• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.87-93
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• 2011

S-Adenosylmethionine decarboxylase (SAMDC; EC 4.1.4.50), a key enzyme for polyamines biosynthesis, was tightly regulated for homeostatic levels. Carnation SAMDC gene (CSDC9) has an small upstream open reading frame (uORF) of 54 amino acids in 5'-leader sequence. To explore the functional mechanism of uORFs in controlling translation, we used a GUS reporter gene driven with the 35S promoter and uORF region of SAMDC gene for making transgenic tobacco plants. In our experiment, there were a translational inhibition of its downstream GUS ORF by SAMDC uORF sequence or SAMDC uORF protein. Expecially, translational inhibition was most effective in point-mutated construct, in which the start codon was changed. Therefore, this results suggested the ribosomal stalling might be involved in this translational inhibitory process. The frame shift in amino acid sequence of SAMDC uORF with start codon and stop codon resulted in a moderate increasing in GUS activity, suggesting the native amino acid sequence was important for a function as a translational inhibitor. Also, we showed that the production of GUS protein was significantly inhibited in the presence of the small uORF using histochemical analysis of GUS expression in seedlings and tobacco flowers. Importantly, the small uORF sequence induced a real peptide of 5.7 kDa, which was provided the presence of SAMDC uORF peptide band using an in vitro transcription/translation system. The peptide product of uORF might interact with other components of translational machinery as well as polyamines, which was resulted from that polyamine treatment was inhibited GUS protein band in SDS-PAGE experiment.

• Journal of Korean Society of Rural Planning
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• v.26 no.1
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• pp.77-88
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• 2020

This study had comparatively analyzed the influence factors of the farm village business on the local farmhouses centered on the level of importance and the level of performance. For this, a questionnaire survey had been conducted with 350 local residents who had participated in the Rural Field Forum of 14 villages, including Gyeongsangnam-do, Gyeongsangbuk-do, Chungcheongnam-do, Chungcheongbuk-do, Jeollabuk-do, etc. Excluding the insincere survey questionnaires, the final 232 people had been analyzed. As a result of the analysis of this study, the factors that have the influences on the diverse, rural areas had been compressed into the three factors of the lodging and the program, the special, rural area products, and the transportation and the accessibility. In the level of importance-level of performance analysis, it had been analyzed that the special, rural area products must maintain the continuity, that the transportation accessibility must avoid the excessive endeavor, and that the lodging program had a low priority order. Therefore, it can be said that it is necessary to concentrate the capability of the rural village business in the direction of developing the local specialties in the future. Next, after dividing the level of performance and the level of importance into the two groups each, the multiple group path analysis had been carried out. As a result, in the result of the analysis of the path that has an influence on the economic factor, the path coefficient of the level of importance of the transportation accessibility -> profit increase was 0.195+0.064 and the path coefficient of the level of performance of the transportation accessibility -> profit increase was 0.251+0.074. Therefore, both of them had the statistically significant amounts of the influences. This suggests that the transportation accessibility is an important element of the profit increase of the region. As a result, in the future, the research on the urban and rural exchanges using the even more convenient and inexpensive transportation means is needed. Most of them need the voluntary effort of the residents for being able to creatively develop and sell the native, agricultural, specialty products that are petty. Also, the rural areas need the diversities of the lodging programs and the recreational programs, a convenient reservation system, and the development of a unique tourism experience program. In the future, the researches that had been advanced in the even more detailed domain that is related to these factors are highly expected. Also, the analyses that had been carried out with even more areas and subjects as the targets are needed.

• Journal of the Korean association of regional geographers
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• v.3 no.2
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• pp.227-253
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• 1997

Twenty-first century will be in the era of culture and art as postmodernims progresses, therefore, geography which is is traditionally concerned with regional study needs to deeply study the culture of region. Regional symbols are clue to identify regional culture which is environments of symbolic space. Especially the study of urban culture come to the needs of times with occurring global city, urban symbols make clear urban culture and urban identity. The aim of this paper is to analyze urban symbols, that is urban songs, urban birds, urban trees, urban flowers to study urban culture in korean cities, urban symbols make clear urban spatial environment which is natural and human, and then, urban symbols make clear urban identity. The analytical results of lyrics of urban songs in the 76 cities of korea include names of mountain, river, sea and place, the names of mountain and river make clear urban identies which is natural and human characteristics, it is environment of symbolic space which brings unity, one body. Especially it is environment of symbolic space which comprise fung-soo(風水) aspects, place names well represent symbolic space. The analytical results of symbolic things, that is urban birds, urban trees, urban flowers in the 78 cities of korea indicate they are gain and gain established, they are also our environments of symbolic space which is curtual settlings of our life, but they are imported not native species but foreign species which have not environment of symbolic space. The results of this paper implicate urban symols should review. because urban symbols fit to our natural and human environments of symbolic space.

