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http://dx.doi.org/10.11626/KJEB.2016.34.3.212

Ecotoxicological Test on Various Industrial Effluent Using Mayfly Egg, Ephemera orientalis  

Mo, Hyoung-ho (Institute of Life Science and Natural Resources, Korea University)
Son, Jino (Division of Environmental Science and Ecological Engineering, Korea University)
Jung, Jinho (Division of Environmental Science and Ecological Engineering, Korea University)
Shin, Key-Il (Department of Statistics, Hankuk University of Foreign Studies)
Cho, Kijong (Division of Environmental Science and Ecological Engineering, Korea University)
Publication Information
Korean Journal of Environmental Biology / v.34, no.3, 2016 , pp. 212-215 More about this Journal
Abstract
We developed a new ecotoxicological test method using native test species, eggs of Ephemera orientalis, and five kinds of industrial wastewater were tested to validate the test method. The water samples were collected in Jun 2006 from the following industries: pesticide, metal plating, PCB, leather1, and leather2. Wastewater and effluent were diluted by distilled water, respectively, to prepare various concentrations, 100, 50, 25, 12.5, 6.3, 3.1, and 0%. For the egg bioassay, 20 freshly laid eggs (<24 h old) were exposed to test solutions in a Petri dish ($52{\times}12mm$) at $20^{\circ}C$ with photoperiod of 16 h light and 8 h dark for 14 days. The median egg hatching concentrations (EHC50) were estimated using Probit analysis. All EHC50s of wastewater were less than 3.1%, which meant very high ecotoxicity except for the wastewater of PCB industry having 6.1% of EHC50. Among the effluents, the least toxic effluent was from pesticide industry having 58% of EHC50, while the effluent of leather2 was the most toxic having 7.3% of EHC50.
Keywords
ecotoxicity; egg hatching; wastewater;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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1 Beketov M. 2004. Different sensitivity of mayflies (Insecta, Ephemeroptera) to ammonia, nitrite and nitrate: linkage between experimental and observational data. Hydrobiologia 528:209-216.   DOI
2 Hamilton MA, RC Russo and RV Thurston. 1977. Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays. Environ. Sci. Technol. 11:714-719.   DOI
3 Hwang JM, YJ Bae and WP McCafferty. 2007. A checklist of the burrowing mayfly family Ephemeridae. pp. 159-172. In International Advances in the Ecology, Zoogeography and Systematics of Mayflies and Stoneflies (Hauer FR, JA Stanford and RL Newell eds.). University of California Press. Berkeley.
4 Hwang JM, TJ Yoon, SJ Lee and YJ Bae. 2009. Life history and secondary production of Ephemera orientalis (Ephemroptera: Ephemeridae) from the Han River in Seoul, Korea. Aquat. Insects 31:333-341.   DOI
5 Kim BS, YK Park, KH Park, JS Shin and JH Kim. 2005. Optimal temperature conditions of Korean freshwater Cladoceran for development of standard toxicity test methods. Korean J. Pestic. Sci. 9:221-230.
6 Ministry of Environment. 2006. Standard methods for the examination of water and wastewater. Ministry of Environment.
7 Ministry of Environment. 2009. Development and standardization of ecotoxicity tests using species indigenous to Korea (III). Ministry of Environment.
8 Mo HH, Y Kim, YS Lee, YJ Bae, JS Khim and K Cho. 2016. Burrowing mayfly Ephemera orientalis (Ephemeroptera: Ephemeridae) as a new test species for pesticide toxicity. Environ. Sci. Pollut. Res. 23:18766-18776.   DOI
9 National Institute of Environmental Research. 2014. Guideline of ecotoxicological test and operation. National Institute of Environmental Research.
10 De Gelas K and L De Messter. 2005. Phylogeography of Daphnia magna in Europe. Mol. Ecol. 14:753-764.   DOI
11 Zhou Q, J Zhang, J Fu, J Shi and G Jiang. 2008. Biomonitoring: an appealing tool for assessment of metal pollution in the aquatic ecosystem. Anal. Chim. Acta. 606:135-150.   DOI
12 Van Wijingaarden RPA. 1993. Comparison of response of the mayfly Cloeon dipterum to chlorpyrifos in a single species toxicity test, laboratory microcosm, outdoor ponds and experimental ditches. Sci. Total Environ. 134:1037-1046.   DOI
13 Wui IS, CH Ra, JB Lee and SK Baik. 1991. Studies on the bioindicator of aquatic insects to the environmental pollution. Korean J. Environ. Biol. 9:42-54.