. is a native perennial herb to Korea belonging to family Liliaceae. Although it was removed from the endangered species list, since it inhabits the riverside, it has been threatened with damage to its populations due to frequent disturbances. In order to reveal the basic ecological characteristics of P. stenophyllum, This study was identified the life cycle of the aboveground part and was measured the number of flowers and fruits, which are reproductive organs of P. stenophyllum in riverside in Paju - si during the two years. As a result, aboveground part of P. stenophyllum appeared and grew rapidly until the end of May to about 1m. In early May, two buds per one peduncle were formed up to six pairs per floor. The flowers bloomed in turn from the base of the stem and to the top floor and the blooming occurred when the number of leaves was at least three. Fruits were formed in mid-June and matured by the end of September. The numbers of flowers and fruits were revealed that flowers bloomed in turn from the bottom of the stem to the top floor, the most intensively bloomed in the center part of the stem, and did not bloom from the floor closest to the ground and above the highest 5th node. This basic ecological characteristics of the P. stenophllyum grown in riverside identified in this study will be useful as basic data for their conservation.

Habitat Characteristics and Distribution of Cymbidium kanran Native to Jejudo, Korea (제주한란의 자생환경 특성 및 분포에 관한 연구)

  • Lee, Jong Suk
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.1
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    • pp.40-49
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    • 2004
  • This work carried out to define the characteristic of Cymbidium kanran habitat at Mt. Halla in Jejudo, Korea from the ecological point of view including geological and topographical features, air and soil temperature, relative humidity, fluctuations of light intensity, habitat vegetation, distribution altitude, area limit, and etc. And another goal of this study consider the conservation counterplan of the cymbidium habitat. Natural distribution areas of the cymbidium were observed more abundantly on the well drained south and east-facing slopes. Soil acidity was ranged from pH 4.1 to 5.3, and electric conductivity was ranged from 176.4 to 299.9 us/cm (average 215.3 us/cm). Base-saturation percentage of the habitat soil was below 50%, bulk density 0.42g/$cm^2$, particle density 2.05g/$cm^2$, humus content 26%, total nitrogen 0.82%, available phosphate 4.2 mg/kg, exchangeable potassium 0.63 Cmol/kg, calcium 0.44 Cmol/kg and magnesium 0.67 Cmol/kg. Annual mean air temperature was $15.4^{\circ}C$, however, air temperature was ranged $11.7{\sim}18.2^{\circ}C$ in spring, $21.2{\sim}23.8^{\circ}C$ in summer, $12.8{\sim}22.0^{\circ}C$ in fall and $5.5{\sim}7.8^{\circ}C$ in winter season. Annual mean soil temperature at depth of 10cm was $13.2^{\circ}C$ And minimum value was recorded $4.7^{\circ}C$ on January, and maximum value $22.5^{\circ}C$ on August. Relative humidity was ranged 90.8~94.7% in summer, 80.8~91.5% in fall and 77.6~84.2% in winter season. Minimum value was 56.5% on December, and maximum value was 100% on July and August. Light intensities were ranged from 400 to 1,800 lux at the greater part of Cymbidium kanran sites in Jejudo. Summer regarded as an lower light intensities was recorded to be range of 500~600 lux; however, autumn and winter were shown higher light regimes ranged from 3,500 to 3,800 lux. Therefore, one must be suprised that the cymbidium grow at the light condition of 6 lux (minimum) or 10,000 lux (maximum). Tree species keeping higher frequency rate and density were Eurya japonica, Camellia japonica, Castanopsis cuspidata, Carpinus laxiflora and Pinus densiflora. Number of trees growing in a 5${\times}$5m quardrat was 35 as an average, and proportion of evergreen versus deciduous was 5:1. Distribution altitude of the orchid habitat was ranged from 120m (low) to 840m (high) from sea level on the south facing slope of Mt. Halla, and was ranged eastern borderline of Gujwaup, Bukjejugun to western boundaries of Jungmundong, Seogwipo city. For the stable conservation of Cymbidium kanran habitat, sunlight regimes must be increased more by means of cutting trees or twigs in the site.