• Korean Journal of Environmental Biology
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• v.34 no.3
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• pp.169-176
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• 2016

Although chemical fertilizers have a quick effect and broad applicability to agricultural fields, they have caused many problems like increasing soil acidity or decreasing soil organic matters. Environmental-friendly agriculture has been attempted in various ways such as organic agriculture, natural farming, low input and sustainable agriculture. The common interest of all environmental-friendly systems is to decrease burden to agricultural environment by low input of agricultural labor and materials. This study was conducted to estimate overwintering capacity and genetic distance among Chinese Milk Vetch (Astragalus sinicus, CMV) collections based on morphological characteristics and AFLP (Amplified fragment length polymorphism) analysis. Furthermore, the effect of CMV as green manure was observed in mix-cultured paddy fields with rice, sesame and sweet-potato. An another objective of this study was also to compare the pattern of weed occurrence in paddy fields with or without CMV and different rice transplanting times. The CMV collected from Paju district in central region of Korea was successively occurring through self-reseeding without artificial management. However, there was no noticeable difference in growth habit between Paju native CMV and introduced CMV from China which is currently used in farm fields. On the basis of multi-dimensional scaling and tree analyses, there are no significant difference of agricultural growth characteristics among Paju and chinese collections only excepting leaf angle and root length. The flowering time of Gurye collection was fast for 1 week as compared to other collections. AFLP that was commonly used for plant classfication, was applied to exam the genetic variation of CMV collections. Total 579 PCR products and 336 polymorphic fragments were generated using 8 primer pairs.

• Korean Journal of Veterinary Research
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• v.31 no.1
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• pp.109-121
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• 1991

This study was carried out to investigate the morbidity of fatty liver in cattle at the abattoir and on the farm, and to cytodiagnose fatty liver in cattle by fine needle aspiration biopsy. Incidence rates of fatty liver in cattle, detected macroscopically or based on hepatic lipid content by buoyancy, were 0.30% in Korean native cows, 4.70% in dairy cows, and 0.15% in dairy bull. Fatty liver was enlarged, swollen with round edges, light weight, and pale to yellow-orange color, but its color was not always correlated to the severity of fatty liver. The findings of fat infiltration of the hepatic lobule were large droplets around central vein, fine droplets in the periphery, and fat infiltration in the perivascular region execpt for most of normal liver and severe fatty liver. The sensitivty, specificity, and accuracy of cytological finding compared with hepatic lipid content by buoyancy were 94.4%, 95.2%, and 94.9% in normal cases, 64.3%, 100%, and 87.2% in mild cases, 100%, 83.3%, and 87.2% in moderate cases, and 100%, 100%, and 100% in sesvere cases, respectively. Cytological findings were well correlated with histological findings. Complications of fine needle aspiration biopsy were not recognized clinically. Consequently, the cytodiagnosis by fine needle aspiration biopsy is simple, rapid, safe, and economical method compared with histological techniques in the diagnosis of fatty liver in cattle.

• Journal of architectural history
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• v.3 no.1
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• pp.57-82
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• 1994

This paper is the result of the researches and the field surveys of the villages and the dwellings of Korean immigrants in Yien-Pien area, north-eastern China. This study aims to persue both of the origin and the process of development of their settlements and dwelling types from late 19C to the present. Their processes are too complex to analysis by single view-point. I have eyes to interprete them from three pionts; 1)correspondences between the dwelling types and the econo-political history of their region, 2)cultural assimilation with the native dwelling types, and 3)the direction of their modernization with the economical development of modern China. Three village types have been pioneered; 1)the villages of indivisual immigration, 2)the villages of planned group immigration, and 3)the villages of socilistic reform villages of 1) were composed of organic village patterns and various shaped dwelling lots on the sloped site; villages both of 2) and 3), gird patterns and uniformed lots on open fields. Historically, villages of 1) were pioneered before 1931; villages of 2), 1936-1945; villages of 3), from 1945. Each of dwelling types had strong relations with the village types to which it belonged. Before 1931, dwellings were built up based on so called "Ham-buk dwelling type" which was dominent in north-eastern Korea. In the era of gruop-immigration, various dwelling types were flew into Yen-Pien from southern Korea. In modern China, their southern types were changed into Yen-Pien type as similar as Ham-book type. After 1945, with the Great leap Forward and the Cultural Revolution, as communization of indivisual properties and reorganization of rural communities, each of dwellings became smaller and simpler in aspects of scales as well as functions. There are two types in Yen-Pien dwellings, those are 'single-file' and 'double-file' type. Three sub-types of latter arc 'six-bays', 'eight-bays', and rarely 'ten-bays'. The most common element of all types is Chong-ju-k'an; which is large room with heated floor, openig to kitchen. Now, modern dwellings of Korean immigrants are changing their spatial compositions, materials, and structures. With cultural assimilation as well as modernization, especially in urban areas, they are compelled to accept the elements of Chinese dwellings. But the spatial element of "Chong-ju-k'an", which is the core element of Yen-Pien dwelling type, never fade away nor is changed.

• Mycobiology
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• v.43 no.4
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• pp.373-383
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• 2015

Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

• The Korean Journal of Ecology
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• v.28 no.6
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• pp.375-382
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• 2005

The American bullfrog, Rana catesbeiana was imported from Japan for farming for the human consumption in 1970's and introduced populations were a great threat to native habitats in the pond and lake ecosystem. However, it is thought that the population of bullfrog has rapidly declined for past years in Korea. In this study, we investigated the intra-genetic diversity of R. catesbeiana habitated in Korea. The nucleotide sequences of 1,215bp mitochondrial ND1/tRNA region in bullfrogs sampled from 5 sites in Jeollanamdo were analyzed and compared to the original sequence of R. catesbeiara reported in Genbank. The nucleotide similarity between Korean and North American bullfrog was ranged from 98.7% to 100% based on kimura-2-parameter distance. In addition, bullfrogs analyzed in this study were clustered into two groups with one including Jangheung and the other including Gwangju populations in the neighbor-joining tree. North American R. catesbeiana was grouped in Jangheung cluster, indicating that there is the very low genetic difference between Korean and North American populations. The maximum parsimony tree in which North American R. catesbeiana was set as an outgroup suggests that Jangheung group represents the introduced population to Korea. Taken together, the results indicate that the population of R. catesbeiana in Korea has not segregated geographically yet, after the introduction.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.1
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• pp.72-83
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• 1997

Forty six natural habitats of Korean lespedeza(Leapedeza stipulacea Maxim.) were investigated for the growth characteristics, mineral contents of plant and the physico-chemical properties of natural habitat's soil compared with the upland soil nearby the habitat. The results obtained were summarized as follows. Plant height and dry matter yield were higher in the samples taken in late than early August showing large variation within the sampling date and location. Korean lespedeza showed higher contents of Fe and Mn, but lower contents of K, Ca, Mg and Cu than Alfalfa. The contents of P and Zn were about the same. The natural habitat showed higher soil pH and Ca content but lower contents of $P_2O_5$, K, and organic matter than the vicinity of natural habitats and the average soil of Korea. The contents of Mg were about the same. The soil texture of natural habitats showed much higher portion of sand and extremely low portions of silt and clay than both area. Korean lespedeza is so well adapted to the soil with low fertilizer and organic matter that other crops and most weeds fail. It also appears to thrive on the sandy loams, loamy sands of the piedmont region such as waste land and on the similar soils with variously physico-chemical properties.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.405-413
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• 2016

Quinoa (Chenopodium quinoa Willd.) is a plant native to the Andean region that has become increasing popular as a food source due to its high nutritional content. This study determined the optimal photoperiod, light intensity, and electrical conductivity (EC) of the nutrient solution for growth and yield of quinoa in a closed-type plant factory system. The photoperiod effects were first analyzed in a growth chamber using three different light cycles, 8/16, 14/10, and 16/8 hours (day/night). Further studies, performed in a closed-type plant factory system, evaluated nutrient solutions with EC (salinity) levels of 1.0, 2.0 or $3.0dS{\cdot}m^{-1}$. These experiments were assayed with two light intensities (120 and $143{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) under a 12/12 and 14/10 hours (day/night) photoperiod. The plants grown under the 16/8 hours photoperiod did not flower, suggesting that a long-day photoperiod delays flowering and that quinoa is a short-day plant. Under a 12/12 h photoperiod, the best shoot yield (both fresh and dry weights) was observed at an EC of $2.0dS{\cdot}m^{-1}$ and a photosynthetic photon flux density (PPFD) of $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. With a 14/10 h photoperiod, the shoot yield (both fresh and dry weights), plant height, leaf area, and light use efficiency were higher when grown with an EC of $2.0dS{\cdot}m^{-1}$ and a PPFD of $143{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Overall, the optimal conditions for producing quinoa as a leafy vegetable, in a closed-type plant factory system, were a 16/8 h (day/night) photoperiod with an EC of $2.0dS{\cdot}m^{-1}$ and a PPFD of $143{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$.