Occurrence of Enterocytozoon bieneusi in Korean Native Cattle Examined by Light Microscopic and Molecular Methods (광학 현미경 및 분자생물학적 방법을 적용한 한우의 Enterocytozoon bieneusi 역학조사)

  • Lee, John-Hwa;Kim, Nam-Soo;Jeon, Byung-Woo;Son, Hwa-Young;Ryu, Si-Yun;Shin, Hyun-Jin;Park, Jie-Yeun;Kim, Hyeon-Cheol;Hur, Jin;Cho, Jeong-Gon;Park, Bae-Keun
    • Journal of Veterinary Clinics
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    • v.27 no.1
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    • pp.1-5
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    • 2010
  • Enterocytozoon bieneusi, a microsporidian species, has emerged as an opportunistic pathogen in AIDS patients. This organism has also been identified in a wide range of animals, and the zoonotic potential of human infections is of particular interest. This study revealed that this organism was found with relatively high prevalence in feces of asymptomatic cattle in Korea. Fecal specimens were obtained from a total of 1,720 cattle in a slaughterhouse located in Chungnam province, Daejeon city and Chonbuk province. After removal of fecal debris by sieving and density gradient centrifugation, samples were examined by microscopic examination and then nested polymerase chain reaction (PCR). Microscopic examination with the modified trichrome staining for the fecal specimens revealed 194 (11.28%) positive calves for microsporidia spore. PCR using the specific primer for E. bieneusi revealed 79 (4.59%) positive calves. The infection ratio of microsporidia was higher in March than other season.

Antifungal Activity on the Water Extracts of Five Fagaceae Plants (참나무과 수목 5종 수용성 추출물의 항균활성)

  • Moon, Sang-Ho;Song, Chang-Khil;Kim, Tae-Keun;Oh, Dong-Eun;Kim, Hyoun-Chol
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.295-310
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    • 2017
  • This study investigated the growth of five phytopathogenic fungi including Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici and others according to different concentrations of water extract in order to provide reference data for developing environment-friendly agricultural materials using five native Fagaceae species including Quercus acuta, Quercus salicina, Quercus glauca, Quercus gilva and Castanopsis cuspidata var. sieboldii. As the concentration of aqueous extracts of Fagaceae increased according to donor plants, the mycelial growth of phytopathogens showed a decreasing tendency. Differences were found in the degree of inhibition according to types of donor plants and pathogenic fungi. Diaporthe citri, Phytophthora capsici, Pythium graminicola on the water extract of Castanopsis cuspidata var. sieboldii inhibited mycelial growth by 84% in 25% of the treatment group and by 87% in more than half of the treatment group. The water extract of Quercus acuta was found to have no inhibitory effect against the mycelial growth of Diaporthe citri. The aqueous extracts of Quercus salicina, Quercus glauca and Quercus gilva insignificantly inhibited mycelial growth by approximately 15%. The total phenolic content of receptor plants exhibiting antifungal activity was highest in Castanopsis cuspidata var. sieboldii with a content of 22.32 mg/g phenols, followed by Quercus salicina with 8.32 mg/g, Quercus glauca with 6.83 mg/g, Quercus gilva with 5.95 mg/g, and Quercus acuta with 5.24 mg/g. The aqueous extracts of Castanopsis cuspidata var. sieboldii among the five Five Fagaceae Plants of were the most effective antifungal activity.

Ecotoxicological Test on Various Industrial Effluent Using Mayfly Egg, Ephemera orientalis (동양하루살이 알을 이용한 산업폐수 생태독성평가)

  • Mo, Hyoung-ho;Son, Jino;Jung, Jinho;Shin, Key-Il;Cho, Kijong
    • Korean Journal of Environmental Biology
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    • v.34 no.3
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    • pp.212-215
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    • 2016
  • We developed a new ecotoxicological test method using native test species, eggs of Ephemera orientalis, and five kinds of industrial wastewater were tested to validate the test method. The water samples were collected in Jun 2006 from the following industries: pesticide, metal plating, PCB, leather1, and leather2. Wastewater and effluent were diluted by distilled water, respectively, to prepare various concentrations, 100, 50, 25, 12.5, 6.3, 3.1, and 0%. For the egg bioassay, 20 freshly laid eggs (<24 h old) were exposed to test solutions in a Petri dish ($52{\times}12mm$) at $20^{\circ}C$ with photoperiod of 16 h light and 8 h dark for 14 days. The median egg hatching concentrations (EHC50) were estimated using Probit analysis. All EHC50s of wastewater were less than 3.1%, which meant very high ecotoxicity except for the wastewater of PCB industry having 6.1% of EHC50. Among the effluents, the least toxic effluent was from pesticide industry having 58% of EHC50, while the effluent of leather2 was the most toxic having 7.3% of EHC50.